13C magnetic resonance spectroscopy measurements with hyperpolarized [1-13C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo.
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| Title: | 13C magnetic resonance spectroscopy measurements with hyperpolarized [1-13C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo. |
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| Authors: | Dzien, Piotr1,2, Tee, Sui‐Seng1,2, Kettunen, Mikko I.1,2, Lyons, Scott K.1,2, Larkin, Timothy J.1, Timm, Kerstin N.1, Hu, De‐En1,2, Wright, Alan2, Rodrigues, Tiago B.1,2, Serrao, Eva M.1,2, Marco‐Rius, Irene1, Mannion, Elizabeth2, D'Santos, Paula2, Kennedy, Brett W. C.1, Brindle, Kevin M.1,2 |
| Source: | Magnetic Resonance in Medicine. Aug2016, Vol. 76 Issue 2, p391-401. 11p. |
| Abstract: | Purpose Dissolution dynamic nuclear polarization can increase the sensitivity of the 13C magnetic resonance spectroscopy experiment by at least four orders of magnitude and offers a novel approach to the development of MRI gene reporters based on enzymes that metabolize 13C-labeled tracers. We describe here a gene reporter based on the enzyme pyruvate decarboxylase (EC 4.1.1.1), which catalyzes the decarboxylation of pyruvate to produce acetaldehyde and carbon dioxide. Methods Pyruvate decarboxylase from Zymomonas mobilis ( zmPDC) and a mutant that lacked enzyme activity were expressed using an inducible promoter in human embryonic kidney (HEK293T) cells. Enzyme activity was measured in the cells and in xenografts derived from the cells using 13C MRS measurements of the conversion of hyperpolarized [1-13C] pyruvate to H13 [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: <superscript>13</superscript>C magnetic resonance spectroscopy measurements with hyperpolarized [1-<superscript>13</superscript>C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dzien%2C+Piotr%22">Dzien, Piotr</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Tee%2C+Sui‐Seng%22">Tee, Sui‐Seng</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kettunen%2C+Mikko+I%2E%22">Kettunen, Mikko I.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lyons%2C+Scott+K%2E%22">Lyons, Scott K.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Larkin%2C+Timothy+J%2E%22">Larkin, Timothy J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Timm%2C+Kerstin+N%2E%22">Timm, Kerstin N.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hu%2C+De‐En%22">Hu, De‐En</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wright%2C+Alan%22">Wright, Alan</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Rodrigues%2C+Tiago+B%2E%22">Rodrigues, Tiago B.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Serrao%2C+Eva+M%2E%22">Serrao, Eva M.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Marco‐Rius%2C+Irene%22">Marco‐Rius, Irene</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mannion%2C+Elizabeth%22">Mannion, Elizabeth</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22D'Santos%2C+Paula%22">D'Santos, Paula</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kennedy%2C+Brett+W%2E+C%2E%22">Kennedy, Brett W. C.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Brindle%2C+Kevin+M%2E%22">Brindle, Kevin M.</searchLink><relatesTo>1,2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Aug2016, Vol. 76 Issue 2, p391-401. 11p. – Name: Abstract Label: Abstract Group: Ab Data: Purpose Dissolution dynamic nuclear polarization can increase the sensitivity of the 13C magnetic resonance spectroscopy experiment by at least four orders of magnitude and offers a novel approach to the development of MRI gene reporters based on enzymes that metabolize 13C-labeled tracers. We describe here a gene reporter based on the enzyme pyruvate decarboxylase (EC 4.1.1.1), which catalyzes the decarboxylation of pyruvate to produce acetaldehyde and carbon dioxide. Methods Pyruvate decarboxylase from Zymomonas mobilis ( zmPDC) and a mutant that lacked enzyme activity were expressed using an inducible promoter in human embryonic kidney (HEK293T) cells. Enzyme activity was measured in the cells and in xenografts derived from the cells using 13C MRS measurements of the conversion of hyperpolarized [1-13C] pyruvate to H13 [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.25879 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 391 Titles: – TitleFull: 13C magnetic resonance spectroscopy measurements with hyperpolarized [1-13C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dzien, Piotr – PersonEntity: Name: NameFull: Tee, Sui‐Seng – PersonEntity: Name: NameFull: Kettunen, Mikko I. – PersonEntity: Name: NameFull: Lyons, Scott K. – PersonEntity: Name: NameFull: Larkin, Timothy J. – PersonEntity: Name: NameFull: Timm, Kerstin N. – PersonEntity: Name: NameFull: Hu, De‐En – PersonEntity: Name: NameFull: Wright, Alan – PersonEntity: Name: NameFull: Rodrigues, Tiago B. – PersonEntity: Name: NameFull: Serrao, Eva M. – PersonEntity: Name: NameFull: Marco‐Rius, Irene – PersonEntity: Name: NameFull: Mannion, Elizabeth – PersonEntity: Name: NameFull: D'Santos, Paula – PersonEntity: Name: NameFull: Kennedy, Brett W. C. – PersonEntity: Name: NameFull: Brindle, Kevin M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 76 – Type: issue Value: 2 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
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