Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding.
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| Title: | Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding. |
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| Authors: | Ezhuthupurakkal, Preedia Babu1, Polaki, Lokeswara Rao1, Suyavaran, Arumugam1, Subastri, Ariraman1, Sujatha, Venugopal2, Thirunavukkarasu, Chinnasamy1 tarasu.bbm@pondiuni.edu.in |
| Source: | Materials Science & Engineering: C. May2017, Vol. 74, p597-608. 12p. |
| Subjects: | Selenium, Allium, Lymphoid tissue, Deoxyribose, DNA |
| Abstract: | Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for synthesis of SeNPs. The present study reports an aqueous extract of Allium sativum ( AqEAS ) plug-up the current need. Modern spectroscopic, microscopic and gravimetric techniques were employed to characterize the synthesized nanoparticles. Characterization studies revealed the formation of crystalline spherical shaped SeNPs. FTIR spectrum brings out the presence of different functional groups in AqEAS , which influence the SeNPs formation and stabilization. Furthermore the different aspects of the interaction between SeNPs and CT-DNA were scrutinized by various spectroscopic and cyclic voltametric studies. The results reveals the intercalation and groove binding mode of interaction of SeNPs with stacked base pair of CT-DNA. The Stern–Volmer quenching constant ( K SV ) were found to be 7.02 × 10 6 Mˉ 1 (ethidium bromide), 4.22 × 10 6 Mˉ 1 (acridine orange) and 7.6 × 10 6 Mˉ 1 (Hoechst) indicating strong binding of SeNPs with CT–DNA. The SeNPs - CT-DNA interactions were directly visualized by atomic force microscopy. The present study unveils the cost effective, innocuous, highly stable SeNPs intricate mechanism of DNA interaction, which will be a milestone in DNA targeted chemotherapy. [ABSTRACT FROM AUTHOR] |
| Copyright of Materials Science & Engineering: C is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 121492463 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ezhuthupurakkal%2C+Preedia+Babu%22">Ezhuthupurakkal, Preedia Babu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Polaki%2C+Lokeswara+Rao%22">Polaki, Lokeswara Rao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Suyavaran%2C+Arumugam%22">Suyavaran, Arumugam</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Subastri%2C+Ariraman%22">Subastri, Ariraman</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sujatha%2C+Venugopal%22">Sujatha, Venugopal</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Thirunavukkarasu%2C+Chinnasamy%22">Thirunavukkarasu, Chinnasamy</searchLink><relatesTo>1</relatesTo><i> tarasu.bbm@pondiuni.edu.in</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Materials+Science+%26+Engineering%3A+C%22">Materials Science & Engineering: C</searchLink>. May2017, Vol. 74, p597-608. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Selenium%22">Selenium</searchLink><br /><searchLink fieldCode="DE" term="%22Allium%22">Allium</searchLink><br /><searchLink fieldCode="DE" term="%22Lymphoid+tissue%22">Lymphoid tissue</searchLink><br /><searchLink fieldCode="DE" term="%22Deoxyribose%22">Deoxyribose</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for synthesis of SeNPs. The present study reports an aqueous extract of Allium sativum ( AqEAS ) plug-up the current need. Modern spectroscopic, microscopic and gravimetric techniques were employed to characterize the synthesized nanoparticles. Characterization studies revealed the formation of crystalline spherical shaped SeNPs. FTIR spectrum brings out the presence of different functional groups in AqEAS , which influence the SeNPs formation and stabilization. Furthermore the different aspects of the interaction between SeNPs and CT-DNA were scrutinized by various spectroscopic and cyclic voltametric studies. The results reveals the intercalation and groove binding mode of interaction of SeNPs with stacked base pair of CT-DNA. The Stern–Volmer quenching constant ( K SV ) were found to be 7.02 × 10 6 Mˉ 1 (ethidium bromide), 4.22 × 10 6 Mˉ 1 (acridine orange) and 7.6 × 10 6 Mˉ 1 (Hoechst) indicating strong binding of SeNPs with CT–DNA. The SeNPs - CT-DNA interactions were directly visualized by atomic force microscopy. The present study unveils the cost effective, innocuous, highly stable SeNPs intricate mechanism of DNA interaction, which will be a milestone in DNA targeted chemotherapy. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Materials Science & Engineering: C is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.msec.2017.02.003 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 597 Subjects: – SubjectFull: Selenium Type: general – SubjectFull: Allium Type: general – SubjectFull: Lymphoid tissue Type: general – SubjectFull: Deoxyribose Type: general – SubjectFull: DNA Type: general Titles: – TitleFull: Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ezhuthupurakkal, Preedia Babu – PersonEntity: Name: NameFull: Polaki, Lokeswara Rao – PersonEntity: Name: NameFull: Suyavaran, Arumugam – PersonEntity: Name: NameFull: Subastri, Ariraman – PersonEntity: Name: NameFull: Sujatha, Venugopal – PersonEntity: Name: NameFull: Thirunavukkarasu, Chinnasamy IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 09284931 Numbering: – Type: volume Value: 74 Titles: – TitleFull: Materials Science & Engineering: C Type: main |
| ResultId | 1 |
