Horizontal transfer of the blaNDM-1 gene to Pseudomonas aeruginosa and Acinetobacter baumannii in biofilms.
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| Title: | Horizontal transfer of the bla |
|---|---|
| Authors: | Tanner, Windy D.1 windy.tanner@hsc.utah.edu, Atkinson, Robyn M.2, Goel, Ramesh K.3, Toleman, Mark A.4, Benson, Lowell Scott5, Porucznik, Christina A.5, VanDerslice, James A.5 |
| Source: | FEMS Microbiology Letters. Apr2017, Vol. 364 Issue 8, p1-4. 4p. 1 Chart. |
| Subjects: | Genetic transformation, Gram-negative bacteria, Enterobacteriaceae |
| Abstract: | Horizontal gene transfer has contributed to the global spread of the blaNDM-1 gene. Multiple studies have demonstrated plasmid transfer of blaNDM-1 between Gram-negative bacteria, primarily Enterobacteriaceae species, but conjugational transfer of natural blaNDM-1 plasmids from Enterobacteriaceae into Pseudomonas aeruginosa and Acinetobacter baumannii has not previously been shown. As P. aeruginosa and A. baumannii are both typically strong biofilm formers, transfer of natural blaNDM-1 plasmids could potentially occur more readily in this environment. To determine whether natural blaNDM-1 plasmids could transfer to P. aeruginosa or A. baumannii in biofilms, three clinical and environmental Enterobacteriaceae strains carrying NDM-1-encoding plasmids of different incompatibility types were mated with E. coli J53, producing E. coli J53- blaNDM-1 transconjugants. Subsequently, dual-species biofilms were created using the E. coli J53 transconjugants as plasmid donors and either P. aeruginosa or A. baumannii as recipients. Biofilm transfer of NDM-encoding plasmids to P. aeruginosa and A. baumannii was successful from one and two E. coli J53- blaNDM-1 transconjugants, respectively. This demonstrates the potential for the spread of blaNDM-1, genes to P. aeruginosa and A. baumannii in clinical and environmental settings. [ABSTRACT FROM AUTHOR] |
| Copyright of FEMS Microbiology Letters is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 122907386 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Horizontal transfer of the bla<subscript>NDM-1</subscript> gene to Pseudomonas aeruginosa and Acinetobacter baumannii in biofilms. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tanner%2C+Windy+D%2E%22">Tanner, Windy D.</searchLink><relatesTo>1</relatesTo><i> windy.tanner@hsc.utah.edu</i><br /><searchLink fieldCode="AR" term="%22Atkinson%2C+Robyn+M%2E%22">Atkinson, Robyn M.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Goel%2C+Ramesh+K%2E%22">Goel, Ramesh K.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Toleman%2C+Mark+A%2E%22">Toleman, Mark A.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Benson%2C+Lowell+Scott%22">Benson, Lowell Scott</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Porucznik%2C+Christina+A%2E%22">Porucznik, Christina A.</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22VanDerslice%2C+James+A%2E%22">VanDerslice, James A.</searchLink><relatesTo>5</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22FEMS+Microbiology+Letters%22">FEMS Microbiology Letters</searchLink>. Apr2017, Vol. 364 Issue 8, p1-4. 4p. 1 Chart. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Genetic+transformation%22">Genetic transformation</searchLink><br /><searchLink fieldCode="DE" term="%22Gram-negative+bacteria%22">Gram-negative bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Enterobacteriaceae%22">Enterobacteriaceae</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Horizontal gene transfer has contributed to the global spread of the blaNDM-1 gene. Multiple studies have demonstrated plasmid transfer of blaNDM-1 between Gram-negative bacteria, primarily Enterobacteriaceae species, but conjugational transfer of natural blaNDM-1 plasmids from Enterobacteriaceae into Pseudomonas aeruginosa and Acinetobacter baumannii has not previously been shown. As P. aeruginosa and A. baumannii are both typically strong biofilm formers, transfer of natural blaNDM-1 plasmids could potentially occur more readily in this environment. To determine whether natural blaNDM-1 plasmids could transfer to P. aeruginosa or A. baumannii in biofilms, three clinical and environmental Enterobacteriaceae strains carrying NDM-1-encoding plasmids of different incompatibility types were mated with E. coli J53, producing E. coli J53- blaNDM-1 transconjugants. Subsequently, dual-species biofilms were created using the E. coli J53 transconjugants as plasmid donors and either P. aeruginosa or A. baumannii as recipients. Biofilm transfer of NDM-encoding plasmids to P. aeruginosa and A. baumannii was successful from one and two E. coli J53- blaNDM-1 transconjugants, respectively. This demonstrates the potential for the spread of blaNDM-1, genes to P. aeruginosa and A. baumannii in clinical and environmental settings. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of FEMS Microbiology Letters is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1093/femsle/fnx048 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 1 Subjects: – SubjectFull: Genetic transformation Type: general – SubjectFull: Gram-negative bacteria Type: general – SubjectFull: Enterobacteriaceae Type: general Titles: – TitleFull: Horizontal transfer of the blaNDM-1 gene to Pseudomonas aeruginosa and Acinetobacter baumannii in biofilms. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tanner, Windy D. – PersonEntity: Name: NameFull: Atkinson, Robyn M. – PersonEntity: Name: NameFull: Goel, Ramesh K. – PersonEntity: Name: NameFull: Toleman, Mark A. – PersonEntity: Name: NameFull: Benson, Lowell Scott – PersonEntity: Name: NameFull: Porucznik, Christina A. – PersonEntity: Name: NameFull: VanDerslice, James A. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 04 Text: Apr2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 03781097 Numbering: – Type: volume Value: 364 – Type: issue Value: 8 Titles: – TitleFull: FEMS Microbiology Letters Type: main |
| ResultId | 1 |
