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Lipid Layers on Nanoscale Surface Topography: Stability and Effect on Protein Adsorption.

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Title: Lipid Layers on Nanoscale Surface Topography: Stability and Effect on Protein Adsorption.
Authors: Liascukiene, Irma1,2,3, El Kirat, Karim4, Beauvais, Mathieu5, Asadauskas, Svajus J.3, Lambert, Jean-François1,2, Landoulsi, Jessem1,2 jessem.landoulsi@upmc.fr
Source: Langmuir. May2017, Vol. 33 Issue 18, p4414-4425. 12p.
Subjects: Stearic acid, Lipids, Proteins, Phospholipids, Nanostructured materials
Abstract: Herein, we report the coating of a surface with a random nanoscale topography with a lipid film formed by an anchoring stearic acid (SA) monolayer and phospholipid (DPPC) layers. For this purpose, different procedures were used for phospholipid coating, including adsorption from solution, drop deposition, and spin-coating. Our results reveal that the morphology of the obtained lipid films is strongly influenced by the topography of the underlying substrate but also impacted by other factors, including the coating procedure and surface wettability (modulated by the presence of SA). These coated surfaces showed a remarkable antifouling behavior toward proteins, with different yields of repellency (Yrp) depending on the amount/organization of DPPC on the nanostructured substrate. The interaction between the proteins and phospholipids involves a partial detachement of the film. The use of characterization techniques with different charcateristics (accuracy, selectivity, analysis depth) did not reveal any obvious vertical heterogenity of the probed interface, indicating that the lipid film acts as a nonfouling coating on the whole surface, including the outermost part (nanoprotrusions) and deeper regions (valleys). [ABSTRACT FROM AUTHOR]
Copyright of Langmuir is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 123066969
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Lipid Layers on Nanoscale Surface Topography: Stability and Effect on Protein Adsorption.
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  Data: <searchLink fieldCode="JN" term="%22Langmuir%22">Langmuir</searchLink>. May2017, Vol. 33 Issue 18, p4414-4425. 12p.
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  Data: <searchLink fieldCode="DE" term="%22Stearic+acid%22">Stearic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Lipids%22">Lipids</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Phospholipids%22">Phospholipids</searchLink><br /><searchLink fieldCode="DE" term="%22Nanostructured+materials%22">Nanostructured materials</searchLink>
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  Data: Herein, we report the coating of a surface with a random nanoscale topography with a lipid film formed by an anchoring stearic acid (SA) monolayer and phospholipid (DPPC) layers. For this purpose, different procedures were used for phospholipid coating, including adsorption from solution, drop deposition, and spin-coating. Our results reveal that the morphology of the obtained lipid films is strongly influenced by the topography of the underlying substrate but also impacted by other factors, including the coating procedure and surface wettability (modulated by the presence of SA). These coated surfaces showed a remarkable antifouling behavior toward proteins, with different yields of repellency (Yrp) depending on the amount/organization of DPPC on the nanostructured substrate. The interaction between the proteins and phospholipids involves a partial detachement of the film. The use of characterization techniques with different charcateristics (accuracy, selectivity, analysis depth) did not reveal any obvious vertical heterogenity of the probed interface, indicating that the lipid film acts as a nonfouling coating on the whole surface, including the outermost part (nanoprotrusions) and deeper regions (valleys). [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Langmuir is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1021/acs.langmuir.7b00431
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      – Code: eng
        Text: English
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        PageCount: 12
        StartPage: 4414
    Subjects:
      – SubjectFull: Stearic acid
        Type: general
      – SubjectFull: Lipids
        Type: general
      – SubjectFull: Proteins
        Type: general
      – SubjectFull: Phospholipids
        Type: general
      – SubjectFull: Nanostructured materials
        Type: general
    Titles:
      – TitleFull: Lipid Layers on Nanoscale Surface Topography: Stability and Effect on Protein Adsorption.
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            NameFull: Liascukiene, Irma
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            NameFull: El Kirat, Karim
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            NameFull: Lambert, Jean-François
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              M: 05
              Text: May2017
              Type: published
              Y: 2017
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              Value: 33
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