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Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow.

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Title: Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow.
Authors: Burton, Casey1, Shi, Honglan1, Ma, Yinfa1 yinfa@mst.edu
Source: Analytica Chimica Acta. Jul2016, Vol. 927, p72-81. 10p.
Subjects: Pteridines, Liquid chromatography, Tandem mass spectrometry, Molecular epidemiology, Structural isomers
Abstract: Pteridines have evoked considerable interest from the scientific community owing to their prominent roles in human health and disease. The availability of analytical methodologies suitable for comprehensive pteridine profiling, termed here as “pterinomics”, has been limited by inconsistent sample preparation and the exclusion of lesser studied pteridine derivatives. In response, the present study describes a new pterinomics workflow using a high-performance liquid chromatography – tandem mass spectrometry (HPLC-MS/MS) methodology for the simultaneous analysis of 15 pteridine derivatives including four structural isomers, marking the largest quantitative pteridine panel that has been studied to-date. The validated method possessed excellent sensitivity with method detection limits (0.025 μg L −1 to 0.5 μg L −1 ) that were comparable or superior to existing techniques. Spiked recovery studies demonstrated the technique was both accurate (88–112%) and precise (RSD: 0–6%). A comparative study of commonly used oxidative pretreatments, including triiodide, permanganate, and manganese dioxide, revealed that the oxidative mechanisms were inefficient, complex, and concentration dependent. Finally, 50 clinical urine specimens were examined with the new technique wherein 10 pteridine derivatives were quantified and population ranges have been given. This technique can be used to examine pteridine molecular epidemiology and biochemistry to support related research applications, and may further be readily extended to include additional pteridine derivatives and biological matrices for specific applications. [ABSTRACT FROM AUTHOR]
Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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PubType: Academic Journal
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  Data: Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow.
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  Data: <searchLink fieldCode="AR" term="%22Burton%2C+Casey%22">Burton, Casey</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shi%2C+Honglan%22">Shi, Honglan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ma%2C+Yinfa%22">Ma, Yinfa</searchLink><relatesTo>1</relatesTo><i> yinfa@mst.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Jul2016, Vol. 927, p72-81. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Pteridines%22">Pteridines</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Tandem+mass+spectrometry%22">Tandem mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+epidemiology%22">Molecular epidemiology</searchLink><br /><searchLink fieldCode="DE" term="%22Structural+isomers%22">Structural isomers</searchLink>
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  Data: Pteridines have evoked considerable interest from the scientific community owing to their prominent roles in human health and disease. The availability of analytical methodologies suitable for comprehensive pteridine profiling, termed here as “pterinomics”, has been limited by inconsistent sample preparation and the exclusion of lesser studied pteridine derivatives. In response, the present study describes a new pterinomics workflow using a high-performance liquid chromatography – tandem mass spectrometry (HPLC-MS/MS) methodology for the simultaneous analysis of 15 pteridine derivatives including four structural isomers, marking the largest quantitative pteridine panel that has been studied to-date. The validated method possessed excellent sensitivity with method detection limits (0.025 μg L −1 to 0.5 μg L −1 ) that were comparable or superior to existing techniques. Spiked recovery studies demonstrated the technique was both accurate (88–112%) and precise (RSD: 0–6%). A comparative study of commonly used oxidative pretreatments, including triiodide, permanganate, and manganese dioxide, revealed that the oxidative mechanisms were inefficient, complex, and concentration dependent. Finally, 50 clinical urine specimens were examined with the new technique wherein 10 pteridine derivatives were quantified and population ranges have been given. This technique can be used to examine pteridine molecular epidemiology and biochemistry to support related research applications, and may further be readily extended to include additional pteridine derivatives and biological matrices for specific applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=115741344
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        Value: 10.1016/j.aca.2016.05.005
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        Text: English
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      – SubjectFull: Tandem mass spectrometry
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              Text: Jul2016
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