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Proteome response of fish under multiple stress exposure: Effects of pesticide mixtures and temperature increase.

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Title: Proteome response of fish under multiple stress exposure: Effects of pesticide mixtures and temperature increase.
Authors: Gandar, Allison1, Laffaille, Pascal1, Marty-Gasset, Nathalie2, Viala, Didier3,4, Molette, Caroline2, Jean, Séverine1 severine.jean@ensat.fr
Source: Aquatic Toxicology. Mar2017, Vol. 184, p61-77. 17p.
Subjects: Fish physiology, Physiological effects of pesticides, Physiological effects of temperature, Proteomics, Physiological stress
Abstract: Aquatic systems can be subjected to multiple stressors, including pollutant cocktails and elevated temperature. Evaluating the combined effects of these stressors on organisms is a great challenge in environmental sciences. To the best of our knowledge, this is the first study to assess the molecular stress response of an aquatic fish species subjected to individual and combined pesticide mixtures and increased temperatures. For that, goldfish ( Carassius auratus ) were acclimated to two different temperatures (22 and 32 °C) for 15 days. They were then exposed for 96 h to a cocktail of herbicides and fungicides (S-metolachlor, isoproturon, linuron, atrazine-desethyl, aclonifen, pendimethalin and tebuconazole) at two environmentally relevant concentrations (total concentrations of 8.4 μg L −1 and 42 μg L −1 ) at these two temperatures (22 and 32 °C). The molecular response in liver was assessed by 2D-proteomics. Identified proteins were integrated using pathway enrichment analysis software to determine the biological functions involved in the individual or combined stress responses and to predict the potential deleterious outcomes. The pesticide mixtures elicited pathways involved in cellular stress response, carbohydrate, protein and lipid metabolisms, methionine cycle, cellular functions, cell structure and death control, with concentration- and temperature-dependent profiles of response. We found that combined temperature increase and pesticide exposure affected the cellular stress response: the effects of oxidative stress were more marked and there was a deregulation of the cell cycle via apoptosis inhibition. Moreover a decrease in the formation of glucose by liver and in ketogenic activity was observed in this multi-stress condition. The decrease in both pathways could reflect a shift from a metabolic compensation strategy to a conservation state. Taken together, our results showed (1) that environmental cocktails of herbicides and fungicides induced important changes in pathways involved in metabolism, cell structure and cell cycle, with possible deleterious outcomes at higher biological scales and (2) that increasing temperature could affect the response of fish to pesticide exposure. [ABSTRACT FROM AUTHOR]
Copyright of Aquatic Toxicology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 121220844
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Proteome response of fish under multiple stress exposure: Effects of pesticide mixtures and temperature increase.
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  Data: <searchLink fieldCode="JN" term="%22Aquatic+Toxicology%22">Aquatic Toxicology</searchLink>. Mar2017, Vol. 184, p61-77. 17p.
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  Data: Aquatic systems can be subjected to multiple stressors, including pollutant cocktails and elevated temperature. Evaluating the combined effects of these stressors on organisms is a great challenge in environmental sciences. To the best of our knowledge, this is the first study to assess the molecular stress response of an aquatic fish species subjected to individual and combined pesticide mixtures and increased temperatures. For that, goldfish ( Carassius auratus ) were acclimated to two different temperatures (22 and 32 °C) for 15 days. They were then exposed for 96 h to a cocktail of herbicides and fungicides (S-metolachlor, isoproturon, linuron, atrazine-desethyl, aclonifen, pendimethalin and tebuconazole) at two environmentally relevant concentrations (total concentrations of 8.4 μg L −1 and 42 μg L −1 ) at these two temperatures (22 and 32 °C). The molecular response in liver was assessed by 2D-proteomics. Identified proteins were integrated using pathway enrichment analysis software to determine the biological functions involved in the individual or combined stress responses and to predict the potential deleterious outcomes. The pesticide mixtures elicited pathways involved in cellular stress response, carbohydrate, protein and lipid metabolisms, methionine cycle, cellular functions, cell structure and death control, with concentration- and temperature-dependent profiles of response. We found that combined temperature increase and pesticide exposure affected the cellular stress response: the effects of oxidative stress were more marked and there was a deregulation of the cell cycle via apoptosis inhibition. Moreover a decrease in the formation of glucose by liver and in ketogenic activity was observed in this multi-stress condition. The decrease in both pathways could reflect a shift from a metabolic compensation strategy to a conservation state. Taken together, our results showed (1) that environmental cocktails of herbicides and fungicides induced important changes in pathways involved in metabolism, cell structure and cell cycle, with possible deleterious outcomes at higher biological scales and (2) that increasing temperature could affect the response of fish to pesticide exposure. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Aquatic Toxicology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.aquatox.2017.01.004
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      – Code: eng
        Text: English
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        PageCount: 17
        StartPage: 61
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      – SubjectFull: Fish physiology
        Type: general
      – SubjectFull: Physiological effects of pesticides
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      – SubjectFull: Physiological effects of temperature
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      – SubjectFull: Proteomics
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      – SubjectFull: Physiological stress
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      – TitleFull: Proteome response of fish under multiple stress exposure: Effects of pesticide mixtures and temperature increase.
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            NameFull: Gandar, Allison
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              Text: Mar2017
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              Y: 2017
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              Value: 184
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