Development of a NanoBioAnalytical platform for "on-chip" qualification and quantification of platelet-derived microparticles.
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| Title: | Development of a NanoBioAnalytical platform for "on-chip" qualification and quantification of platelet-derived microparticles. |
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| Authors: | Obeid, Sameh1, Ceroi, Adam2, Mourey, Guillaume2, Saas, Philippe2, Elie-Caille, Celine1, Boireau, Wilfrid1 wboireau@femto-st.fr |
| Source: | Biosensors & Bioelectronics. Jul2017, Vol. 93, p250-259. 10p. |
| Subjects: | Blood platelets, Surface plasmon resonance, Atomic force microscopy, Virus-like particles, Detection limit |
| Abstract: | Blood microparticles (MPs) are small membrane vesicles (50–1000 nm), derived from different cell types. They are known to play important roles in various biological processes and also recognized as potential biomarkers of various health disorders. Different methods are currently used for the detection and characterization of MPs, but none of these methods is capable to quantify and qualify total MPs at the same time, hence, there is a need to develop a new approach for simultaneous detection, characterization and quantification of microparticles. Here we show the potential of surface plasmon resonance (SPR) method coupled to atomic force microscopy (AFM) to quantify and qualify platelet-derived microparticles (PMPs), on the whole nano-to micro-meter scale. The different subpopulations of microparticles could be determined via their capture onto the surface using specific ligands. In order to verify the correlation between the capture level and the microparticles concentration in solution, two calibration standards were used: Virus-Like Particles (VLPs) and synthetic beads with a mean diameter of 53 nm and 920 nm respectively. The AFM analysis of the biochip surface allowed metrological analysis of captured PMPs and revealed that more than 95% of PMPs were smaller than 300 nm. Our results suggest that our NanoBioAnalytical platform, combining SPR and AFM, is a suitable method for a sensitive, reproducible, label-free characterization and quantification of MPs over a wide concentration range (≈10 7 to 10 12 particles/mL; with a limit of detection (LOD) in the lowest ng/µL range) which matches with their typical concentrations in blood. [ABSTRACT FROM AUTHOR] |
| Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 122038751 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development of a NanoBioAnalytical platform for "on-chip" qualification and quantification of platelet-derived microparticles. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Obeid%2C+Sameh%22">Obeid, Sameh</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ceroi%2C+Adam%22">Ceroi, Adam</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mourey%2C+Guillaume%22">Mourey, Guillaume</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Saas%2C+Philippe%22">Saas, Philippe</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Elie-Caille%2C+Celine%22">Elie-Caille, Celine</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Boireau%2C+Wilfrid%22">Boireau, Wilfrid</searchLink><relatesTo>1</relatesTo><i> wboireau@femto-st.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biosensors+%26+Bioelectronics%22">Biosensors & Bioelectronics</searchLink>. Jul2017, Vol. 93, p250-259. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Blood+platelets%22">Blood platelets</searchLink><br /><searchLink fieldCode="DE" term="%22Surface+plasmon+resonance%22">Surface plasmon resonance</searchLink><br /><searchLink fieldCode="DE" term="%22Atomic+force+microscopy%22">Atomic force microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Virus-like+particles%22">Virus-like particles</searchLink><br /><searchLink fieldCode="DE" term="%22Detection+limit%22">Detection limit</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Blood microparticles (MPs) are small membrane vesicles (50–1000 nm), derived from different cell types. They are known to play important roles in various biological processes and also recognized as potential biomarkers of various health disorders. Different methods are currently used for the detection and characterization of MPs, but none of these methods is capable to quantify and qualify total MPs at the same time, hence, there is a need to develop a new approach for simultaneous detection, characterization and quantification of microparticles. Here we show the potential of surface plasmon resonance (SPR) method coupled to atomic force microscopy (AFM) to quantify and qualify platelet-derived microparticles (PMPs), on the whole nano-to micro-meter scale. The different subpopulations of microparticles could be determined via their capture onto the surface using specific ligands. In order to verify the correlation between the capture level and the microparticles concentration in solution, two calibration standards were used: Virus-Like Particles (VLPs) and synthetic beads with a mean diameter of 53 nm and 920 nm respectively. The AFM analysis of the biochip surface allowed metrological analysis of captured PMPs and revealed that more than 95% of PMPs were smaller than 300 nm. Our results suggest that our NanoBioAnalytical platform, combining SPR and AFM, is a suitable method for a sensitive, reproducible, label-free characterization and quantification of MPs over a wide concentration range (≈10 7 to 10 12 particles/mL; with a limit of detection (LOD) in the lowest ng/µL range) which matches with their typical concentrations in blood. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.bios.2016.08.100 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 250 Subjects: – SubjectFull: Blood platelets Type: general – SubjectFull: Surface plasmon resonance Type: general – SubjectFull: Atomic force microscopy Type: general – SubjectFull: Virus-like particles Type: general – SubjectFull: Detection limit Type: general Titles: – TitleFull: Development of a NanoBioAnalytical platform for "on-chip" qualification and quantification of platelet-derived microparticles. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Obeid, Sameh – PersonEntity: Name: NameFull: Ceroi, Adam – PersonEntity: Name: NameFull: Mourey, Guillaume – PersonEntity: Name: NameFull: Saas, Philippe – PersonEntity: Name: NameFull: Elie-Caille, Celine – PersonEntity: Name: NameFull: Boireau, Wilfrid IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 07 Text: Jul2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 09565663 Numbering: – Type: volume Value: 93 Titles: – TitleFull: Biosensors & Bioelectronics Type: main |
| ResultId | 1 |
