Tobacco Etch Virus protease: A shortcut across biotechnologies.
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| Title: | Tobacco Etch Virus protease: A shortcut across biotechnologies. |
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| Authors: | Cesaratto, Francesca1, Burrone, Oscar R.1 burrone@icgeb.org, Petris, Gianluca2 gianluca.petris@unitn.it |
| Source: | Journal of Biotechnology. Aug2016, Vol. 231, p239-249. 11p. |
| Subjects: | Tobacco etch virus, Proteolytic enzymes, Endopeptidases, Protein engineering |
| Abstract: | About thirty years ago, studies on the RNA genome of Tobacco Etch Virus revealed the presence of an efficient and specific protease, called Tobacco Etch Virus protease (TEVp), that was part of the Nuclear Inclusion a (NIa) enzyme. TEVp is an efficient and specific protease of 27 kDa that has become a valuable biotechnological tool. Nowadays TEVp is a unique endopeptidase largely exploited in biotechnology from industrial applications to in vitro and in vivo cellular studies. A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported. Similarly, a panel of different target cleavage sites, derived from the canonical ENLYFQ-G/S site, has been established. In this review we describe these aspects of TEVp and some of its multiple applications. A particular focus is on the use and molecular biology of TEVp in living cells and organisms. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 117011016 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Tobacco Etch Virus protease: A shortcut across biotechnologies. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Cesaratto%2C+Francesca%22">Cesaratto, Francesca</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Burrone%2C+Oscar+R%2E%22">Burrone, Oscar R.</searchLink><relatesTo>1</relatesTo><i> burrone@icgeb.org</i><br /><searchLink fieldCode="AR" term="%22Petris%2C+Gianluca%22">Petris, Gianluca</searchLink><relatesTo>2</relatesTo><i> gianluca.petris@unitn.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biotechnology%22">Journal of Biotechnology</searchLink>. Aug2016, Vol. 231, p239-249. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Tobacco+etch+virus%22">Tobacco etch virus</searchLink><br /><searchLink fieldCode="DE" term="%22Proteolytic+enzymes%22">Proteolytic enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Endopeptidases%22">Endopeptidases</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+engineering%22">Protein engineering</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: About thirty years ago, studies on the RNA genome of Tobacco Etch Virus revealed the presence of an efficient and specific protease, called Tobacco Etch Virus protease (TEVp), that was part of the Nuclear Inclusion a (NIa) enzyme. TEVp is an efficient and specific protease of 27 kDa that has become a valuable biotechnological tool. Nowadays TEVp is a unique endopeptidase largely exploited in biotechnology from industrial applications to in vitro and in vivo cellular studies. A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported. Similarly, a panel of different target cleavage sites, derived from the canonical ENLYFQ-G/S site, has been established. In this review we describe these aspects of TEVp and some of its multiple applications. A particular focus is on the use and molecular biology of TEVp in living cells and organisms. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=117011016 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jbiotec.2016.06.012 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 239 Subjects: – SubjectFull: Tobacco etch virus Type: general – SubjectFull: Proteolytic enzymes Type: general – SubjectFull: Endopeptidases Type: general – SubjectFull: Protein engineering Type: general Titles: – TitleFull: Tobacco Etch Virus protease: A shortcut across biotechnologies. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Cesaratto, Francesca – PersonEntity: Name: NameFull: Burrone, Oscar R. – PersonEntity: Name: NameFull: Petris, Gianluca IsPartOfRelationships: – BibEntity: Dates: – D: 10 M: 08 Text: Aug2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 01681656 Numbering: – Type: volume Value: 231 Titles: – TitleFull: Journal of Biotechnology Type: main |
| ResultId | 1 |
