Aptamer biosensor for Salmonella typhimurium detection based on luminescence energy transfer from Mn2 +-doped NaYF4:Yb, Tm upconverting nanoparticles to gold nanorods.
Saved in:
| Title: | Aptamer biosensor for Salmonella typhimurium detection based on luminescence energy transfer from Mn2 +-doped NaYF4:Yb, Tm upconverting nanoparticles to gold nanorods. |
|---|---|
| Authors: | Cheng, Keyi1, Zhang, Jianguo1, Zhang, Liping1, Wang, Lun1 wanglun@mail.ahnu.edu.cn, Chen, Hongqi1 hq80chen@mail.ahnu.edu.cn |
| Source: | Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Jan2017, Vol. 171, p168-173. 6p. |
| Subjects: | Biosensors, Salmonella typhimurium, Luminescence, Energy transfer, Gold nanoparticles |
| Abstract: | A highly sensitive luminescent bioassay for the detection of Salmonella typhimurium was fabricated using Mn 2 + -doped NaYF 4 :Yb,Tm upconversion nanoparticles (UCNPs) as the donor and gold nanorods (Au NRs) as the acceptor and utilizing an energy transfer (LET) system. Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs with a strong emission peak at 807 nm were obtained by changing the doped ion ratio. Carboxyl-terminated Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs were coupled with S. typhimurium aptamers, which were employed to capture and concentrate S. typhimurium . The electrostatic interactions shorten the distance between the negatively charged donor and the positively charged acceptor, which results in luminescence quenching. The added S. typhimurium leads to the restoration of luminescence due to the formation of UCNPs-aptamers- S. typhimurium , which repels the UCNPs-aptamers from the Au NRs. The LET system does not occur because of the nonexistence of the luminescence emission band of Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs, which had large spectral overlap with the absorption band of Au NRs. Under optimal conditions, the linear range of detecting S. typhimurium was 12 to 5 × 10 5 cfu/mL (R = 0.99). The limit of detection for S. typhimurium was as low as 11 cfu/mL in an aqueous buffer. The measurement of S. typhimurium in milk samples was satisfied in accordance with the plate-counting method, suggesting that the proposed method was of practical value in the application of food security. [ABSTRACT FROM AUTHOR] |
| Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 118470232 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Aptamer biosensor for Salmonella typhimurium detection based on luminescence energy transfer from Mn2 +-doped NaYF4:Yb, Tm upconverting nanoparticles to gold nanorods. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Cheng%2C+Keyi%22">Cheng, Keyi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jianguo%22">Zhang, Jianguo</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Liping%22">Zhang, Liping</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Lun%22">Wang, Lun</searchLink><relatesTo>1</relatesTo><i> wanglun@mail.ahnu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Hongqi%22">Chen, Hongqi</searchLink><relatesTo>1</relatesTo><i> hq80chen@mail.ahnu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. Jan2017, Vol. 171, p168-173. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Salmonella+typhimurium%22">Salmonella typhimurium</searchLink><br /><searchLink fieldCode="DE" term="%22Luminescence%22">Luminescence</searchLink><br /><searchLink fieldCode="DE" term="%22Energy+transfer%22">Energy transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A highly sensitive luminescent bioassay for the detection of Salmonella typhimurium was fabricated using Mn 2 + -doped NaYF 4 :Yb,Tm upconversion nanoparticles (UCNPs) as the donor and gold nanorods (Au NRs) as the acceptor and utilizing an energy transfer (LET) system. Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs with a strong emission peak at 807 nm were obtained by changing the doped ion ratio. Carboxyl-terminated Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs were coupled with S. typhimurium aptamers, which were employed to capture and concentrate S. typhimurium . The electrostatic interactions shorten the distance between the negatively charged donor and the positively charged acceptor, which results in luminescence quenching. The added S. typhimurium leads to the restoration of luminescence due to the formation of UCNPs-aptamers- S. typhimurium , which repels the UCNPs-aptamers from the Au NRs. The LET system does not occur because of the nonexistence of the luminescence emission band of Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs, which had large spectral overlap with the absorption band of Au NRs. Under optimal conditions, the linear range of detecting S. typhimurium was 12 to 5 × 10 5 cfu/mL (R = 0.99). The limit of detection for S. typhimurium was as low as 11 cfu/mL in an aqueous buffer. The measurement of S. typhimurium in milk samples was satisfied in accordance with the plate-counting method, suggesting that the proposed method was of practical value in the application of food security. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=118470232 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.saa.2016.08.012 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 168 Subjects: – SubjectFull: Biosensors Type: general – SubjectFull: Salmonella typhimurium Type: general – SubjectFull: Luminescence Type: general – SubjectFull: Energy transfer Type: general – SubjectFull: Gold nanoparticles Type: general Titles: – TitleFull: Aptamer biosensor for Salmonella typhimurium detection based on luminescence energy transfer from Mn2 +-doped NaYF4:Yb, Tm upconverting nanoparticles to gold nanorods. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Cheng, Keyi – PersonEntity: Name: NameFull: Zhang, Jianguo – PersonEntity: Name: NameFull: Zhang, Liping – PersonEntity: Name: NameFull: Wang, Lun – PersonEntity: Name: NameFull: Chen, Hongqi IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 01 Text: Jan2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 13861425 Numbering: – Type: volume Value: 171 Titles: – TitleFull: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy Type: main |
| ResultId | 1 |
