Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi.
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| Title: | Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi. |
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| Authors: | Studt, Lena1 lena.studt@boku.ac.at, Rösler, Sarah M.1,2, Burkhardt, Immo3, Arndt, Birgit2, Freitag, Michael4, Humpf, Hans ‐ Ulrich2, Dickschat, Jeroen S.3, Tudzynski, Bettina1 |
| Source: | Environmental Microbiology. Nov2016, Vol. 18 Issue 11, p4037-4054. 18p. 1 Diagram, 7 Graphs. |
| Abstract: | Filamentous fungi produce a vast array of secondary metabolites (SMs) and some play a role in agriculture or pharmacology. Sequencing of the rice pathogen Fusarium fujikuroi revealed the presence of far more SM-encoding genes than known products. SM production is energy-consuming and thus tightly regulated, leaving the majority of SM gene clusters silent under laboratory conditions. One important regulatory layer in SM biosynthesis involves histone modifications that render the underlying genes either silent or poised for transcription. Here, we show that the majority of the putative SM gene clusters in F. fujikuroi are located within facultative heterochromatin marked by trimethylated lysine 27 on histone 3 (H3K27me3). Kmt6, the methyltransferase responsible for establishing this histone mark, appears to be essential in this fungus, and knock-down of Kmt6 in the KMT6kd strain shows a drastic phenotype affecting fungal growth and development. Transcription of four so far cryptic and otherwise silent putative SM gene clusters was induced in the KMT6kd strain, in which decreased expression of KMT6 is accompanied by reduced H3K27me3 levels at the respective gene loci and accumulation of novel metabolites. One of the four putative SM gene clusters, named STC5, was analysed in more detail thereby revealing a novel sesquiterpene. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 122718213 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Studt%2C+Lena%22">Studt, Lena</searchLink><relatesTo>1</relatesTo><i> lena.studt@boku.ac.at</i><br /><searchLink fieldCode="AR" term="%22Rösler%2C+Sarah+M%2E%22">Rösler, Sarah M.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Burkhardt%2C+Immo%22">Burkhardt, Immo</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Arndt%2C+Birgit%22">Arndt, Birgit</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Freitag%2C+Michael%22">Freitag, Michael</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Humpf%2C+Hans+‐+Ulrich%22">Humpf, Hans ‐ Ulrich</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Dickschat%2C+Jeroen+S%2E%22">Dickschat, Jeroen S.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Tudzynski%2C+Bettina%22">Tudzynski, Bettina</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. Nov2016, Vol. 18 Issue 11, p4037-4054. 18p. 1 Diagram, 7 Graphs. – Name: Abstract Label: Abstract Group: Ab Data: Filamentous fungi produce a vast array of secondary metabolites (SMs) and some play a role in agriculture or pharmacology. Sequencing of the rice pathogen Fusarium fujikuroi revealed the presence of far more SM-encoding genes than known products. SM production is energy-consuming and thus tightly regulated, leaving the majority of SM gene clusters silent under laboratory conditions. One important regulatory layer in SM biosynthesis involves histone modifications that render the underlying genes either silent or poised for transcription. Here, we show that the majority of the putative SM gene clusters in F. fujikuroi are located within facultative heterochromatin marked by trimethylated lysine 27 on histone 3 (H3K27me3). Kmt6, the methyltransferase responsible for establishing this histone mark, appears to be essential in this fungus, and knock-down of Kmt6 in the KMT6kd strain shows a drastic phenotype affecting fungal growth and development. Transcription of four so far cryptic and otherwise silent putative SM gene clusters was induced in the KMT6kd strain, in which decreased expression of KMT6 is accompanied by reduced H3K27me3 levels at the respective gene loci and accumulation of novel metabolites. One of the four putative SM gene clusters, named STC5, was analysed in more detail thereby revealing a novel sesquiterpene. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/1462-2920.13427 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 18 StartPage: 4037 Titles: – TitleFull: Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Studt, Lena – PersonEntity: Name: NameFull: Rösler, Sarah M. – PersonEntity: Name: NameFull: Burkhardt, Immo – PersonEntity: Name: NameFull: Arndt, Birgit – PersonEntity: Name: NameFull: Freitag, Michael – PersonEntity: Name: NameFull: Humpf, Hans ‐ Ulrich – PersonEntity: Name: NameFull: Dickschat, Jeroen S. – PersonEntity: Name: NameFull: Tudzynski, Bettina IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 14622912 Numbering: – Type: volume Value: 18 – Type: issue Value: 11 Titles: – TitleFull: Environmental Microbiology Type: main |
| ResultId | 1 |
