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Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma.

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Title: Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma.
Authors: Wang, Gong1 wanggong127@163.com, Zhang, Qian2 xqsnzq@163.com, Zhuo, Zhongxiong1 zzx64120@163.com, Wu, Shengzheng1 wanggong127@hotmail.com, Liu, Zheng1 wangg5@uw.edu, Xia, Hongmei1 550789302@qq.com, Tan, Kaibin1 546217589@qq.com, Zou, Linru1 xqgaoyh@gmail.com, Gan, Ling1 xqgaoyh@hotmail.com, Gao, Yunhua1 xqgaoyh@outlook.com
Source: European Radiology. Sep2016, Vol. 26 Issue 9, p3006-3016. 11p. 4 Color Photographs, 2 Black and White Photographs, 1 Chart, 1 Graph.
Subjects: Mesenchymal stem cells, Kidney disease treatments, Fluorescent proteins, Diagnostic ultrasonic imaging, Microbubble diagnosis, Therapeutics, Kidney physiology, Connective tissue cells, Animal experimentation, Bone marrow transplantation, Enzyme-linked immunosorbent assay, Microscopy, Rats, Ultrasonic imaging, Western immunoblotting, Equipment & supplies, Physiology
Abstract: Objectives: Bone marrow stromal cells (BMSC) transplantation proves successful in treating kidney disease and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explored the mechanical effects of ultrasound targeted microbubble destruction (UTMD) on BMSCs homing ability in treated kidney tissues.Methods: Rats were injected with red fluorescent protein (RFP)-labelled BMSCs and sonicated with microbubble-mediated ultrasound. Then, we tested kidney micro-environment changes induced and their influence on stem cell homing ability.Results: The results showed that the mechanical effects of UTMD would increase local and transient levels of chemoattractants (i.e. cytokines, integrins and growth factors) in targeted kidney tissues. Transmission electron microscopy showed that vascular endothelial cell was discontinuous in the UTMD group post-treatment, becoming smooth 72 h later. Confocal laser scanning microscopy and RT-PCR showed up to eight times more stem cells in the peritubular regions of experimental kidneys on days 1 and 3 post-treatment compared with the contralateral kidney.Conclusions: These results confirmed that renal micro-environment changes caused by appropriate UTMD may promote BMSC homing ability toward treated kidney tissues without renal toxicity and cell damage.Key Points: • This experiment showed a feasible strategy in promoting stem cell homing ability. • The treatment uses diagnostic ultrasound during enhancement with IV microbubbles. • A suitable micro-environment was important for targeted stem cell homing and retention. • The method is effective for stem cell homing to kidney diseases. • More work is required with larger animals before potential human trials. [ABSTRACT FROM AUTHOR]
Copyright of European Radiology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma.
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  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Gong%22">Wang, Gong</searchLink><relatesTo>1</relatesTo><i> wanggong127@163.com</i><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Qian%22">Zhang, Qian</searchLink><relatesTo>2</relatesTo><i> xqsnzq@163.com</i><br /><searchLink fieldCode="AR" term="%22Zhuo%2C+Zhongxiong%22">Zhuo, Zhongxiong</searchLink><relatesTo>1</relatesTo><i> zzx64120@163.com</i><br /><searchLink fieldCode="AR" term="%22Wu%2C+Shengzheng%22">Wu, Shengzheng</searchLink><relatesTo>1</relatesTo><i> wanggong127@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Zheng%22">Liu, Zheng</searchLink><relatesTo>1</relatesTo><i> wangg5@uw.edu</i><br /><searchLink fieldCode="AR" term="%22Xia%2C+Hongmei%22">Xia, Hongmei</searchLink><relatesTo>1</relatesTo><i> 550789302@qq.com</i><br /><searchLink fieldCode="AR" term="%22Tan%2C+Kaibin%22">Tan, Kaibin</searchLink><relatesTo>1</relatesTo><i> 546217589@qq.com</i><br /><searchLink fieldCode="AR" term="%22Zou%2C+Linru%22">Zou, Linru</searchLink><relatesTo>1</relatesTo><i> xqgaoyh@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Gan%2C+Ling%22">Gan, Ling</searchLink><relatesTo>1</relatesTo><i> xqgaoyh@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Gao%2C+Yunhua%22">Gao, Yunhua</searchLink><relatesTo>1</relatesTo><i> xqgaoyh@outlook.com</i>
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  Data: <searchLink fieldCode="JN" term="%22European+Radiology%22">European Radiology</searchLink>. Sep2016, Vol. 26 Issue 9, p3006-3016. 11p. 4 Color Photographs, 2 Black and White Photographs, 1 Chart, 1 Graph.
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  Data: <searchLink fieldCode="DE" term="%22Mesenchymal+stem+cells%22">Mesenchymal stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Kidney+disease+treatments%22">Kidney disease treatments</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescent+proteins%22">Fluorescent proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Diagnostic+ultrasonic+imaging%22">Diagnostic ultrasonic imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Microbubble+diagnosis%22">Microbubble diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Therapeutics%22">Therapeutics</searchLink><br /><searchLink fieldCode="DE" term="%22Kidney+physiology%22">Kidney physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Connective+tissue+cells%22">Connective tissue cells</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Bone+marrow+transplantation%22">Bone marrow transplantation</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink><br /><searchLink fieldCode="DE" term="%22Microscopy%22">Microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Ultrasonic+imaging%22">Ultrasonic imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Western+immunoblotting%22">Western immunoblotting</searchLink><br /><searchLink fieldCode="DE" term="%22Equipment+%26+supplies%22">Equipment & supplies</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: <bold>Objectives: </bold>Bone marrow stromal cells (BMSC) transplantation proves successful in treating kidney disease and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explored the mechanical effects of ultrasound targeted microbubble destruction (UTMD) on BMSCs homing ability in treated kidney tissues.<bold>Methods: </bold>Rats were injected with red fluorescent protein (RFP)-labelled BMSCs and sonicated with microbubble-mediated ultrasound. Then, we tested kidney micro-environment changes induced and their influence on stem cell homing ability.<bold>Results: </bold>The results showed that the mechanical effects of UTMD would increase local and transient levels of chemoattractants (i.e. cytokines, integrins and growth factors) in targeted kidney tissues. Transmission electron microscopy showed that vascular endothelial cell was discontinuous in the UTMD group post-treatment, becoming smooth 72 h later. Confocal laser scanning microscopy and RT-PCR showed up to eight times more stem cells in the peritubular regions of experimental kidneys on days 1 and 3 post-treatment compared with the contralateral kidney.<bold>Conclusions: </bold>These results confirmed that renal micro-environment changes caused by appropriate UTMD may promote BMSC homing ability toward treated kidney tissues without renal toxicity and cell damage.<bold>Key Points: </bold>• This experiment showed a feasible strategy in promoting stem cell homing ability. • The treatment uses diagnostic ultrasound during enhancement with IV microbubbles. • A suitable micro-environment was important for targeted stem cell homing and retention. • The method is effective for stem cell homing to kidney diseases. • More work is required with larger animals before potential human trials. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of European Radiology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00330-015-4123-3
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        Text: English
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      – SubjectFull: Mesenchymal stem cells
        Type: general
      – SubjectFull: Kidney disease treatments
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      – SubjectFull: Fluorescent proteins
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      – SubjectFull: Diagnostic ultrasonic imaging
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      – SubjectFull: Therapeutics
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      – SubjectFull: Kidney physiology
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      – SubjectFull: Connective tissue cells
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      – SubjectFull: Animal experimentation
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      – SubjectFull: Bone marrow transplantation
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      – SubjectFull: Enzyme-linked immunosorbent assay
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      – SubjectFull: Microscopy
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      – TitleFull: Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma.
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              M: 09
              Text: Sep2016
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