Whey protein mucoadhesive properties for oral drug delivery: Mucin–whey protein interaction and mucoadhesive bond strength.
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| Title: | Whey protein mucoadhesive properties for oral drug delivery: Mucin–whey protein interaction and mucoadhesive bond strength. |
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| Authors: | Hsein, Hassana1, Garrait, Ghislain1, Beyssac, Eric1 eric.beyssac@usamail.fr, Hoffart, Valérie1 |
| Source: | Colloids & Surfaces B: Biointerfaces. Dec2015, Vol. 136, p799-808. 10p. |
| Subjects: | Whey proteins, Oral medication, Bioadhesive drug delivery systems, Bond strengths, Mucins, Viscosity |
| Abstract: | Whey protein is a natural polymer recently used as an excipient in buccoadhesive tablets but its mucoadhesive properties were barely studied. In this work, we characterize mucoadhesion of whey protein in order to determine the mechanisms and optimal conditions for use as excipient in oral drug delivery. Thus, native and denatured whey protein (NWP and DWP) were investigated and the effect of concentration and pH were also studied. Many methods of characterization were selected to allow the study of chemical and physical interactions with mucin and then the results were bound with an ex vivo experiments. Turbidity of WP–mucin mixture increased at acidic pH 1.2 till 4.5 indicating interaction with mucin but not at pH 6.8. No interaction with mucin was also found by ITC method at pH 6.8 for native and denatured whey protein used at 1% (w/w). Forces of bioadhesion evaluated by viscosity measurements were the best for high concentrated (10.8%) DWP solutions at pH 6.8 and were low at pH 1.2 for NWP and DWP solutions. Addition of chemical blockers indicated that hydrogen bondings and disulfide bridges were the main mechanisms of interactions with mucin. Reticulation of DWP with calcium ions to obtain microparticles (MP) did not influence the ability of interaction with mucin as shown by FTIR analysis. These results correlated with ex vivo study on rat tissue demonstrating important adhesion (75%) of WP MP on the intestine and null on the stomach after 2 h of deposit. [ABSTRACT FROM AUTHOR] |
| Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 111877024 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Whey protein mucoadhesive properties for oral drug delivery: Mucin–whey protein interaction and mucoadhesive bond strength. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Hsein%2C+Hassana%22">Hsein, Hassana</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Garrait%2C+Ghislain%22">Garrait, Ghislain</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Beyssac%2C+Eric%22">Beyssac, Eric</searchLink><relatesTo>1</relatesTo><i> eric.beyssac@usamail.fr</i><br /><searchLink fieldCode="AR" term="%22Hoffart%2C+Valérie%22">Hoffart, Valérie</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Colloids+%26+Surfaces+B%3A+Biointerfaces%22">Colloids & Surfaces B: Biointerfaces</searchLink>. Dec2015, Vol. 136, p799-808. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Whey+proteins%22">Whey proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Oral+medication%22">Oral medication</searchLink><br /><searchLink fieldCode="DE" term="%22Bioadhesive+drug+delivery+systems%22">Bioadhesive drug delivery systems</searchLink><br /><searchLink fieldCode="DE" term="%22Bond+strengths%22">Bond strengths</searchLink><br /><searchLink fieldCode="DE" term="%22Mucins%22">Mucins</searchLink><br /><searchLink fieldCode="DE" term="%22Viscosity%22">Viscosity</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Whey protein is a natural polymer recently used as an excipient in buccoadhesive tablets but its mucoadhesive properties were barely studied. In this work, we characterize mucoadhesion of whey protein in order to determine the mechanisms and optimal conditions for use as excipient in oral drug delivery. Thus, native and denatured whey protein (NWP and DWP) were investigated and the effect of concentration and pH were also studied. Many methods of characterization were selected to allow the study of chemical and physical interactions with mucin and then the results were bound with an ex vivo experiments. Turbidity of WP–mucin mixture increased at acidic pH 1.2 till 4.5 indicating interaction with mucin but not at pH 6.8. No interaction with mucin was also found by ITC method at pH 6.8 for native and denatured whey protein used at 1% (w/w). Forces of bioadhesion evaluated by viscosity measurements were the best for high concentrated (10.8%) DWP solutions at pH 6.8 and were low at pH 1.2 for NWP and DWP solutions. Addition of chemical blockers indicated that hydrogen bondings and disulfide bridges were the main mechanisms of interactions with mucin. Reticulation of DWP with calcium ions to obtain microparticles (MP) did not influence the ability of interaction with mucin as shown by FTIR analysis. These results correlated with ex vivo study on rat tissue demonstrating important adhesion (75%) of WP MP on the intestine and null on the stomach after 2 h of deposit. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Colloids & Surfaces B: Biointerfaces is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.colsurfb.2015.10.016 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 799 Subjects: – SubjectFull: Whey proteins Type: general – SubjectFull: Oral medication Type: general – SubjectFull: Bioadhesive drug delivery systems Type: general – SubjectFull: Bond strengths Type: general – SubjectFull: Mucins Type: general – SubjectFull: Viscosity Type: general Titles: – TitleFull: Whey protein mucoadhesive properties for oral drug delivery: Mucin–whey protein interaction and mucoadhesive bond strength. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hsein, Hassana – PersonEntity: Name: NameFull: Garrait, Ghislain – PersonEntity: Name: NameFull: Beyssac, Eric – PersonEntity: Name: NameFull: Hoffart, Valérie IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 09277765 Numbering: – Type: volume Value: 136 Titles: – TitleFull: Colloids & Surfaces B: Biointerfaces Type: main |
| ResultId | 1 |
