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An overview of advanced technologies for selection of probiotics and their expediency: A review.

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Title: An overview of advanced technologies for selection of probiotics and their expediency: A review.
Authors: Yadav, Ruby1, Shukla, Pratyoosh1
Source: Critical Reviews in Food Science & Nutrition. 2017, Vol. 57 Issue 15, p3233-3242. 10p.
Subjects: Probiotics, Molecular biology, Lactic acid bacteria, Reverse transcriptase polymerase chain reaction, Ribosomal DNA, Bacterial metabolism, Bacteriophage typing, Fermentation, Gram-positive bacteria, Fluorescence in situ hybridization
Abstract: It has become easy to identify and select an appropriate microorganism with the advancement in various molecular biology and analytical techniques. The majority of the novel techniques are being implemented for the identification and characterization of microorganisms used for probiotic application. Standard microbial techniques such as biochemical testing and culture techniques routinely used for probiotic microbes screening, identification and selection. However, these standard techniques may not give complete information on the microbes that can be used for probiotic production. Furthermore, alternative molecular and analytical techniques such as 16S and 23S ribosomal DNA sequencing, RNA analysis by reverse transcriptase (RT-PCR), fluorescent in situ hybridization (FISH), quantitative analysis by real time PCR (RT-PCR or qPCR) and fluorescent activated cell sorting (FACS) are potentially used to confirm and select all types of lactic acid microorganism. All these approaches can be employed in the screening and selection of appropriate lactic acid bacteria which can be potentially used for the production of human use probiotics in large scale fermentation. This review mainly focuses on various tools and techniques used for effective screening and selection of a better candidate bacterium for probiotic applications. [ABSTRACT FROM AUTHOR]
Copyright of Critical Reviews in Food Science & Nutrition is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Data: An overview of advanced technologies for selection of probiotics and their expediency: A review.
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  Data: <searchLink fieldCode="AR" term="%22Yadav%2C+Ruby%22">Yadav, Ruby</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shukla%2C+Pratyoosh%22">Shukla, Pratyoosh</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Critical+Reviews+in+Food+Science+%26+Nutrition%22">Critical Reviews in Food Science & Nutrition</searchLink>. 2017, Vol. 57 Issue 15, p3233-3242. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Probiotics%22">Probiotics</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+biology%22">Molecular biology</searchLink><br /><searchLink fieldCode="DE" term="%22Lactic+acid+bacteria%22">Lactic acid bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Reverse+transcriptase+polymerase+chain+reaction%22">Reverse transcriptase polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Ribosomal+DNA%22">Ribosomal DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+metabolism%22">Bacterial metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteriophage+typing%22">Bacteriophage typing</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Gram-positive+bacteria%22">Gram-positive bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+in+situ+hybridization%22">Fluorescence in situ hybridization</searchLink>
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  Label: Abstract
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  Data: It has become easy to identify and select an appropriate microorganism with the advancement in various molecular biology and analytical techniques. The majority of the novel techniques are being implemented for the identification and characterization of microorganisms used for probiotic application. Standard microbial techniques such as biochemical testing and culture techniques routinely used for probiotic microbes screening, identification and selection. However, these standard techniques may not give complete information on the microbes that can be used for probiotic production. Furthermore, alternative molecular and analytical techniques such as 16S and 23S ribosomal DNA sequencing, RNA analysis by reverse transcriptase (RT-PCR), fluorescent in situ hybridization (FISH), quantitative analysis by real time PCR (RT-PCR or qPCR) and fluorescent activated cell sorting (FACS) are potentially used to confirm and select all types of lactic acid microorganism. All these approaches can be employed in the screening and selection of appropriate lactic acid bacteria which can be potentially used for the production of human use probiotics in large scale fermentation. This review mainly focuses on various tools and techniques used for effective screening and selection of a better candidate bacterium for probiotic applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Critical Reviews in Food Science & Nutrition is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1080/10408398.2015.1108957
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      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 3233
    Subjects:
      – SubjectFull: Probiotics
        Type: general
      – SubjectFull: Molecular biology
        Type: general
      – SubjectFull: Lactic acid bacteria
        Type: general
      – SubjectFull: Reverse transcriptase polymerase chain reaction
        Type: general
      – SubjectFull: Ribosomal DNA
        Type: general
      – SubjectFull: Bacterial metabolism
        Type: general
      – SubjectFull: Bacteriophage typing
        Type: general
      – SubjectFull: Fermentation
        Type: general
      – SubjectFull: Gram-positive bacteria
        Type: general
      – SubjectFull: Fluorescence in situ hybridization
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      – TitleFull: An overview of advanced technologies for selection of probiotics and their expediency: A review.
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              Text: 2017
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