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A review on continuous-flow microfluidic PCR in droplets: Advances, challenges and future.

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Title: A review on continuous-flow microfluidic PCR in droplets: Advances, challenges and future.
Authors: Zhang, Yonghao1 yonghao.zhang@strath.ac.uk, Jiang, Hui-Rong2 huirong.jiang@strath.ac.uk
Source: Analytica Chimica Acta. Mar2016, Vol. 914, p7-16. 10p.
Subjects: Continuous flow reactors, Microfluidic devices, Polymerase chain reaction, Microdroplets, Industrial contamination prevention
Abstract: Significant advances have been made in developing microfluidic polymerase chain reaction (PCR) devices in the last two decades. More recently, microfluidic microdroplet technology has been exploited to perform PCR in droplets because of its unique features. For example, it can prevent crossover contamination and PCR inhibition, is suitable for single-cell and single-molecule analyses, and has the potential for system integration and automation. This review will therefore focus on recent developments on droplet-based continuous-flow microfluidic PCR, and the major research challenges. This paper will also discuss a new way of on-chip flow control and a rational design simulation tool, which are required to underpin fully integrated and automated droplet-based microfluidic systems. We will conclude with a scientific speculation of future autonomous scientific discoveries enabled by microfluidic microdroplet technologies. [ABSTRACT FROM AUTHOR]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Data: A review on continuous-flow microfluidic PCR in droplets: Advances, challenges and future.
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  Data: <searchLink fieldCode="DE" term="%22Continuous+flow+reactors%22">Continuous flow reactors</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidic+devices%22">Microfluidic devices</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Microdroplets%22">Microdroplets</searchLink><br /><searchLink fieldCode="DE" term="%22Industrial+contamination+prevention%22">Industrial contamination prevention</searchLink>
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  Data: Significant advances have been made in developing microfluidic polymerase chain reaction (PCR) devices in the last two decades. More recently, microfluidic microdroplet technology has been exploited to perform PCR in droplets because of its unique features. For example, it can prevent crossover contamination and PCR inhibition, is suitable for single-cell and single-molecule analyses, and has the potential for system integration and automation. This review will therefore focus on recent developments on droplet-based continuous-flow microfluidic PCR, and the major research challenges. This paper will also discuss a new way of on-chip flow control and a rational design simulation tool, which are required to underpin fully integrated and automated droplet-based microfluidic systems. We will conclude with a scientific speculation of future autonomous scientific discoveries enabled by microfluidic microdroplet technologies. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – SubjectFull: Polymerase chain reaction
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