Taxonomic analysis of the microbial community in stored sugar beets using high-throughput sequencing of different marker genes.
Saved in:
| Title: | Taxonomic analysis of the microbial community in stored sugar beets using high-throughput sequencing of different marker genes. |
|---|---|
| Authors: | Liebe, Sebastian1, Wibberg, Daniel2, Winkler, Anika2, Pühler, Alfred2, Schlüter, Andreas2, Varrelmann, Mark1 varrelmann@ifz-goettingen.de |
| Source: | FEMS Microbiology Ecology. Feb2016, Vol. 92 Issue 2, p1-12. 12p. 4 Charts, 2 Graphs. |
| Subjects: | Sugar beets, Fungal diseases of plants, Fusarium, Penicillium, Botrytis cinerea, Oomycetes |
| Abstract: | Post-harvest colonization of sugar beets accompanied by rot development is a serious problem due to sugar losses and negative impact on processing quality. Studies on the microbial community associated with rot development and factors shaping their structure are missing. Therefore, high-throughput sequencing was applied to describe the influence of environment, plant genotype and storage temperature (8°C and 20°C) on three different communities in stored sugar beets, namely fungi (internal transcribed spacers 1 and 2), Fusarium spp. (elongation factor-1α gene fragment) and oomycetes (internal transcribed spacers 1). The composition of the fungal community changed during storage mostly influenced by the storage temperature followed by a weak environmental effect. Botrytis cinerea was the prevalent species at 8°C whereas members of the fungal genera Fusarium and Penicillium became dominant at 20°C. This shift was independent of the plant genotype. Species richness within the genus Fusarium also increased during storage at both temperatures whereas the oomycetes community did not change. Moreover, oomycetes species were absent after storage at 20°C. The results of the present study clearly show that rot development during sugar beet storage is associated with pathogens well known as causal agents of post-harvest diseases in many other crops. [ABSTRACT FROM AUTHOR] |
| Copyright of FEMS Microbiology Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 113480693 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Taxonomic analysis of the microbial community in stored sugar beets using high-throughput sequencing of different marker genes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Liebe%2C+Sebastian%22">Liebe, Sebastian</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wibberg%2C+Daniel%22">Wibberg, Daniel</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Winkler%2C+Anika%22">Winkler, Anika</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Pühler%2C+Alfred%22">Pühler, Alfred</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Schlüter%2C+Andreas%22">Schlüter, Andreas</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Varrelmann%2C+Mark%22">Varrelmann, Mark</searchLink><relatesTo>1</relatesTo><i> varrelmann@ifz-goettingen.de</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22FEMS+Microbiology+Ecology%22">FEMS Microbiology Ecology</searchLink>. Feb2016, Vol. 92 Issue 2, p1-12. 12p. 4 Charts, 2 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Sugar+beets%22">Sugar beets</searchLink><br /><searchLink fieldCode="DE" term="%22Fungal+diseases+of+plants%22">Fungal diseases of plants</searchLink><br /><searchLink fieldCode="DE" term="%22Fusarium%22">Fusarium</searchLink><br /><searchLink fieldCode="DE" term="%22Penicillium%22">Penicillium</searchLink><br /><searchLink fieldCode="DE" term="%22Botrytis+cinerea%22">Botrytis cinerea</searchLink><br /><searchLink fieldCode="DE" term="%22Oomycetes%22">Oomycetes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Post-harvest colonization of sugar beets accompanied by rot development is a serious problem due to sugar losses and negative impact on processing quality. Studies on the microbial community associated with rot development and factors shaping their structure are missing. Therefore, high-throughput sequencing was applied to describe the influence of environment, plant genotype and storage temperature (8°C and 20°C) on three different communities in stored sugar beets, namely fungi (internal transcribed spacers 1 and 2), Fusarium spp. (elongation factor-1α gene fragment) and oomycetes (internal transcribed spacers 1). The composition of the fungal community changed during storage mostly influenced by the storage temperature followed by a weak environmental effect. Botrytis cinerea was the prevalent species at 8°C whereas members of the fungal genera Fusarium and Penicillium became dominant at 20°C. This shift was independent of the plant genotype. Species richness within the genus Fusarium also increased during storage at both temperatures whereas the oomycetes community did not change. Moreover, oomycetes species were absent after storage at 20°C. The results of the present study clearly show that rot development during sugar beet storage is associated with pathogens well known as causal agents of post-harvest diseases in many other crops. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of FEMS Microbiology Ecology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=113480693 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1093/femsec/fiw004 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1 Subjects: – SubjectFull: Sugar beets Type: general – SubjectFull: Fungal diseases of plants Type: general – SubjectFull: Fusarium Type: general – SubjectFull: Penicillium Type: general – SubjectFull: Botrytis cinerea Type: general – SubjectFull: Oomycetes Type: general Titles: – TitleFull: Taxonomic analysis of the microbial community in stored sugar beets using high-throughput sequencing of different marker genes. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Liebe, Sebastian – PersonEntity: Name: NameFull: Wibberg, Daniel – PersonEntity: Name: NameFull: Winkler, Anika – PersonEntity: Name: NameFull: Pühler, Alfred – PersonEntity: Name: NameFull: Schlüter, Andreas – PersonEntity: Name: NameFull: Varrelmann, Mark IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 01686496 Numbering: – Type: volume Value: 92 – Type: issue Value: 2 Titles: – TitleFull: FEMS Microbiology Ecology Type: main |
| ResultId | 1 |
