Evaluation and comparison of the in vitro characteristics and chondrogenic capacity of four adult stem/progenitor cells for cartilage cell-based repair.
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| Title: | Evaluation and comparison of the in vitro characteristics and chondrogenic capacity of four adult stem/progenitor cells for cartilage cell-based repair. |
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| Authors: | Shafiee, Abbas1,2,3, Kabiri, Mahboubeh2,4, Langroudi, Lida2,5, Soleimani, Masoud6, Ai, Jafar1,7 |
| Source: | Journal of Biomedical Materials Research, Part A. Mar2016, Vol. 104 Issue 3, p600-610. 11p. |
| Abstract: | Cell-based therapy is being considered as a promising approach to regenerate damaged cartilage. Though, autologous chondrocyte implantation is the most effective strategy currently in use, but is hampered by some drawbacks seeking comprehensive research to surmount existing limitations or introducing alternative cell sources. In this study, we aimed to evaluate and compare the in vitro characteristics and chondrogenic capacity of some easily available adult cell sources for use in cartilage repair which includes: bone marrow-derived mesenchymal stem cells (MSC), adipose tissue-derived MSC, articular chondrocyte progenitors, and nasal septum-derived progenitors. Human stem/progenitor cells were isolated and expanded. Cell's immunophenotype, biosafety, and cell cycle status were evaluated. Also, cells were seeded onto aligned electrospun poly ( l-lactic acid)/poly ( ε-caprolactone) nanofibrous scaffolds and their proliferation rate as well as chondrogenic potential were assessed. Cells were almost phenotypically alike as they showed similar cell surface marker expression pattern. The aligned nanofibrous hybrid scaffolds could support the proliferation and chondrogenic differentiation of all cell types. However, nasal cartilage progenitors showed a higher proliferation potential and a higher chondrogenic capacity. Though, mostly similar in the majority of the studied features, nasal septum progenitors demonstrated a higher chondrogenic potential that in combination with their higher proliferation rate and easier access to the source tissue, introduces it as a promising cell source for cartilage tissue engineering and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 600-610, 2016. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biomedical Materials Research, Part A is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 112505982 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/jbm.a.35603 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 600 Titles: – TitleFull: Evaluation and comparison of the in vitro characteristics and chondrogenic capacity of four adult stem/progenitor cells for cartilage cell-based repair. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Shafiee, Abbas – PersonEntity: Name: NameFull: Kabiri, Mahboubeh – PersonEntity: Name: NameFull: Langroudi, Lida – PersonEntity: Name: NameFull: Soleimani, Masoud – PersonEntity: Name: NameFull: Ai, Jafar IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 15493296 Numbering: – Type: volume Value: 104 – Type: issue Value: 3 Titles: – TitleFull: Journal of Biomedical Materials Research, Part A Type: main |
| ResultId | 1 |
