View in EDS

Paper-based analytical devices for direct electrochemical detection of free IAA and SA in plant samples with the weight of several milligrams.

Saved in:
Bibliographic Details
Title: Paper-based analytical devices for direct electrochemical detection of free IAA and SA in plant samples with the weight of several milligrams.
Authors: Sun, Li-Jun1,2, Xie, Yuan1, Yan, Yong-Feng1, Yang, Haibing3, Gu, Hai-Ying1 hygu@ntu.edu.cn, Bao, Ning1 ningbao@ntu.edu.cn
Source: Sensors & Actuators B: Chemical. Aug2017, Vol. 247, p336-342. 7p.
Subjects: Electrochemical sensors, Acetic acid, Salicylic acid, Auxin, Plant hormones
Abstract: It is not easy to directly obtain concentrations of auxin (mainly indole-3-acetic acid, or IAA) and other phytohormones in different locations of individual plants although such information is critical for the study of their functions. Here we developed a paper-based analytical device consisting of a disposable working electrode to provide a platform for direct and simultaneous detection of free IAA and salicylic acid (SA) in different parts of pea seedlings with the weights of several milligrams. The double sided conductive carbon tape modified with carbon nanotube was used as the disposable working electrodes after they were treated with oxygen plasma. Different parts of pea seedlings were applied on the surface of working electrodes for direct electrochemical detection of free IAA and SA in paper-based analytical devices. The requirement of solution volumes with only 10 microliters made it possible to quantify free IAA and SA at the level of ng. Our results suggested that large amounts of inherent IAA and SA could be lost because of complicated and time-consuming preparation of samples in traditional gas/liquid chromatography-mass spectrometry methods Our approach not only avoids such steps but also provides concentrations of IAA and SA in different zones of individual pea seedlings. The obtained results could be applied for the study of individual differences and interactions between IAA and SA in development of peas. This strategy not only paved the foundation for further investigation of regulating mechanisms of free IAA and its interaction with other phytohormones but also might provide an alternative approach for detection of free electroactive biomolecules in other living organs. [ABSTRACT FROM AUTHOR]
Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 122699289
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Paper-based analytical devices for direct electrochemical detection of free IAA and SA in plant samples with the weight of several milligrams.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Sun%2C+Li-Jun%22">Sun, Li-Jun</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xie%2C+Yuan%22">Xie, Yuan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yan%2C+Yong-Feng%22">Yan, Yong-Feng</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang%2C+Haibing%22">Yang, Haibing</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Gu%2C+Hai-Ying%22">Gu, Hai-Ying</searchLink><relatesTo>1</relatesTo><i> hygu@ntu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Bao%2C+Ning%22">Bao, Ning</searchLink><relatesTo>1</relatesTo><i> ningbao@ntu.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Sensors+%26+Actuators+B%3A+Chemical%22">Sensors & Actuators B: Chemical</searchLink>. Aug2017, Vol. 247, p336-342. 7p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Electrochemical+sensors%22">Electrochemical sensors</searchLink><br /><searchLink fieldCode="DE" term="%22Acetic+acid%22">Acetic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Salicylic+acid%22">Salicylic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Auxin%22">Auxin</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+hormones%22">Plant hormones</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: It is not easy to directly obtain concentrations of auxin (mainly indole-3-acetic acid, or IAA) and other phytohormones in different locations of individual plants although such information is critical for the study of their functions. Here we developed a paper-based analytical device consisting of a disposable working electrode to provide a platform for direct and simultaneous detection of free IAA and salicylic acid (SA) in different parts of pea seedlings with the weights of several milligrams. The double sided conductive carbon tape modified with carbon nanotube was used as the disposable working electrodes after they were treated with oxygen plasma. Different parts of pea seedlings were applied on the surface of working electrodes for direct electrochemical detection of free IAA and SA in paper-based analytical devices. The requirement of solution volumes with only 10 microliters made it possible to quantify free IAA and SA at the level of ng. Our results suggested that large amounts of inherent IAA and SA could be lost because of complicated and time-consuming preparation of samples in traditional gas/liquid chromatography-mass spectrometry methods Our approach not only avoids such steps but also provides concentrations of IAA and SA in different zones of individual pea seedlings. The obtained results could be applied for the study of individual differences and interactions between IAA and SA in development of peas. This strategy not only paved the foundation for further investigation of regulating mechanisms of free IAA and its interaction with other phytohormones but also might provide an alternative approach for detection of free electroactive biomolecules in other living organs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Sensors & Actuators B: Chemical is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=122699289
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.snb.2017.03.025
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 336
    Subjects:
      – SubjectFull: Electrochemical sensors
        Type: general
      – SubjectFull: Acetic acid
        Type: general
      – SubjectFull: Salicylic acid
        Type: general
      – SubjectFull: Auxin
        Type: general
      – SubjectFull: Plant hormones
        Type: general
    Titles:
      – TitleFull: Paper-based analytical devices for direct electrochemical detection of free IAA and SA in plant samples with the weight of several milligrams.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Sun, Li-Jun
      – PersonEntity:
          Name:
            NameFull: Xie, Yuan
      – PersonEntity:
          Name:
            NameFull: Yan, Yong-Feng
      – PersonEntity:
          Name:
            NameFull: Yang, Haibing
      – PersonEntity:
          Name:
            NameFull: Gu, Hai-Ying
      – PersonEntity:
          Name:
            NameFull: Bao, Ning
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 08
              Text: Aug2017
              Type: published
              Y: 2017
          Identifiers:
            – Type: issn-print
              Value: 09254005
          Numbering:
            – Type: volume
              Value: 247
          Titles:
            – TitleFull: Sensors & Actuators B: Chemical
              Type: main
ResultId 1