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Dietary Tryptophan Enhanced the Expression of Tight Junction Protein ZO-1 in Intestine.

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Title: Dietary Tryptophan Enhanced the Expression of Tight Junction Protein ZO-1 in Intestine.
Authors: Liu, Wenhui1, Mi, Shumei1, Ruan, Zheng1 ruanzheng@ncu.edu.cn, Li, Jing1, Shu, Xugang1, Yao, Kang2, Jiang, Min1, Deng, Zeyuan1
Source: Journal of Food Science (John Wiley & Sons, Inc.). Feb2017, Vol. 82 Issue 2, p562-567. 6p.
Subjects: Tryptophan, Intestinal development, Physiological effects of amino acids, Tight junctions, Occludins, Swine physiology
Abstract (English): Dietary amino acids provide various beneficial effects for our health. The aim of the present study was to assess the effects of tryptophan (Trp) supplementation on barrier function. Ninety-six healthy finishing pigs (initial body weight 51.49 ± 1.12 kg) were randomly allocated into 2 treatment groups, control group, and 0.2% Trp group. The control group was fed the basal diet, and 0.2% Trp group was fed basal diet plus 0.2% Trp. The trial period is 60 d. Compared with control group, the mRNA abundance of claudin-3 and zonula occluden-1 (ZO-1) in the jejunum in 0.2% Trp group ( P < 0.05) was increased. According to immunohistochemistry and immunoblotting test, the expression of ZO-1 in jejunum in 0.2% Trp group was also significantly increased compared with the control group ( P < 0.05). These results revealed that Trp enhanced the expression of tight junction protein ZO-1 in the intestine of pig model. Trp may be potential and beneficial dietary functional factor for regulating the intestinal development and inhibiting intestinal aging. [ABSTRACT FROM AUTHOR]
Abstract (Portuguese): Practical Application The work has beneficial for intestinal health, such as promoting the gut development and inhibiting intestinal aging for the aged. The research has direct commercial application for amino acids supplementation or prebiotics. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Dietary amino acids provide various beneficial effects for our health. The aim of the present study was to assess the effects of tryptophan (Trp) supplementation on barrier function. Ninety-six healthy finishing pigs (initial body weight 51.49 ± 1.12 kg) were randomly allocated into 2 treatment groups, control group, and 0.2% Trp group. The control group was fed the basal diet, and 0.2% Trp group was fed basal diet plus 0.2% Trp. The trial period is 60 d. Compared with control group, the mRNA abundance of claudin-3 and zonula occluden-1 (ZO-1) in the jejunum in 0.2% Trp group ( P < 0.05) was increased. According to immunohistochemistry and immunoblotting test, the expression of ZO-1 in jejunum in 0.2% Trp group was also significantly increased compared with the control group ( P < 0.05). These results revealed that Trp enhanced the expression of tight junction protein ZO-1 in the intestine of pig model. Trp may be potential and beneficial dietary functional factor for regulating the intestinal development and inhibiting intestinal aging. [ABSTRACT FROM AUTHOR]
ISSN:00221147
DOI:10.1111/1750-3841.13603