Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738.
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| Title: | Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738. |
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| Authors: | Sachdeva, Neha1, Gupta, Ravi Prakash2, Mathur, Anshu Shankar2 mathuras@indianoil.in, Tuli, Deepak Kumar1 |
| Source: | Bioresource Technology. Dec2016, Vol. 221, p576-587. 12p. |
| Subjects: | Lipids, Chlorella pyrenoidosa, Ethyl methanesulfonate, Mutagenesis, Biodiesel fuels |
| Abstract: | The present study aimed to develop thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738 for high lipids production. For this, ethyl methane sulfonate was used, which generated two effective thermo-tolerant mutants, M18 and M24 of Chlorella pyrenoidosa NCIM 2738, capable of surviving at temperature up to 47 °C and showing improved lipid and biomass yields. They showed 59.62% and 50.75% increase, respectively in lipid content compared to wild type at 30 °C, which could not grow at temperature above 35 °C. The novelty of this study lied in incorporation of PAM Flurometry with mutagenesis to generate thermo-tolerant mutants of C. pyrenoidosa and investigating the reasons for increased yields of mutants at cellular and photosynthetic levels with the aim to use them for commercial biodiesel production. [ABSTRACT FROM AUTHOR] |
| Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 118697852 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Sachdeva%2C+Neha%22">Sachdeva, Neha</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gupta%2C+Ravi+Prakash%22">Gupta, Ravi Prakash</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mathur%2C+Anshu+Shankar%22">Mathur, Anshu Shankar</searchLink><relatesTo>2</relatesTo><i> mathuras@indianoil.in</i><br /><searchLink fieldCode="AR" term="%22Tuli%2C+Deepak+Kumar%22">Tuli, Deepak Kumar</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Dec2016, Vol. 221, p576-587. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Lipids%22">Lipids</searchLink><br /><searchLink fieldCode="DE" term="%22Chlorella+pyrenoidosa%22">Chlorella pyrenoidosa</searchLink><br /><searchLink fieldCode="DE" term="%22Ethyl+methanesulfonate%22">Ethyl methanesulfonate</searchLink><br /><searchLink fieldCode="DE" term="%22Mutagenesis%22">Mutagenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Biodiesel+fuels%22">Biodiesel fuels</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The present study aimed to develop thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738 for high lipids production. For this, ethyl methane sulfonate was used, which generated two effective thermo-tolerant mutants, M18 and M24 of Chlorella pyrenoidosa NCIM 2738, capable of surviving at temperature up to 47 °C and showing improved lipid and biomass yields. They showed 59.62% and 50.75% increase, respectively in lipid content compared to wild type at 30 °C, which could not grow at temperature above 35 °C. The novelty of this study lied in incorporation of PAM Flurometry with mutagenesis to generate thermo-tolerant mutants of C. pyrenoidosa and investigating the reasons for increased yields of mutants at cellular and photosynthetic levels with the aim to use them for commercial biodiesel production. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.biortech.2016.09.049 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 576 Subjects: – SubjectFull: Lipids Type: general – SubjectFull: Chlorella pyrenoidosa Type: general – SubjectFull: Ethyl methanesulfonate Type: general – SubjectFull: Mutagenesis Type: general – SubjectFull: Biodiesel fuels Type: general Titles: – TitleFull: Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Sachdeva, Neha – PersonEntity: Name: NameFull: Gupta, Ravi Prakash – PersonEntity: Name: NameFull: Mathur, Anshu Shankar – PersonEntity: Name: NameFull: Tuli, Deepak Kumar IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 09608524 Numbering: – Type: volume Value: 221 Titles: – TitleFull: Bioresource Technology Type: main |
| ResultId | 1 |