Crystallization and initial X-ray diffraction studies of higher plant photosystem I.
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| Title: | Crystallization and initial X-ray diffraction studies of higher plant photosystem I. |
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| Authors: | Ben-Shem, Adam1 adam@post.tau.ac.il, Nelson, Nathan1, Friolow, Felix2 |
| Source: | Acta Crystallographica: Section D (Wiley-Blackwell). Oct2003, Vol. 59 Issue 10, p1824. 4p. |
| Subjects: | Crystallization, Peas, Synchrotron radiation, Isomorphism (Crystallography), X-ray diffraction, Patterson function |
| Abstract: | Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 Å resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P2[SUB1], with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 Å, Β = 90.484°. Data sets from three crystals have been collected and were merged into a 4.4 Å resolution data set, taing advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Crystallographica: Section D (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 11070958 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Crystallization and initial X-ray diffraction studies of higher plant photosystem I. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ben-Shem%2C+Adam%22">Ben-Shem, Adam</searchLink><relatesTo>1</relatesTo><i> adam@post.tau.ac.il</i><br /><searchLink fieldCode="AR" term="%22Nelson%2C+Nathan%22">Nelson, Nathan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Friolow%2C+Felix%22">Friolow, Felix</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Crystallographica%3A+Section+D+%28Wiley-Blackwell%29%22">Acta Crystallographica: Section D (Wiley-Blackwell)</searchLink>. Oct2003, Vol. 59 Issue 10, p1824. 4p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Crystallization%22">Crystallization</searchLink><br /><searchLink fieldCode="DE" term="%22Peas%22">Peas</searchLink><br /><searchLink fieldCode="DE" term="%22Synchrotron+radiation%22">Synchrotron radiation</searchLink><br /><searchLink fieldCode="DE" term="%22Isomorphism+%28Crystallography%29%22">Isomorphism (Crystallography)</searchLink><br /><searchLink fieldCode="DE" term="%22X-ray+diffraction%22">X-ray diffraction</searchLink><br /><searchLink fieldCode="DE" term="%22Patterson+function%22">Patterson function</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 Å resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P2[SUB1], with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 Å, Β = 90.484°. Data sets from three crystals have been collected and were merged into a 4.4 Å resolution data set, taing advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Crystallographica: Section D (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1107/S0907444903016056 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 1824 Subjects: – SubjectFull: Crystallization Type: general – SubjectFull: Peas Type: general – SubjectFull: Synchrotron radiation Type: general – SubjectFull: Isomorphism (Crystallography) Type: general – SubjectFull: X-ray diffraction Type: general – SubjectFull: Patterson function Type: general Titles: – TitleFull: Crystallization and initial X-ray diffraction studies of higher plant photosystem I. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ben-Shem, Adam – PersonEntity: Name: NameFull: Nelson, Nathan – PersonEntity: Name: NameFull: Friolow, Felix IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2003 Type: published Y: 2003 Identifiers: – Type: issn-print Value: 09074449 Numbering: – Type: volume Value: 59 – Type: issue Value: 10 Titles: – TitleFull: Acta Crystallographica: Section D (Wiley-Blackwell) Type: main |
| ResultId | 1 |