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Custom fit 3D-printed brain holders for comparison of histology with MRI in marmosets.

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Title: Custom fit 3D-printed brain holders for comparison of histology with MRI in marmosets.
Authors: Guy, Joseph R.1 joseph.guy@nih.gov, Sati, Pascal1 pascal.sati@nih.gov, Leibovitch, Emily1 leibovitchel@ninds.nih.gov, Jacobson, Steven1 JacobsonS@ninds.nih.gov, Silva, Afonso C.2 SilvaA@ninds.nih.gov, Reich, Daniel S.1 reichds@ninds.nih.gov
Source: Journal of Neuroscience Methods. Jan2016, Vol. 257, p55-63. 9p.
Subjects: Magnetic resonance imaging of the brain, Histology, Comparative neurobiology, Three-dimensional printing, Diagnosis of neurological disorders
Abstract: Background MRI has the advantage of sampling large areas of tissue and locating areas of interest in 3D space in both living and ex vivo systems, whereas histology has the ability to examine thin slices of ex vivo tissue with high detail and specificity. Although both are valuable tools, it is currently difficult to make high-precision comparisons between MRI and histology due to large differences inherent to the techniques. A method combining the advantages would be an asset to understanding the pathological correlates of MRI. New method 3D-printed brain holders were used to maintain marmoset brains in the same orientation during acquisition of ex vivo MRI and pathologic cutting of the tissue. Results The results of maintaining this same orientation show that sub-millimeter, discrete neuropathological features in marmoset brain consistently share size, shape, and location between histology and ex vivo MRI, which facilitates comparison with serial imaging acquired in vivo. Comparison with existing methods Existing methods use computational approaches sensitive to data input in order to warp histologic images to match large-scale features on MRI, but the new method requires no warping of images, due to a preregistration accomplished in the technique, and is insensitive to data formatting and artifacts in both MRI and histology. Conclusions The simple method of using 3D-printed brain holders to match brain orientation during pathologic sectioning and MRI acquisition enables rapid and precise comparison of small features seen on MRI to their underlying histology. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Neuroscience Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Custom fit 3D-printed brain holders for comparison of histology with MRI in marmosets.
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  Data: <searchLink fieldCode="AR" term="%22Guy%2C+Joseph+R%2E%22">Guy, Joseph R.</searchLink><relatesTo>1</relatesTo><i> joseph.guy@nih.gov</i><br /><searchLink fieldCode="AR" term="%22Sati%2C+Pascal%22">Sati, Pascal</searchLink><relatesTo>1</relatesTo><i> pascal.sati@nih.gov</i><br /><searchLink fieldCode="AR" term="%22Leibovitch%2C+Emily%22">Leibovitch, Emily</searchLink><relatesTo>1</relatesTo><i> leibovitchel@ninds.nih.gov</i><br /><searchLink fieldCode="AR" term="%22Jacobson%2C+Steven%22">Jacobson, Steven</searchLink><relatesTo>1</relatesTo><i> JacobsonS@ninds.nih.gov</i><br /><searchLink fieldCode="AR" term="%22Silva%2C+Afonso+C%2E%22">Silva, Afonso C.</searchLink><relatesTo>2</relatesTo><i> SilvaA@ninds.nih.gov</i><br /><searchLink fieldCode="AR" term="%22Reich%2C+Daniel+S%2E%22">Reich, Daniel S.</searchLink><relatesTo>1</relatesTo><i> reichds@ninds.nih.gov</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Neuroscience+Methods%22">Journal of Neuroscience Methods</searchLink>. Jan2016, Vol. 257, p55-63. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Magnetic+resonance+imaging+of+the+brain%22">Magnetic resonance imaging of the brain</searchLink><br /><searchLink fieldCode="DE" term="%22Histology%22">Histology</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+neurobiology%22">Comparative neurobiology</searchLink><br /><searchLink fieldCode="DE" term="%22Three-dimensional+printing%22">Three-dimensional printing</searchLink><br /><searchLink fieldCode="DE" term="%22Diagnosis+of+neurological+disorders%22">Diagnosis of neurological disorders</searchLink>
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  Label: Abstract
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  Data: Background MRI has the advantage of sampling large areas of tissue and locating areas of interest in 3D space in both living and ex vivo systems, whereas histology has the ability to examine thin slices of ex vivo tissue with high detail and specificity. Although both are valuable tools, it is currently difficult to make high-precision comparisons between MRI and histology due to large differences inherent to the techniques. A method combining the advantages would be an asset to understanding the pathological correlates of MRI. New method 3D-printed brain holders were used to maintain marmoset brains in the same orientation during acquisition of ex vivo MRI and pathologic cutting of the tissue. Results The results of maintaining this same orientation show that sub-millimeter, discrete neuropathological features in marmoset brain consistently share size, shape, and location between histology and ex vivo MRI, which facilitates comparison with serial imaging acquired in vivo. Comparison with existing methods Existing methods use computational approaches sensitive to data input in order to warp histologic images to match large-scale features on MRI, but the new method requires no warping of images, due to a preregistration accomplished in the technique, and is insensitive to data formatting and artifacts in both MRI and histology. Conclusions The simple method of using 3D-printed brain holders to match brain orientation during pathologic sectioning and MRI acquisition enables rapid and precise comparison of small features seen on MRI to their underlying histology. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Neuroscience Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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