The mechanism of improved intracellular organic selenium and glutathione contents in selenium-enriched Candida utilis by acid stress.
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| Title: | The mechanism of improved intracellular organic selenium and glutathione contents in selenium-enriched Candida utilis by acid stress. |
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| Authors: | Zhang, Gao-Chuan1, Wang, Da-Hui1, Wang, Dong-Hua1, Wei, Gong-Yuan1 weigy@suda.edu.cn |
| Source: | Applied Microbiology & Biotechnology. Mar2017, Vol. 101 Issue 5, p2131-2141. 11p. |
| Subjects: | Candida utilis, Effect of acids on fungi, Selenium in the body, Yeast physiology, Glutathione, Adenosine triphosphatase, RNA sequencing |
| Abstract: | Batch culture of Candida utilis CCTCC M 209298 for the preparation of selenium (Se)-enriched yeast was carried out under different pH conditions, and maximal intracellular organic Se and glutathione (GSH) contents were obtained in a moderate acid stress environment (pH 3.5). In order to elucidate the physiological mechanism of improved performance of Se-enriched yeast by acid stress, assays of the key enzymes involved in GSH biosynthesis and determinations of energy supply and regeneration were performed. The results indicated that moderate acid stress increased the activity of γ-glutamylcysteine synthetase and the ratios of NADH/NAD and ATP/ADP, although no significant changes in intracellular pH were observed. In addition, the molecular mechanism of moderate acid stress favoring the improvement of Se-yeast performance was revealed by comparing whole transcriptomes of yeast cells cultured at pH 3.5 and 5.5. Comparative analysis of RNA-Seq data indicated that 882 genes were significantly up-regulated by moderate acid stress. Functional annotation of the up-regulated genes based on gene ontology and the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway showed that these genes are involved in ATP synthesis and sulfur metabolism, including the biosynthesis of methionine, cysteine, and GSH in yeast cells. Increased intracellular ATP supply and more amounts of sulfur-containing substances in turn contributed to NaSeO assimilation and biotransformation, which ultimately improved the performance of the Se-enriched C. utilis. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: The mechanism of improved intracellular organic selenium and glutathione contents in selenium-enriched Candida utilis by acid stress. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zhang%2C+Gao-Chuan%22">Zhang, Gao-Chuan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Da-Hui%22">Wang, Da-Hui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Dong-Hua%22">Wang, Dong-Hua</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wei%2C+Gong-Yuan%22">Wei, Gong-Yuan</searchLink><relatesTo>1</relatesTo><i> weigy@suda.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Mar2017, Vol. 101 Issue 5, p2131-2141. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Candida+utilis%22">Candida utilis</searchLink><br /><searchLink fieldCode="DE" term="%22Effect+of+acids+on+fungi%22">Effect of acids on fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Selenium+in+the+body%22">Selenium in the body</searchLink><br /><searchLink fieldCode="DE" term="%22Yeast+physiology%22">Yeast physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Glutathione%22">Glutathione</searchLink><br /><searchLink fieldCode="DE" term="%22Adenosine+triphosphatase%22">Adenosine triphosphatase</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Batch culture of Candida utilis CCTCC M 209298 for the preparation of selenium (Se)-enriched yeast was carried out under different pH conditions, and maximal intracellular organic Se and glutathione (GSH) contents were obtained in a moderate acid stress environment (pH 3.5). In order to elucidate the physiological mechanism of improved performance of Se-enriched yeast by acid stress, assays of the key enzymes involved in GSH biosynthesis and determinations of energy supply and regeneration were performed. The results indicated that moderate acid stress increased the activity of γ-glutamylcysteine synthetase and the ratios of NADH/NAD and ATP/ADP, although no significant changes in intracellular pH were observed. In addition, the molecular mechanism of moderate acid stress favoring the improvement of Se-yeast performance was revealed by comparing whole transcriptomes of yeast cells cultured at pH 3.5 and 5.5. Comparative analysis of RNA-Seq data indicated that 882 genes were significantly up-regulated by moderate acid stress. Functional annotation of the up-regulated genes based on gene ontology and the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway showed that these genes are involved in ATP synthesis and sulfur metabolism, including the biosynthesis of methionine, cysteine, and GSH in yeast cells. Increased intracellular ATP supply and more amounts of sulfur-containing substances in turn contributed to NaSeO assimilation and biotransformation, which ultimately improved the performance of the Se-enriched C. utilis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-016-8016-4 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 2131 Subjects: – SubjectFull: Candida utilis Type: general – SubjectFull: Effect of acids on fungi Type: general – SubjectFull: Selenium in the body Type: general – SubjectFull: Yeast physiology Type: general – SubjectFull: Glutathione Type: general – SubjectFull: Adenosine triphosphatase Type: general – SubjectFull: RNA sequencing Type: general Titles: – TitleFull: The mechanism of improved intracellular organic selenium and glutathione contents in selenium-enriched Candida utilis by acid stress. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zhang, Gao-Chuan – PersonEntity: Name: NameFull: Wang, Da-Hui – PersonEntity: Name: NameFull: Wang, Dong-Hua – PersonEntity: Name: NameFull: Wei, Gong-Yuan IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 101 – Type: issue Value: 5 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
