Glutathione is involved in physiological response of Candida utilis to acid stress.
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| Title: | Glutathione is involved in physiological response of Candida utilis to acid stress. |
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| Authors: | Wang, Da-Hui1, Zhang, Jun-Li1, Dong, Ying-Ying1, Wei, Gong-Yuan1 weigy@suda.edu.cn, Qi, Bin2 qibin65@126.com |
| Source: | Applied Microbiology & Biotechnology. Dec2015, Vol. 99 Issue 24, p10669-10679. 11p. |
| Subjects: | Candida utilis, Physiological effects of glutathione, Effect of acids on fungi, Fungal mutation, Catalase, Superoxide dismutase, Adenosine triphosphate |
| Abstract: | Candida utilis often encounters an acid stress environment when hexose and pentose are metabolized to produce acidic bio-based materials. In order to reveal the physiological role of glutathione (GSH) in the response of cells of this industrial yeast to acid stress, an efficient GSH-producing strain of C. utilis CCTCC M 209298 and its mutants deficient in GSH biosynthesis, C. utilis Δ gsh1 and Δ gsh2, were used in this study. A long-term mild acid challenge (pH 3.5 for 6 h) and a short-term severe acid challenge (pH 1.5 for 2 h) were conducted at 18 h during batch culture of the yeast to generate acid stress conditions. Differences in the physiological performances among the three strains under acid stress were analyzed in terms of GSH biosynthesis and distribution; intracellular pH; activities of γ-glutamylcysteine synthetase, catalase, and superoxide dismutase; intracellular ATP level; and ATP/ADP ratio. The intracellular GSH content of the yeast was found to be correlated with changes in physiological data, and a higher intracellular GSH content led to greater relief of cells to the acid stress, suggesting that GSH may be involved in protecting C. utilis against acid stress. Results presented in this manuscript not only increase our understanding of the impact of GSH on the physiology of C. utilis but also help us to comprehend the mechanism underlying the response to acid stress of eukaryotic microorganisms. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 111243335 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Glutathione is involved in physiological response of Candida utilis to acid stress. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Da-Hui%22">Wang, Da-Hui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jun-Li%22">Zhang, Jun-Li</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Dong%2C+Ying-Ying%22">Dong, Ying-Ying</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wei%2C+Gong-Yuan%22">Wei, Gong-Yuan</searchLink><relatesTo>1</relatesTo><i> weigy@suda.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Qi%2C+Bin%22">Qi, Bin</searchLink><relatesTo>2</relatesTo><i> qibin65@126.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Dec2015, Vol. 99 Issue 24, p10669-10679. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Candida+utilis%22">Candida utilis</searchLink><br /><searchLink fieldCode="DE" term="%22Physiological+effects+of+glutathione%22">Physiological effects of glutathione</searchLink><br /><searchLink fieldCode="DE" term="%22Effect+of+acids+on+fungi%22">Effect of acids on fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Fungal+mutation%22">Fungal mutation</searchLink><br /><searchLink fieldCode="DE" term="%22Catalase%22">Catalase</searchLink><br /><searchLink fieldCode="DE" term="%22Superoxide+dismutase%22">Superoxide dismutase</searchLink><br /><searchLink fieldCode="DE" term="%22Adenosine+triphosphate%22">Adenosine triphosphate</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Candida utilis often encounters an acid stress environment when hexose and pentose are metabolized to produce acidic bio-based materials. In order to reveal the physiological role of glutathione (GSH) in the response of cells of this industrial yeast to acid stress, an efficient GSH-producing strain of C. utilis CCTCC M 209298 and its mutants deficient in GSH biosynthesis, C. utilis Δ gsh1 and Δ gsh2, were used in this study. A long-term mild acid challenge (pH 3.5 for 6 h) and a short-term severe acid challenge (pH 1.5 for 2 h) were conducted at 18 h during batch culture of the yeast to generate acid stress conditions. Differences in the physiological performances among the three strains under acid stress were analyzed in terms of GSH biosynthesis and distribution; intracellular pH; activities of γ-glutamylcysteine synthetase, catalase, and superoxide dismutase; intracellular ATP level; and ATP/ADP ratio. The intracellular GSH content of the yeast was found to be correlated with changes in physiological data, and a higher intracellular GSH content led to greater relief of cells to the acid stress, suggesting that GSH may be involved in protecting C. utilis against acid stress. Results presented in this manuscript not only increase our understanding of the impact of GSH on the physiology of C. utilis but also help us to comprehend the mechanism underlying the response to acid stress of eukaryotic microorganisms. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-015-6940-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 10669 Subjects: – SubjectFull: Candida utilis Type: general – SubjectFull: Physiological effects of glutathione Type: general – SubjectFull: Effect of acids on fungi Type: general – SubjectFull: Fungal mutation Type: general – SubjectFull: Catalase Type: general – SubjectFull: Superoxide dismutase Type: general – SubjectFull: Adenosine triphosphate Type: general Titles: – TitleFull: Glutathione is involved in physiological response of Candida utilis to acid stress. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Da-Hui – PersonEntity: Name: NameFull: Zhang, Jun-Li – PersonEntity: Name: NameFull: Dong, Ying-Ying – PersonEntity: Name: NameFull: Wei, Gong-Yuan – PersonEntity: Name: NameFull: Qi, Bin IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 12 Text: Dec2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 99 – Type: issue Value: 24 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
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