View in EDS

Comparison of liquid and supercritical fluid chromatography mobile phases for enantioselective separations on polysaccharide stationary phases.

Saved in:
Bibliographic Details
Title: Comparison of liquid and supercritical fluid chromatography mobile phases for enantioselective separations on polysaccharide stationary phases.
Authors: Khater, Syame1, Lozac’h, Marie-Anne2, Adam, Isabelle2, Francotte, Eric2, West, Caroline1 west_caroline@yahoo.fr
Source: Journal of Chromatography A. Oct2016, Vol. 1467, p463-472. 10p.
Subjects: Supercritical fluid chromatography, Polysaccharides, Mobile phase (Chromatography), High performance liquid chromatography, Isopropyl alcohol
Abstract: Analysis and production of enantiomerically pure compounds is a major topic of interest when active pharmaceutical ingredients are concerned. Enantioselective chromatography has become a favourite both at the analytical and preparative scales. High-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) are dominating the scene and are often seen as complementary techniques. Nowadays, for economic and ecologic reasons, SFC may be preferred over normal-phase HPLC (NPLC) as it allows significant reductions in solvent consumption. However, the transfer of NPLC methods to SFC is not always straightforward. In this study, we compare the retention of achiral molecules and separation of enantiomers under supercritical fluid (carbon dioxide with ethanol or isopropanol) and liquid normal-phase (heptane with ethanol or isopropanol) elution modes with polysaccharide stationary phases in order to explore the differences between the retention and enantioseparation properties between the two modes. Chemometric methods (namely quantitative structure-retention relationships and discriminant analysis) are employed to compare the results obtained on a large set of analytes (171 achiral probes and 97 racemates) and gain some understanding on the retention and separation mechanisms. The results indicate that, contrary to popular belief, carbon dioxide − solvent SFC mobile phases are often weaker eluents than liquid mobile phases. It appears that SFC and NPLC elution modes provide different retention mechanisms. While some enantioseparations are unaffected, facilitating the transfer between the two elution modes, other enantioseparations may be drastically different due to different types and strength of interactions contributing to enantioselectivity. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 118422555
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Comparison of liquid and supercritical fluid chromatography mobile phases for enantioselective separations on polysaccharide stationary phases.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Khater%2C+Syame%22">Khater, Syame</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lozac’h%2C+Marie-Anne%22">Lozac’h, Marie-Anne</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Adam%2C+Isabelle%22">Adam, Isabelle</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Francotte%2C+Eric%22">Francotte, Eric</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22West%2C+Caroline%22">West, Caroline</searchLink><relatesTo>1</relatesTo><i> west_caroline@yahoo.fr</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Oct2016, Vol. 1467, p463-472. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Supercritical+fluid+chromatography%22">Supercritical fluid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Polysaccharides%22">Polysaccharides</searchLink><br /><searchLink fieldCode="DE" term="%22Mobile+phase+%28Chromatography%29%22">Mobile phase (Chromatography)</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Isopropyl+alcohol%22">Isopropyl alcohol</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Analysis and production of enantiomerically pure compounds is a major topic of interest when active pharmaceutical ingredients are concerned. Enantioselective chromatography has become a favourite both at the analytical and preparative scales. High-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) are dominating the scene and are often seen as complementary techniques. Nowadays, for economic and ecologic reasons, SFC may be preferred over normal-phase HPLC (NPLC) as it allows significant reductions in solvent consumption. However, the transfer of NPLC methods to SFC is not always straightforward. In this study, we compare the retention of achiral molecules and separation of enantiomers under supercritical fluid (carbon dioxide with ethanol or isopropanol) and liquid normal-phase (heptane with ethanol or isopropanol) elution modes with polysaccharide stationary phases in order to explore the differences between the retention and enantioseparation properties between the two modes. Chemometric methods (namely quantitative structure-retention relationships and discriminant analysis) are employed to compare the results obtained on a large set of analytes (171 achiral probes and 97 racemates) and gain some understanding on the retention and separation mechanisms. The results indicate that, contrary to popular belief, carbon dioxide − solvent SFC mobile phases are often weaker eluents than liquid mobile phases. It appears that SFC and NPLC elution modes provide different retention mechanisms. While some enantioseparations are unaffected, facilitating the transfer between the two elution modes, other enantioseparations may be drastically different due to different types and strength of interactions contributing to enantioselectivity. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=118422555
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.chroma.2016.06.060
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 463
    Subjects:
      – SubjectFull: Supercritical fluid chromatography
        Type: general
      – SubjectFull: Polysaccharides
        Type: general
      – SubjectFull: Mobile phase (Chromatography)
        Type: general
      – SubjectFull: High performance liquid chromatography
        Type: general
      – SubjectFull: Isopropyl alcohol
        Type: general
    Titles:
      – TitleFull: Comparison of liquid and supercritical fluid chromatography mobile phases for enantioselective separations on polysaccharide stationary phases.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Khater, Syame
      – PersonEntity:
          Name:
            NameFull: Lozac’h, Marie-Anne
      – PersonEntity:
          Name:
            NameFull: Adam, Isabelle
      – PersonEntity:
          Name:
            NameFull: Francotte, Eric
      – PersonEntity:
          Name:
            NameFull: West, Caroline
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 07
              M: 10
              Text: Oct2016
              Type: published
              Y: 2016
          Identifiers:
            – Type: issn-print
              Value: 00219673
          Numbering:
            – Type: volume
              Value: 1467
          Titles:
            – TitleFull: Journal of Chromatography A
              Type: main
ResultId 1