View in EDS

Towards a transcriptome-based theranostic platform for unfavorable breast cancer phenotypes.

Saved in:
Bibliographic Details
Title: Towards a transcriptome-based theranostic platform for unfavorable breast cancer phenotypes.
Authors: Dobroff, Andrey S.1,2, D'Angelo, Sara1,2, Eckhardt, Bedrich L.3, Ferrara, Fortunato1,2, Staquicini, Daniela I.1,2, Cardó-Vila, Marina1,2, Staquicini, Fernanda I.1,2, Nunes, Diana N.4, Kisu Kim5, Driessen, Wouter H. P.6, Hajitou, Amin7, Lomo, Lesley C.1,8, Barry, Marc1,8, Krishnamurthy, Savitri9, Sahin, Aysegul9, Woodward, Wendy A.10, Prossnitz, Eric R.1,2, Anderson, Robin L.11, Dias-Neto, Emmanuel4,12, Brown-Glaberman, Ursa A.1,13
Source: Proceedings of the National Academy of Sciences of the United States of America. 11/8/2016, Vol. 113 Issue 45, p12780-12785. 6p.
Subjects: Inflammatory breast cancer, Breast cancer treatment, Oncology, Glucose-regulated proteins, Biomarkers, Bacteriophages
Abstract: Inflammatory breast carcinoma (IBC) is one of the most lethal forms of human breast cancer, and effective treatment for IBC is an unmet clinical need in contemporary oncology. Tumor-targeted theranostic approaches are emerging in precision medicine, but only a few specific biomarkers are available. Here we report up-regulation of the 78-kDa glucose-regulated protein (GRP78) in two independent discovery and validation sets of specimens derived from IBC patients, suggesting translational promise for clinical applications. We show that a GRP78-binding motif displayed on either bacteriophage or adeno-associated virus/phage (AAVP) particles or loop-grafted onto a human antibody fragment specifically targets orthotopic IBC and other aggressive breast cancer models in vivo. To evaluate the theranostic value, we used GRP78-targeting AAVP particles to deliver the human Herpes simplex virus thymidine kinase type-1 (HSVtk) transgene, obtaining simultaneous in vivo diagnosis through PET imaging and tumor treatment by selective activation of the prodrug ganciclovir at tumor sites. Translation of this AAVP system is expected simultaneously to image, monitor, and treat the IBC phenotype and possibly other aggressive (e.g., invasive and/or metastatic) subtypes of breast cancer, based on the inducible cell-surface expression of the stress-response chaperone GRP78, and possibly other cell-surface receptors in human tumors. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 119369104
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Towards a transcriptome-based theranostic platform for unfavorable breast cancer phenotypes.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Dobroff%2C+Andrey+S%2E%22">Dobroff, Andrey S.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22D'Angelo%2C+Sara%22">D'Angelo, Sara</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Eckhardt%2C+Bedrich+L%2E%22">Eckhardt, Bedrich L.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ferrara%2C+Fortunato%22">Ferrara, Fortunato</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Staquicini%2C+Daniela+I%2E%22">Staquicini, Daniela I.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Cardó-Vila%2C+Marina%22">Cardó-Vila, Marina</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Staquicini%2C+Fernanda+I%2E%22">Staquicini, Fernanda I.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Nunes%2C+Diana+N%2E%22">Nunes, Diana N.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Kisu+Kim%22">Kisu Kim</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Driessen%2C+Wouter+H%2E+P%2E%22">Driessen, Wouter H. P.</searchLink><relatesTo>6</relatesTo><br /><searchLink fieldCode="AR" term="%22Hajitou%2C+Amin%22">Hajitou, Amin</searchLink><relatesTo>7</relatesTo><br /><searchLink fieldCode="AR" term="%22Lomo%2C+Lesley+C%2E%22">Lomo, Lesley C.</searchLink><relatesTo>1,8</relatesTo><br /><searchLink fieldCode="AR" term="%22Barry%2C+Marc%22">Barry, Marc</searchLink><relatesTo>1,8</relatesTo><br /><searchLink fieldCode="AR" term="%22Krishnamurthy%2C+Savitri%22">Krishnamurthy, Savitri</searchLink><relatesTo>9</relatesTo><br /><searchLink fieldCode="AR" term="%22Sahin%2C+Aysegul%22">Sahin, Aysegul</searchLink><relatesTo>9</relatesTo><br /><searchLink fieldCode="AR" term="%22Woodward%2C+Wendy+A%2E%22">Woodward, Wendy A.</searchLink><relatesTo>10</relatesTo><br /><searchLink fieldCode="AR" term="%22Prossnitz%2C+Eric+R%2E%22">Prossnitz, Eric R.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Anderson%2C+Robin+L%2E%22">Anderson, Robin L.</searchLink><relatesTo>11</relatesTo><br /><searchLink fieldCode="AR" term="%22Dias-Neto%2C+Emmanuel%22">Dias-Neto, Emmanuel</searchLink><relatesTo>4,12</relatesTo><br /><searchLink fieldCode="AR" term="%22Brown-Glaberman%2C+Ursa+A%2E%22">Brown-Glaberman, Ursa A.</searchLink><relatesTo>1,13</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America%22">Proceedings of the National Academy of Sciences of the United States of America</searchLink>. 11/8/2016, Vol. 113 Issue 45, p12780-12785. 6p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Inflammatory+breast+cancer%22">Inflammatory breast cancer</searchLink><br /><searchLink fieldCode="DE" term="%22Breast+cancer+treatment%22">Breast cancer treatment</searchLink><br /><searchLink fieldCode="DE" term="%22Oncology%22">Oncology</searchLink><br /><searchLink fieldCode="DE" term="%22Glucose-regulated+proteins%22">Glucose-regulated proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteriophages%22">Bacteriophages</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Inflammatory breast carcinoma (IBC) is one of the most lethal forms of human breast cancer, and effective treatment for IBC is an unmet clinical need in contemporary oncology. Tumor-targeted theranostic approaches are emerging in precision medicine, but only a few specific biomarkers are available. Here we report up-regulation of the 78-kDa glucose-regulated protein (GRP78) in two independent discovery and validation sets of specimens derived from IBC patients, suggesting translational promise for clinical applications. We show that a GRP78-binding motif displayed on either bacteriophage or adeno-associated virus/phage (AAVP) particles or loop-grafted onto a human antibody fragment specifically targets orthotopic IBC and other aggressive breast cancer models in vivo. To evaluate the theranostic value, we used GRP78-targeting AAVP particles to deliver the human Herpes simplex virus thymidine kinase type-1 (HSVtk) transgene, obtaining simultaneous in vivo diagnosis through PET imaging and tumor treatment by selective activation of the prodrug ganciclovir at tumor sites. Translation of this AAVP system is expected simultaneously to image, monitor, and treat the IBC phenotype and possibly other aggressive (e.g., invasive and/or metastatic) subtypes of breast cancer, based on the inducible cell-surface expression of the stress-response chaperone GRP78, and possibly other cell-surface receptors in human tumors. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=119369104
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1073/pnas.1615288113
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 12780
    Subjects:
      – SubjectFull: Inflammatory breast cancer
        Type: general
      – SubjectFull: Breast cancer treatment
        Type: general
      – SubjectFull: Oncology
        Type: general
      – SubjectFull: Glucose-regulated proteins
        Type: general
      – SubjectFull: Biomarkers
        Type: general
      – SubjectFull: Bacteriophages
        Type: general
    Titles:
      – TitleFull: Towards a transcriptome-based theranostic platform for unfavorable breast cancer phenotypes.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Dobroff, Andrey S.
      – PersonEntity:
          Name:
            NameFull: D'Angelo, Sara
      – PersonEntity:
          Name:
            NameFull: Eckhardt, Bedrich L.
      – PersonEntity:
          Name:
            NameFull: Ferrara, Fortunato
      – PersonEntity:
          Name:
            NameFull: Staquicini, Daniela I.
      – PersonEntity:
          Name:
            NameFull: Cardó-Vila, Marina
      – PersonEntity:
          Name:
            NameFull: Staquicini, Fernanda I.
      – PersonEntity:
          Name:
            NameFull: Nunes, Diana N.
      – PersonEntity:
          Name:
            NameFull: Kisu Kim
      – PersonEntity:
          Name:
            NameFull: Driessen, Wouter H. P.
      – PersonEntity:
          Name:
            NameFull: Hajitou, Amin
      – PersonEntity:
          Name:
            NameFull: Lomo, Lesley C.
      – PersonEntity:
          Name:
            NameFull: Barry, Marc
      – PersonEntity:
          Name:
            NameFull: Krishnamurthy, Savitri
      – PersonEntity:
          Name:
            NameFull: Sahin, Aysegul
      – PersonEntity:
          Name:
            NameFull: Woodward, Wendy A.
      – PersonEntity:
          Name:
            NameFull: Prossnitz, Eric R.
      – PersonEntity:
          Name:
            NameFull: Anderson, Robin L.
      – PersonEntity:
          Name:
            NameFull: Dias-Neto, Emmanuel
      – PersonEntity:
          Name:
            NameFull: Brown-Glaberman, Ursa A.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 08
              M: 11
              Text: 11/8/2016
              Type: published
              Y: 2016
          Identifiers:
            – Type: issn-print
              Value: 00278424
          Numbering:
            – Type: volume
              Value: 113
            – Type: issue
              Value: 45
          Titles:
            – TitleFull: Proceedings of the National Academy of Sciences of the United States of America
              Type: main
ResultId 1