Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate.
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| Title: | Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate. |
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| Authors: | Du, Jia Yan1, Chen, Lan Rong2, Liu, Su1, Lin, Jiang Hui1, Liang, Qun Tao1, Lyon, Malcolm1, Wei, Zheng1 zheng0wei@hotmail.com |
| Source: | Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Aug2016, Vol. 1028, p71-76. 6p. |
| Subjects: | Heparan sulfate, Glucosamine, Disaccharides, Liquid chromatography-mass spectrometry, Structural analysis (Science), Pathological physiology |
| Abstract: | The rare N -unsubstituted glucosamine (GlcNH 3 + ) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N -unsubstituted disaccharides of heparin/HS is described. Four N -unsubstituted disaccharides, together with eight N -substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH 3 + residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH 3 + residues and their increasingly important function in human health and disease. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 116864974 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Du%2C+Jia+Yan%22">Du, Jia Yan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Lan+Rong%22">Chen, Lan Rong</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Su%22">Liu, Su</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lin%2C+Jiang+Hui%22">Lin, Jiang Hui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liang%2C+Qun+Tao%22">Liang, Qun Tao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lyon%2C+Malcolm%22">Lyon, Malcolm</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wei%2C+Zheng%22">Wei, Zheng</searchLink><relatesTo>1</relatesTo><i> zheng0wei@hotmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Aug2016, Vol. 1028, p71-76. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Heparan+sulfate%22">Heparan sulfate</searchLink><br /><searchLink fieldCode="DE" term="%22Glucosamine%22">Glucosamine</searchLink><br /><searchLink fieldCode="DE" term="%22Disaccharides%22">Disaccharides</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography-mass+spectrometry%22">Liquid chromatography-mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Structural+analysis+%28Science%29%22">Structural analysis (Science)</searchLink><br /><searchLink fieldCode="DE" term="%22Pathological+physiology%22">Pathological physiology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The rare N -unsubstituted glucosamine (GlcNH 3 + ) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N -unsubstituted disaccharides of heparin/HS is described. Four N -unsubstituted disaccharides, together with eight N -substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH 3 + residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH 3 + residues and their increasingly important function in human health and disease. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jchromb.2016.06.006 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 71 Subjects: – SubjectFull: Heparan sulfate Type: general – SubjectFull: Glucosamine Type: general – SubjectFull: Disaccharides Type: general – SubjectFull: Liquid chromatography-mass spectrometry Type: general – SubjectFull: Structural analysis (Science) Type: general – SubjectFull: Pathological physiology Type: general Titles: – TitleFull: Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Du, Jia Yan – PersonEntity: Name: NameFull: Chen, Lan Rong – PersonEntity: Name: NameFull: Liu, Su – PersonEntity: Name: NameFull: Lin, Jiang Hui – PersonEntity: Name: NameFull: Liang, Qun Tao – PersonEntity: Name: NameFull: Lyon, Malcolm – PersonEntity: Name: NameFull: Wei, Zheng IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 08 Text: Aug2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 15700232 Numbering: – Type: volume Value: 1028 Titles: – TitleFull: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences Type: main |
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