High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system.
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| Title: | High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system. |
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| Authors: | Sharp, V. Scott1 vss@lilly.com, Gokey, Megan A.2, Wolfe, Chad N.3, Rener, Gregory A.1, Cooper, Mary R.2 |
| Source: | Journal of Chromatography A. Oct2015, Vol. 1416, p83-93. 11p. |
| Subjects: | High performance liquid chromatography, Chiral stationary phases, Drug use testing, Elution (Chromatography), Carboxylates, Esters |
| Abstract: | Using multiple HPLC chromatographic modes and various chiral columns in the context of an automated screening system, a potential separation was initially identified for the methyl ester of evacetrapib and its stereoisomers using an immobilized polysaccharide-based HPLC column. The bonded nature of this column, the Chiralpak ® IC, allows for enhanced separation development with a diverse solvent range not amenable to standard coated chiral stationary phases. The ternary eluent system ultimately identified provided isomer resolutions not obtainable via the more established hexane/alcohol or polar organic chromatographic modes. A systematic separation development process is described, first for the resolution of the isomers, and later incorporating five potential impurities. A robust separation system was eventually developed that effectively resolves all compounds within a reasonable analysis time. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 109915583 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Sharp%2C+V%2E+Scott%22">Sharp, V. Scott</searchLink><relatesTo>1</relatesTo><i> vss@lilly.com</i><br /><searchLink fieldCode="AR" term="%22Gokey%2C+Megan+A%2E%22">Gokey, Megan A.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wolfe%2C+Chad+N%2E%22">Wolfe, Chad N.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Rener%2C+Gregory+A%2E%22">Rener, Gregory A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cooper%2C+Mary+R%2E%22">Cooper, Mary R.</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Oct2015, Vol. 1416, p83-93. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Chiral+stationary+phases%22">Chiral stationary phases</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+use+testing%22">Drug use testing</searchLink><br /><searchLink fieldCode="DE" term="%22Elution+%28Chromatography%29%22">Elution (Chromatography)</searchLink><br /><searchLink fieldCode="DE" term="%22Carboxylates%22">Carboxylates</searchLink><br /><searchLink fieldCode="DE" term="%22Esters%22">Esters</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Using multiple HPLC chromatographic modes and various chiral columns in the context of an automated screening system, a potential separation was initially identified for the methyl ester of evacetrapib and its stereoisomers using an immobilized polysaccharide-based HPLC column. The bonded nature of this column, the Chiralpak ® IC, allows for enhanced separation development with a diverse solvent range not amenable to standard coated chiral stationary phases. The ternary eluent system ultimately identified provided isomer resolutions not obtainable via the more established hexane/alcohol or polar organic chromatographic modes. A systematic separation development process is described, first for the resolution of the isomers, and later incorporating five potential impurities. A robust separation system was eventually developed that effectively resolves all compounds within a reasonable analysis time. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.chroma.2015.09.013 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 83 Subjects: – SubjectFull: High performance liquid chromatography Type: general – SubjectFull: Chiral stationary phases Type: general – SubjectFull: Drug use testing Type: general – SubjectFull: Elution (Chromatography) Type: general – SubjectFull: Carboxylates Type: general – SubjectFull: Esters Type: general Titles: – TitleFull: High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Sharp, V. Scott – PersonEntity: Name: NameFull: Gokey, Megan A. – PersonEntity: Name: NameFull: Wolfe, Chad N. – PersonEntity: Name: NameFull: Rener, Gregory A. – PersonEntity: Name: NameFull: Cooper, Mary R. IsPartOfRelationships: – BibEntity: Dates: – D: 16 M: 10 Text: Oct2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 1416 Titles: – TitleFull: Journal of Chromatography A Type: main |
| ResultId | 1 |
