High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system.
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| Title: | High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system. |
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| Authors: | Sharp, V. Scott1 vss@lilly.com, Gokey, Megan A.2, Wolfe, Chad N.3, Rener, Gregory A.1, Cooper, Mary R.2 |
| Source: | Journal of Chromatography A. Oct2015, Vol. 1416, p83-93. 11p. |
| Subjects: | High performance liquid chromatography, Chiral stationary phases, Drug use testing, Elution (Chromatography), Carboxylates, Esters |
| Abstract: | Using multiple HPLC chromatographic modes and various chiral columns in the context of an automated screening system, a potential separation was initially identified for the methyl ester of evacetrapib and its stereoisomers using an immobilized polysaccharide-based HPLC column. The bonded nature of this column, the Chiralpak ® IC, allows for enhanced separation development with a diverse solvent range not amenable to standard coated chiral stationary phases. The ternary eluent system ultimately identified provided isomer resolutions not obtainable via the more established hexane/alcohol or polar organic chromatographic modes. A systematic separation development process is described, first for the resolution of the isomers, and later incorporating five potential impurities. A robust separation system was eventually developed that effectively resolves all compounds within a reasonable analysis time. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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