Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (A nthonomus grandis)
Saved in:
| Title: | Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (A nthonomus grandis) |
|---|---|
| Authors: | Franco, Octávio L.1,2,3, Dias, Simoni C.1,2, Magalhães, Claudio P.4, Monteiro, Ana C. S.1, Bloch Jr, Carlos1, Melo, Francislete R.2,5, Oliveira-Neto, Osmundo B.1,2, Monnerat, Rose G.1, Grossi-de-Sá, Maria Fátima1 fatimasa@cenargen.embrapa.br |
| Source: | Phytochemistry. Jan2004, Vol. 65 Issue 1, p81. 9p. |
| Subjects: | Soybean, Boll weevil, Chymotrypsin |
| Abstract: | The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 μM. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. Bioassays suggested that SKTI could be used as a tool in engineering crop plants, which might exhibit increased resistance against cotton boll weevil. [Copyright &y& Elsevier] |
| Copyright of Phytochemistry is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 11831941 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (A nthonomus grandis) – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Franco%2C+Octávio+L%2E%22">Franco, Octávio L.</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Dias%2C+Simoni+C%2E%22">Dias, Simoni C.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Magalhães%2C+Claudio+P%2E%22">Magalhães, Claudio P.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Monteiro%2C+Ana+C%2E+S%2E%22">Monteiro, Ana C. S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bloch+Jr%2C+Carlos%22">Bloch Jr, Carlos</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Melo%2C+Francislete+R%2E%22">Melo, Francislete R.</searchLink><relatesTo>2,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Oliveira-Neto%2C+Osmundo+B%2E%22">Oliveira-Neto, Osmundo B.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Monnerat%2C+Rose+G%2E%22">Monnerat, Rose G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Grossi-de-Sá%2C+Maria+Fátima%22">Grossi-de-Sá, Maria Fátima</searchLink><relatesTo>1</relatesTo><i> fatimasa@cenargen.embrapa.br</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Phytochemistry%22">Phytochemistry</searchLink>. Jan2004, Vol. 65 Issue 1, p81. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Soybean%22">Soybean</searchLink><br /><searchLink fieldCode="DE" term="%22Boll+weevil%22">Boll weevil</searchLink><br /><searchLink fieldCode="DE" term="%22Chymotrypsin%22">Chymotrypsin</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 μM. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. Bioassays suggested that SKTI could be used as a tool in engineering crop plants, which might exhibit increased resistance against cotton boll weevil. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Phytochemistry is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=11831941 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.phytochem.2003.09.010 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 81 Subjects: – SubjectFull: Soybean Type: general – SubjectFull: Boll weevil Type: general – SubjectFull: Chymotrypsin Type: general Titles: – TitleFull: Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (A nthonomus grandis) Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Franco, Octávio L. – PersonEntity: Name: NameFull: Dias, Simoni C. – PersonEntity: Name: NameFull: Magalhães, Claudio P. – PersonEntity: Name: NameFull: Monteiro, Ana C. S. – PersonEntity: Name: NameFull: Bloch Jr, Carlos – PersonEntity: Name: NameFull: Melo, Francislete R. – PersonEntity: Name: NameFull: Oliveira-Neto, Osmundo B. – PersonEntity: Name: NameFull: Monnerat, Rose G. – PersonEntity: Name: NameFull: Grossi-de-Sá, Maria Fátima IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2004 Type: published Y: 2004 Identifiers: – Type: issn-print Value: 00319422 Numbering: – Type: volume Value: 65 – Type: issue Value: 1 Titles: – TitleFull: Phytochemistry Type: main |
| ResultId | 1 |
