Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738.
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| Title: | Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738. |
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| Authors: | Sachdeva, Neha1, Gupta, Ravi Prakash2, Mathur, Anshu Shankar2 mathuras@indianoil.in, Tuli, Deepak Kumar1 |
| Source: | Bioresource Technology. Dec2016, Vol. 221, p576-587. 12p. |
| Subjects: | Lipids, Chlorella pyrenoidosa, Ethyl methanesulfonate, Mutagenesis, Biodiesel fuels |
| Abstract: | The present study aimed to develop thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738 for high lipids production. For this, ethyl methane sulfonate was used, which generated two effective thermo-tolerant mutants, M18 and M24 of Chlorella pyrenoidosa NCIM 2738, capable of surviving at temperature up to 47 °C and showing improved lipid and biomass yields. They showed 59.62% and 50.75% increase, respectively in lipid content compared to wild type at 30 °C, which could not grow at temperature above 35 °C. The novelty of this study lied in incorporation of PAM Flurometry with mutagenesis to generate thermo-tolerant mutants of C. pyrenoidosa and investigating the reasons for increased yields of mutants at cellular and photosynthetic levels with the aim to use them for commercial biodiesel production. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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