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Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.

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Title: Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
Authors: Karray, Raida1, Hamza, Manel1, Sayadi, Sami1 sami.sayadi@cbs.rnrt.tn
Source: Bioresource Technology. Sep2016, Vol. 216, p622-628. 7p.
Subjects: Biogas production, Nitrogen, Fungal enzymes, Green algae, Aspergillus niger, Ulva, Feedstock
Abstract: Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition. The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars. In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source. The cocktail is rich on β-glucosidase, pectinase and carboxy-methyl-cellulase (CMCase). The highest activity was obtained with β-glucosidase (109 IU mL −1 ) and pectinase (76 IU mL −1 ), while CMCase present the lowest activity 4.6 IU mL −1 . The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175 mL g COD int −1 . [ABSTRACT FROM AUTHOR]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 116630513
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  Data: Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
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  Data: <searchLink fieldCode="AR" term="%22Karray%2C+Raida%22">Karray, Raida</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hamza%2C+Manel%22">Hamza, Manel</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sayadi%2C+Sami%22">Sayadi, Sami</searchLink><relatesTo>1</relatesTo><i> sami.sayadi@cbs.rnrt.tn</i>
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  Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Sep2016, Vol. 216, p622-628. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Biogas+production%22">Biogas production</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrogen%22">Nitrogen</searchLink><br /><searchLink fieldCode="DE" term="%22Fungal+enzymes%22">Fungal enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Green+algae%22">Green algae</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus+niger%22">Aspergillus niger</searchLink><br /><searchLink fieldCode="DE" term="%22Ulva%22">Ulva</searchLink><br /><searchLink fieldCode="DE" term="%22Feedstock%22">Feedstock</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition. The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars. In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source. The cocktail is rich on β-glucosidase, pectinase and carboxy-methyl-cellulase (CMCase). The highest activity was obtained with β-glucosidase (109 IU mL −1 ) and pectinase (76 IU mL −1 ), while CMCase present the lowest activity 4.6 IU mL −1 . The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175 mL g COD int −1 . [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.biortech.2016.05.067
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 7
        StartPage: 622
    Subjects:
      – SubjectFull: Biogas production
        Type: general
      – SubjectFull: Nitrogen
        Type: general
      – SubjectFull: Fungal enzymes
        Type: general
      – SubjectFull: Green algae
        Type: general
      – SubjectFull: Aspergillus niger
        Type: general
      – SubjectFull: Ulva
        Type: general
      – SubjectFull: Feedstock
        Type: general
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      – TitleFull: Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
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            NameFull: Karray, Raida
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            NameFull: Hamza, Manel
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            NameFull: Sayadi, Sami
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            – D: 15
              M: 09
              Text: Sep2016
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              Value: 216
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