Cell proliferation and migration during early development of a symbiotic scleractinian coral.
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| Title: | Cell proliferation and migration during early development of a symbiotic scleractinian coral. |
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| Authors: | Lecointe, Agathe1,2, Domart-Coulon, Isabelle2 icoulon@mnhn.fr, Paris, Alain2, Meibom, Anders1,3 |
| Source: | Proceedings of the Royal Society B: Biological Sciences. 5/25/2016, Vol. 283 Issue 1831, p1-9. 9p. |
| Subjects: | Scleractinia, Cell proliferation, Cell migration, Dinoflagellates, Marine invertebrates -- Metamorphosis, Apoptosis |
| Abstract: | In scleractinian reef-building corals, patterns of cell self-renewal, migration and death remain virtually unknown, limiting our understanding of cellular mechanisms underlying initiation of calcification, and ontogenesis of the endosymbiotic dinoflagellate relationship. In this study, we pulse-labelled the coral Stylophora pistillata for 24 h with BrdU at four life stages (planula, early metamorphosis, primary polyp and adult colony) to investigate coral and endosymbiont cell proliferation during development, while simultaneously recording TUNEL-positive (i.e. apoptotic) nuclei. In the primary polyp, the fate of BrdU-labelled cells was tracked during a 3-day chase. The pharynx and gastrodermis were identified as the most proliferative tissues in the developing polyp, and BrdU-labelled cells accumulated in the surface pseudostratified epithelium and the skeletogenic calicodermis during the chase, revealing cell migration to these epithelia. Surprisingly, the lowest cell turnover was recorded in the calicodermis at all stages, despite active, ongoing skeletal deposition. In dinoflagellate symbionts, DNA synthesis was systematically higher than coral host gastrodermis, especially in planula and early metamorphosis. The symbiont to host cell ratio remained constant, however, indicating successive post-mitotic control mechanisms by the host of its dinoflagellate density in early life stages, increasingly shifting to apoptosis in the growing primary polyp. [ABSTRACT FROM AUTHOR] |
| Copyright of Proceedings of the Royal Society B: Biological Sciences is the property of Royal Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 116143431 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Cell proliferation and migration during early development of a symbiotic scleractinian coral. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lecointe%2C+Agathe%22">Lecointe, Agathe</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Domart-Coulon%2C+Isabelle%22">Domart-Coulon, Isabelle</searchLink><relatesTo>2</relatesTo><i> icoulon@mnhn.fr</i><br /><searchLink fieldCode="AR" term="%22Paris%2C+Alain%22">Paris, Alain</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Meibom%2C+Anders%22">Meibom, Anders</searchLink><relatesTo>1,3</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Proceedings+of+the+Royal+Society+B%3A+Biological+Sciences%22">Proceedings of the Royal Society B: Biological Sciences</searchLink>. 5/25/2016, Vol. 283 Issue 1831, p1-9. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Scleractinia%22">Scleractinia</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+migration%22">Cell migration</searchLink><br /><searchLink fieldCode="DE" term="%22Dinoflagellates%22">Dinoflagellates</searchLink><br /><searchLink fieldCode="DE" term="%22Marine+invertebrates+--+Metamorphosis%22">Marine invertebrates -- Metamorphosis</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In scleractinian reef-building corals, patterns of cell self-renewal, migration and death remain virtually unknown, limiting our understanding of cellular mechanisms underlying initiation of calcification, and ontogenesis of the endosymbiotic dinoflagellate relationship. In this study, we pulse-labelled the coral Stylophora pistillata for 24 h with BrdU at four life stages (planula, early metamorphosis, primary polyp and adult colony) to investigate coral and endosymbiont cell proliferation during development, while simultaneously recording TUNEL-positive (i.e. apoptotic) nuclei. In the primary polyp, the fate of BrdU-labelled cells was tracked during a 3-day chase. The pharynx and gastrodermis were identified as the most proliferative tissues in the developing polyp, and BrdU-labelled cells accumulated in the surface pseudostratified epithelium and the skeletogenic calicodermis during the chase, revealing cell migration to these epithelia. Surprisingly, the lowest cell turnover was recorded in the calicodermis at all stages, despite active, ongoing skeletal deposition. In dinoflagellate symbionts, DNA synthesis was systematically higher than coral host gastrodermis, especially in planula and early metamorphosis. The symbiont to host cell ratio remained constant, however, indicating successive post-mitotic control mechanisms by the host of its dinoflagellate density in early life stages, increasingly shifting to apoptosis in the growing primary polyp. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Proceedings of the Royal Society B: Biological Sciences is the property of Royal Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1098/rspb.2016.0206 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: Scleractinia Type: general – SubjectFull: Cell proliferation Type: general – SubjectFull: Cell migration Type: general – SubjectFull: Dinoflagellates Type: general – SubjectFull: Marine invertebrates -- Metamorphosis Type: general – SubjectFull: Apoptosis Type: general Titles: – TitleFull: Cell proliferation and migration during early development of a symbiotic scleractinian coral. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lecointe, Agathe – PersonEntity: Name: NameFull: Domart-Coulon, Isabelle – PersonEntity: Name: NameFull: Paris, Alain – PersonEntity: Name: NameFull: Meibom, Anders IsPartOfRelationships: – BibEntity: Dates: – D: 25 M: 05 Text: 5/25/2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 09628452 Numbering: – Type: volume Value: 283 – Type: issue Value: 1831 Titles: – TitleFull: Proceedings of the Royal Society B: Biological Sciences Type: main |
| ResultId | 1 |
