Horizontal transfer of the blaNDM-1 gene to Pseudomonas aeruginosa and Acinetobacter baumannii in biofilms.
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| Title: | Horizontal transfer of the bla |
|---|---|
| Authors: | Tanner, Windy D.1 windy.tanner@hsc.utah.edu, Atkinson, Robyn M.2, Goel, Ramesh K.3, Toleman, Mark A.4, Benson, Lowell Scott5, Porucznik, Christina A.5, VanDerslice, James A.5 |
| Source: | FEMS Microbiology Letters. Apr2017, Vol. 364 Issue 8, p1-4. 4p. 1 Chart. |
| Subjects: | Genetic transformation, Gram-negative bacteria, Enterobacteriaceae |
| Abstract: | Horizontal gene transfer has contributed to the global spread of the blaNDM-1 gene. Multiple studies have demonstrated plasmid transfer of blaNDM-1 between Gram-negative bacteria, primarily Enterobacteriaceae species, but conjugational transfer of natural blaNDM-1 plasmids from Enterobacteriaceae into Pseudomonas aeruginosa and Acinetobacter baumannii has not previously been shown. As P. aeruginosa and A. baumannii are both typically strong biofilm formers, transfer of natural blaNDM-1 plasmids could potentially occur more readily in this environment. To determine whether natural blaNDM-1 plasmids could transfer to P. aeruginosa or A. baumannii in biofilms, three clinical and environmental Enterobacteriaceae strains carrying NDM-1-encoding plasmids of different incompatibility types were mated with E. coli J53, producing E. coli J53- blaNDM-1 transconjugants. Subsequently, dual-species biofilms were created using the E. coli J53 transconjugants as plasmid donors and either P. aeruginosa or A. baumannii as recipients. Biofilm transfer of NDM-encoding plasmids to P. aeruginosa and A. baumannii was successful from one and two E. coli J53- blaNDM-1 transconjugants, respectively. This demonstrates the potential for the spread of blaNDM-1, genes to P. aeruginosa and A. baumannii in clinical and environmental settings. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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