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Use of magnetically disentangled thiolated carbon nanotubes as a label-free impedimetric genosensor for detecting canine Leishmania spp. infection.

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Title: Use of magnetically disentangled thiolated carbon nanotubes as a label-free impedimetric genosensor for detecting canine Leishmania spp. infection.
Authors: Frias, Isaac A. M.1, Andrade, Cesar A. S.2, Balbino, Valdir Q.3, de Melo, Celso P.4 celso@df.ufpe.br
Source: Carbon. Jun2017, Vol. 117, p33-40. 8p.
Subjects: Carbon nanotubes, Carbon nanofibers, DNA analysis, Nanotubes, Carbon nanohorns
Abstract: We discuss the development of a thiolated carbon nanotube (ThNT) transducer for femtomolar detection of Leishmania spp. (CL) DNA based on the use of electrical impedance spectroscopy. Aminated short single strands probes of the chosen DNA target were covalently linked to carboxyl-functionalized ThNTs, anchored upon ultrathin Au films. The genosensors exhibit a less resistive behavior when an external magnetic field was applied to forcefully disentangle the ThNTs during their covalent immobilization, than when immobilized in their natural randomly oriented form. Under disentangled conditions the sensors presented a linear detection of parasite DNA in the 0.1 to 98.3 fg/μL range. The lower limit corresponds to a CL DNA concentration of 15 fM in canine genomic samples extracted directly from the blood of infected animals. The covalent immobilization of the ThNTs at the electrode surface reduces their tendency to peeling off. As a result, one could recycle the sensors after each experiment by rinsing them with hot water and use them for at least 5 successive times, with a relative standard deviation lower than 5%. Since no amplification of the target DNA is required, we suggest that use of these genosensors can represent a simple and yet accurate approach for the fast-molecular diagnosis of canine leishmaniasis. [ABSTRACT FROM AUTHOR]
Copyright of Carbon is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 122316829
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  Data: We discuss the development of a thiolated carbon nanotube (ThNT) transducer for femtomolar detection of Leishmania spp. (CL) DNA based on the use of electrical impedance spectroscopy. Aminated short single strands probes of the chosen DNA target were covalently linked to carboxyl-functionalized ThNTs, anchored upon ultrathin Au films. The genosensors exhibit a less resistive behavior when an external magnetic field was applied to forcefully disentangle the ThNTs during their covalent immobilization, than when immobilized in their natural randomly oriented form. Under disentangled conditions the sensors presented a linear detection of parasite DNA in the 0.1 to 98.3 fg/μL range. The lower limit corresponds to a CL DNA concentration of 15 fM in canine genomic samples extracted directly from the blood of infected animals. The covalent immobilization of the ThNTs at the electrode surface reduces their tendency to peeling off. As a result, one could recycle the sensors after each experiment by rinsing them with hot water and use them for at least 5 successive times, with a relative standard deviation lower than 5%. Since no amplification of the target DNA is required, we suggest that use of these genosensors can represent a simple and yet accurate approach for the fast-molecular diagnosis of canine leishmaniasis. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Carbon is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.carbon.2017.02.031
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        Text: English
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      – SubjectFull: DNA analysis
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      – TitleFull: Use of magnetically disentangled thiolated carbon nanotubes as a label-free impedimetric genosensor for detecting canine Leishmania spp. infection.
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              Text: Jun2017
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