Biodegradable and versatile polyethylenimine derivatives efficiently transfer DNA and siRNA into mammalian cells.
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| Title: | Biodegradable and versatile polyethylenimine derivatives efficiently transfer DNA and siRNA into mammalian cells. |
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| Authors: | Bansal, Ruby1,2, Gupta, Kailash C.1, Kumar, Pradeep1 pkumar@igib.res.in |
| Source: | Colloids & Surfaces B: Biointerfaces. Nov2015, Vol. 135, p661-668. 8p. |
| Subjects: | Polyethyleneimine, Biodegradable plastics, Chemical derivatives, Small interfering RNA, Gene delivery techniques, Methyl acrylate |
| Abstract: | Polyethylenimines (PEIs) are considered as the most promising vectors for non-viral gene delivery applications. Here, we report the synthesis and in vitro evaluation of two non-toxic and biodegradable polymers, TEPA@bPEI (TBP) and TEPA@lPEI (TLP), derived from low molecular weight branched and linear polyethylenimines by the stepwise reactions with methylacrylate (aza-Michael reaction) and amidation with tetraethylenepentamine (TEPA). These polymers not only showed their ability to bind and condense pDNA into nano-sized complexes but also provided protection against nucleases in cellular milieu. Both the polymers exhibited excellent buffering capacity and efficiently delivered nucleic acids (plasmid DNA and siRNA) across the mammalian cells (CHO and A549 cells) and outclassed native polymers and the commercial transfection reagents in terms of transfection efficiency and target gene silencing, and that too without compromising on biocompatibility i.e. toxicity. The results advocate the promising potential of the PEI derivatives as safe and potent nucleic acid carriers for practical gene delivery applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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