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Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles.

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Title: Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles.
Authors: Deng, Hao-Hua1,2, Hong, Guo-Lin3, Lin, Feng-Lin1,2, Liu, Ai-Lin1,2, Xia, Xing-Hua4, Chen, Wei1,2 chenandhu@163.com
Source: Analytica Chimica Acta. Apr2016, Vol. 915, p74-80. 7p.
Subjects: Colorimetric analysis, Urease, Chemical inhibitors, Gold nanoparticles, Horseradish peroxidase, Benzidine, Complex compounds
Abstract: Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40–6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 μM and 1.8 U/L, respectively. The half-maximal inhibition value IC 50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results. [ABSTRACT FROM AUTHOR]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 113867705
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
  Group: Ti
  Data: Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles.
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  Data: <searchLink fieldCode="AR" term="%22Deng%2C+Hao-Hua%22">Deng, Hao-Hua</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Hong%2C+Guo-Lin%22">Hong, Guo-Lin</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Lin%2C+Feng-Lin%22">Lin, Feng-Lin</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Ai-Lin%22">Liu, Ai-Lin</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xia%2C+Xing-Hua%22">Xia, Xing-Hua</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Wei%22">Chen, Wei</searchLink><relatesTo>1,2</relatesTo><i> chenandhu@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Apr2016, Vol. 915, p74-80. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Colorimetric+analysis%22">Colorimetric analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Urease%22">Urease</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+inhibitors%22">Chemical inhibitors</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Horseradish+peroxidase%22">Horseradish peroxidase</searchLink><br /><searchLink fieldCode="DE" term="%22Benzidine%22">Benzidine</searchLink><br /><searchLink fieldCode="DE" term="%22Complex+compounds%22">Complex compounds</searchLink>
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  Label: Abstract
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  Data: Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3′,5,5′-tetramethylbenzidine-H 2 O 2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40–6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 μM and 1.8 U/L, respectively. The half-maximal inhibition value IC 50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1016/j.aca.2016.02.008
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 74
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      – SubjectFull: Colorimetric analysis
        Type: general
      – SubjectFull: Urease
        Type: general
      – SubjectFull: Chemical inhibitors
        Type: general
      – SubjectFull: Gold nanoparticles
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      – SubjectFull: Horseradish peroxidase
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      – SubjectFull: Benzidine
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      – SubjectFull: Complex compounds
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      – TitleFull: Colorimetric detection of urea, urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles.
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              Text: Apr2016
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