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Unique DNA-barcoded aerosol test particles for studying aerosol transport.

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Title:	Unique DNA-barcoded aerosol test particles for studying aerosol transport.
Authors:	Harding, Ruth N.¹, Hara, Christine A.¹, Hall, Sara B.¹, Vitalis, Elizabeth A.¹, Thomas, Cynthia B.¹, Jones, A. Daniel^2,3, Day, James A.⁴, Tur-Rojas, Vicente R.⁴, Jorgensen, Trond⁵, Herchert, Edwin⁵, Yoder, Richard⁵, Wheeler, Elizabeth K.¹, Farquar, George R.⁶
Source:	Aerosol Science & Technology. May2016, Vol. 50 Issue 5, p429-435. 7p.
Subjects:	Atmospheric aerosols, Genetic barcoding, Lawrence Livermore National Laboratory, DNA synthesis, Bacterial spores
Abstract:	Data are presented for the first use of novel DNA-barcoded aerosol test particles that have been developed to track the fate of airborne contaminants in populated environments. Until DNATrax (DNA Tagged Reagents for Aerosol eXperiments) particles were developed, there was no way to rapidly validate air transport models with realistic particles in the respirable range of 1–10 μm in diameter. The DNATrax particles, developed at Lawrence Livermore National Laboratory (LLNL) and tested with the assistance of the Pentagon Force Protection Agency, are the first safe and effective materials for aerosol transport studies that are identified by DNA molecules. The use of unique synthetic DNA barcodes overcomes the challenges of discerning the test material from pre-existing environmental or background contaminants (either naturally occurring or previously released). The DNATrax particle properties are demonstrated to have appropriate size range (approximately 1–4.5 μm in diameter) to accurately simulate bacterial spore transport. Here, we describe details of the first field test of the DNATrax aerosol test particles in a large indoor facility. Copyright © 2016 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	Data are presented for the first use of novel DNA-barcoded aerosol test particles that have been developed to track the fate of airborne contaminants in populated environments. Until DNATrax (DNA Tagged Reagents for Aerosol eXperiments) particles were developed, there was no way to rapidly validate air transport models with realistic particles in the respirable range of 1–10 μm in diameter. The DNATrax particles, developed at Lawrence Livermore National Laboratory (LLNL) and tested with the assistance of the Pentagon Force Protection Agency, are the first safe and effective materials for aerosol transport studies that are identified by DNA molecules. The use of unique synthetic DNA barcodes overcomes the challenges of discerning the test material from pre-existing environmental or background contaminants (either naturally occurring or previously released). The DNATrax particle properties are demonstrated to have appropriate size range (approximately 1–4.5 μm in diameter) to accurately simulate bacterial spore transport. Here, we describe details of the first field test of the DNATrax aerosol test particles in a large indoor facility. Copyright © 2016 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]
ISSN:	02786826
DOI:	10.1080/02786826.2016.1162903