View in EDS

In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection

Saved in:
Bibliographic Details
Title: In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection
Authors: Aung, Hnin-Pwint1,2 hpaung@gmail.com, Pyell, Ute3
Source: Journal of Chromatography A. Jun2016, Vol. 1449, p156-165. 10p.
Subjects: Derivatization, Monosodium glutamate, Benzoic acid, Sorbic acid, Capillary electrophoresis, Canned foods, Electrolyte solutions, Separation (Technology)
Abstract: For the rapid simultaneous determination of monosodium glutamate (MSG), benzoic acid (BA), and sorbic acid (SA) in canned food and other processed food samples, we developed a method that combines in-capillary derivatization with separation by capillary electrophoresis. This method employs the rapid derivatization of MSG with o -phthalaldehyde (OPA) in the presence of 3-mercaptopropionic acid (3-MPA) and enables the detection of the resulting OPA-MSG derivative and of non-derivatized BA and SA at 230 nm. The composition of the background electrolyte and the parameters of derivatization and separation are as follows: 25 mM borax containing 5 mM OPA and 6 mM 3-MPA, separation voltage 25 mV, injection at 30 mbar for 20 s, and column temperature 25 °C. Because of the high reaction rate and suitably adapted effective electrophoretic mobilities, band broadening due to the derivatization reaction at the start of the separation process is kept to a minimum. The optimized method is validated with respect to LOD, LOQ, linearity, recovery, and precision. This method can be applied to real samples such as soy, fish, oyster and sweet and sour chili sauces after application of appropriate clean-up steps. Mechanisms of zone broadening and zone focusing are discussed showing the validity of the employed theoretical approach regarding the dependence of the peak shape for OPA-MSG on the concentration of MSG in the sample. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 115266174
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Aung%2C+Hnin-Pwint%22">Aung, Hnin-Pwint</searchLink><relatesTo>1,2</relatesTo><i> hpaung@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Pyell%2C+Ute%22">Pyell, Ute</searchLink><relatesTo>3</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Jun2016, Vol. 1449, p156-165. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Derivatization%22">Derivatization</searchLink><br /><searchLink fieldCode="DE" term="%22Monosodium+glutamate%22">Monosodium glutamate</searchLink><br /><searchLink fieldCode="DE" term="%22Benzoic+acid%22">Benzoic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Sorbic+acid%22">Sorbic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Capillary+electrophoresis%22">Capillary electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Canned+foods%22">Canned foods</searchLink><br /><searchLink fieldCode="DE" term="%22Electrolyte+solutions%22">Electrolyte solutions</searchLink><br /><searchLink fieldCode="DE" term="%22Separation+%28Technology%29%22">Separation (Technology)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: For the rapid simultaneous determination of monosodium glutamate (MSG), benzoic acid (BA), and sorbic acid (SA) in canned food and other processed food samples, we developed a method that combines in-capillary derivatization with separation by capillary electrophoresis. This method employs the rapid derivatization of MSG with o -phthalaldehyde (OPA) in the presence of 3-mercaptopropionic acid (3-MPA) and enables the detection of the resulting OPA-MSG derivative and of non-derivatized BA and SA at 230 nm. The composition of the background electrolyte and the parameters of derivatization and separation are as follows: 25 mM borax containing 5 mM OPA and 6 mM 3-MPA, separation voltage 25 mV, injection at 30 mbar for 20 s, and column temperature 25 °C. Because of the high reaction rate and suitably adapted effective electrophoretic mobilities, band broadening due to the derivatization reaction at the start of the separation process is kept to a minimum. The optimized method is validated with respect to LOD, LOQ, linearity, recovery, and precision. This method can be applied to real samples such as soy, fish, oyster and sweet and sour chili sauces after application of appropriate clean-up steps. Mechanisms of zone broadening and zone focusing are discussed showing the validity of the employed theoretical approach regarding the dependence of the peak shape for OPA-MSG on the concentration of MSG in the sample. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=115266174
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.chroma.2016.04.033
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 156
    Subjects:
      – SubjectFull: Derivatization
        Type: general
      – SubjectFull: Monosodium glutamate
        Type: general
      – SubjectFull: Benzoic acid
        Type: general
      – SubjectFull: Sorbic acid
        Type: general
      – SubjectFull: Capillary electrophoresis
        Type: general
      – SubjectFull: Canned foods
        Type: general
      – SubjectFull: Electrolyte solutions
        Type: general
      – SubjectFull: Separation (Technology)
        Type: general
    Titles:
      – TitleFull: In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Aung, Hnin-Pwint
      – PersonEntity:
          Name:
            NameFull: Pyell, Ute
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 03
              M: 06
              Text: Jun2016
              Type: published
              Y: 2016
          Identifiers:
            – Type: issn-print
              Value: 00219673
          Numbering:
            – Type: volume
              Value: 1449
          Titles:
            – TitleFull: Journal of Chromatography A
              Type: main
ResultId 1