View in EDS

Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate.

Saved in:
Bibliographic Details
Title: Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate.
Authors: Du, Jia Yan1, Chen, Lan Rong2, Liu, Su1, Lin, Jiang Hui1, Liang, Qun Tao1, Lyon, Malcolm1, Wei, Zheng1 zheng0wei@hotmail.com
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Aug2016, Vol. 1028, p71-76. 6p.
Subjects: Heparan sulfate, Glucosamine, Disaccharides, Liquid chromatography-mass spectrometry, Structural analysis (Science), Pathological physiology
Abstract: The rare N -unsubstituted glucosamine (GlcNH 3 + ) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N -unsubstituted disaccharides of heparin/HS is described. Four N -unsubstituted disaccharides, together with eight N -substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH 3 + residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH 3 + residues and their increasingly important function in human health and disease. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Description
Abstract:The rare N -unsubstituted glucosamine (GlcNH 3 + ) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N -unsubstituted disaccharides of heparin/HS is described. Four N -unsubstituted disaccharides, together with eight N -substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH 3 + residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH 3 + residues and their increasingly important function in human health and disease. [ABSTRACT FROM AUTHOR]
ISSN:15700232
DOI:10.1016/j.jchromb.2016.06.006