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Advantages of time-resolved fluorescent nanobeads compared with fluorescent submicrospheres, quantum dots, and colloidal gold as label in lateral flow assays for detection of ractopamine.

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Title: Advantages of time-resolved fluorescent nanobeads compared with fluorescent submicrospheres, quantum dots, and colloidal gold as label in lateral flow assays for detection of ractopamine.
Authors: Hu, Li-Ming1, Luo, Kai1, Xia, Jun2, Xu, Guo-Mao2, Wu, Cheng-Hui1, Han, Jiao-Jiao1, Zhang, Gang-Gang1, Liu, Miao1, Lai, Wei-Hua1 talktolaiwh@163.com
Source: Biosensors & Bioelectronics. May2017, Vol. 91, p95-103. 9p.
Subjects: Quantum dots, Colloidal gold, Ractopamine, Fluorescence, Urinalysis
Abstract: Label selection is a critical factor for improving the sensitivity of lateral flow assay. Time-resolved fluorescent nanobeads, fluorescent submicrospheres, quantum dots, and colloidal gold-based lateral flow assay (TRFN-LFA, FM-LFA, QD-LFA, and CG-LFA) were first systematically compared for the quantitative detection of ractopamine in swine urine based on competitive format. The limits of detection (LOD) of TRFN-LFA, FM-LFA, QD-LFA, and CG-LFA were 7.2, 14.7, 23.6, and 40.1 pg/mL in swine urine samples, respectively. The sensitivity of TRFN-LFA was highest. In the quantitative determination of ractopamine (RAC) in swine urine samples, TRFN-LFA exhibited a wide linear range of 5 pg/mL to 2500 pg/mL with a reliable coefficient of correlation ( R 2 =0.9803). Relatively narrow linear ranges of 10–500 pg/mL (FM-LFA) and 25–2500 pg/mL (QD-LFA and CG-LFA) were acquired. Approximately 0.005 µg of anti-RAC poly antibody (pAb) was used in each TRFN-LFA test strip, whereas 0.02, 0.054, and 0.15 µg of pAb were used in each of the FM-LFA, QD-LFA, and CG-LFA test strips, respectively. In addition, TRFN-LFA required the least RAC-BSA antigens and exhibited the shortest detection time compared with the other lateral flow assays. Analysis of the RAC in swine urine samples showed that the result of TRFN-LFA was consistent with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and a commercial enzyme-linked immunosorbent assay (ELISA) kit. [ABSTRACT FROM AUTHOR]
Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Advantages of time-resolved fluorescent nanobeads compared with fluorescent submicrospheres, quantum dots, and colloidal gold as label in lateral flow assays for detection of ractopamine.
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  Data: <searchLink fieldCode="AR" term="%22Hu%2C+Li-Ming%22">Hu, Li-Ming</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Luo%2C+Kai%22">Luo, Kai</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xia%2C+Jun%22">Xia, Jun</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xu%2C+Guo-Mao%22">Xu, Guo-Mao</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Cheng-Hui%22">Wu, Cheng-Hui</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Han%2C+Jiao-Jiao%22">Han, Jiao-Jiao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Gang-Gang%22">Zhang, Gang-Gang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Miao%22">Liu, Miao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lai%2C+Wei-Hua%22">Lai, Wei-Hua</searchLink><relatesTo>1</relatesTo><i> talktolaiwh@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Biosensors+%26+Bioelectronics%22">Biosensors & Bioelectronics</searchLink>. May2017, Vol. 91, p95-103. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Quantum+dots%22">Quantum dots</searchLink><br /><searchLink fieldCode="DE" term="%22Colloidal+gold%22">Colloidal gold</searchLink><br /><searchLink fieldCode="DE" term="%22Ractopamine%22">Ractopamine</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence%22">Fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Urinalysis%22">Urinalysis</searchLink>
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  Label: Abstract
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  Data: Label selection is a critical factor for improving the sensitivity of lateral flow assay. Time-resolved fluorescent nanobeads, fluorescent submicrospheres, quantum dots, and colloidal gold-based lateral flow assay (TRFN-LFA, FM-LFA, QD-LFA, and CG-LFA) were first systematically compared for the quantitative detection of ractopamine in swine urine based on competitive format. The limits of detection (LOD) of TRFN-LFA, FM-LFA, QD-LFA, and CG-LFA were 7.2, 14.7, 23.6, and 40.1 pg/mL in swine urine samples, respectively. The sensitivity of TRFN-LFA was highest. In the quantitative determination of ractopamine (RAC) in swine urine samples, TRFN-LFA exhibited a wide linear range of 5 pg/mL to 2500 pg/mL with a reliable coefficient of correlation ( R 2 =0.9803). Relatively narrow linear ranges of 10–500 pg/mL (FM-LFA) and 25–2500 pg/mL (QD-LFA and CG-LFA) were acquired. Approximately 0.005 µg of anti-RAC poly antibody (pAb) was used in each TRFN-LFA test strip, whereas 0.02, 0.054, and 0.15 µg of pAb were used in each of the FM-LFA, QD-LFA, and CG-LFA test strips, respectively. In addition, TRFN-LFA required the least RAC-BSA antigens and exhibited the shortest detection time compared with the other lateral flow assays. Analysis of the RAC in swine urine samples showed that the result of TRFN-LFA was consistent with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and a commercial enzyme-linked immunosorbent assay (ELISA) kit. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – SubjectFull: Ractopamine
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              Text: May2017
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