View in EDS

Identification of a new operon involved in desulfurization of dibenzothiophenes using a metagenomic study and cloning and functional analysis of the genes.

Saved in:
Bibliographic Details
Title: Identification of a new operon involved in desulfurization of dibenzothiophenes using a metagenomic study and cloning and functional analysis of the genes.
Authors: Abbasian, Firouz1,2 Firouz.abbasian@gmail.com, Lockington, Robin3 Robin.Lockington@unisa.edu.au, Megharaj, Mallavarapu1,2 Megh.Mallavarapu@newcastle.edu.au, Naidu, Ravi1,2 Ravi.Naidu@newcastle.edu.au
Source: Enzyme & Microbial Technology. Jun2016, Vol. 87, p24-28. 5p.
Subjects: Operons, Desulfurization, Thiophenes, Metagenomics, Molecular cloning, Hydrocarbons, Fossil fuels
Abstract: The presence of sulphur-substituted hydrocarbons in fossil fuels are one of main reasons for the release of sulfur oxides into the environment. Dibenzothiophenes (DBT) are organic sulfur-containing molecules in crude oil, which have the potential for biological oxidation, with the sulphur being removed through an enzymatic cleavage of the C S bonds. Therefore, finding new strains that can desulfurize this compound has recently become a point of interest. In this study, three new genes involved in the bacterial desulfurization of Dibenzothiophene, which were sequenced in the course of a metagenomic study, were isolated by PCR amplification in the laboratory. The activities of these genes were then analysed following insertion into an expression vector and cloning in Escherichia coli DH5α cells. Based on the results, all three genes were actively expressed and their products could act on their corresponding substrates. [ABSTRACT FROM AUTHOR]
Copyright of Enzyme & Microbial Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:The presence of sulphur-substituted hydrocarbons in fossil fuels are one of main reasons for the release of sulfur oxides into the environment. Dibenzothiophenes (DBT) are organic sulfur-containing molecules in crude oil, which have the potential for biological oxidation, with the sulphur being removed through an enzymatic cleavage of the C S bonds. Therefore, finding new strains that can desulfurize this compound has recently become a point of interest. In this study, three new genes involved in the bacterial desulfurization of Dibenzothiophene, which were sequenced in the course of a metagenomic study, were isolated by PCR amplification in the laboratory. The activities of these genes were then analysed following insertion into an expression vector and cloning in Escherichia coli DH5α cells. Based on the results, all three genes were actively expressed and their products could act on their corresponding substrates. [ABSTRACT FROM AUTHOR]
ISSN:01410229
DOI:10.1016/j.enzmictec.2016.02.009