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Bicarbonate utilization by the marine diatom Phaeodactylum tricornutum Bohlin.

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Title: Bicarbonate utilization by the marine diatom Phaeodactylum tricornutum Bohlin.
Authors: Dixon, G. K.1, Merrett, M. J.1
Source: New Phytologist. May88, Vol. 109 Issue 1, p47-51. 5p.
Subjects: Cell membranes, Acetazolamide, Oxygen, Photosynthesis, Carbon, Ions
Abstract: The measurement of inorganic carbon dependent photosynthetic oxygen evolution in air-grown cells of Phaeodactylum tricornutum Bohlin has shown that sodium ions increased the affinity of the cells for bicarbonate. Lithium ions (50 mmol l-1) inhibited bicarbonate-dependent photosynthetic oxygen evolution but this inhibition was overcome at saturating bicarbonate concentrations. Bicarbonate-dependent photosynthetic oxygen evolution was also inhibited by lithium ions in cells of Phaeodactylum grown in high CO2 (5% v/v). Acetazolamide and ethoxyzolamide were potent inhibitors of carbonic anhydrase in cell extracts. At pH 8.0 the K0.5 (CO2) (the concentration of inorganic carbon required for 50% of the maximal rate of photosynthetic oxygen evolution) was increased from 53 to 542 μmol l-1 in the presence of ethoxyzolamide whereas in the presence of acetazolamide the K0.5 (CO2) increased to 130 μmol l-1. In the presence of ethoxyzolamide the internal dissolved inorganic carbon (DIC) concentration was increased but this increase was prevented in the presence of lithium ions when bicarbonate transport into the cell is inhibited. These results are in agreement with bicarbonate transport across the plasmalemma and intracellular carbonic anhydrase increasing the steady-state flux of CO2 from inside the plasmalemma to ribulose-1,5-bisphosphate carboxylase-oxygenase by facilitating the interconversion of HCO3, and CO2. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Bicarbonate utilization by the marine diatom Phaeodactylum tricornutum Bohlin.
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  Data: <searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Acetazolamide%22">Acetazolamide</searchLink><br /><searchLink fieldCode="DE" term="%22Oxygen%22">Oxygen</searchLink><br /><searchLink fieldCode="DE" term="%22Photosynthesis%22">Photosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon%22">Carbon</searchLink><br /><searchLink fieldCode="DE" term="%22Ions%22">Ions</searchLink>
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  Data: The measurement of inorganic carbon dependent photosynthetic oxygen evolution in air-grown cells of Phaeodactylum tricornutum Bohlin has shown that sodium ions increased the affinity of the cells for bicarbonate. Lithium ions (50 mmol l-1) inhibited bicarbonate-dependent photosynthetic oxygen evolution but this inhibition was overcome at saturating bicarbonate concentrations. Bicarbonate-dependent photosynthetic oxygen evolution was also inhibited by lithium ions in cells of Phaeodactylum grown in high CO2 (5% v/v). Acetazolamide and ethoxyzolamide were potent inhibitors of carbonic anhydrase in cell extracts. At pH 8.0 the K0.5 (CO2) (the concentration of inorganic carbon required for 50% of the maximal rate of photosynthetic oxygen evolution) was increased from 53 to 542 μmol l-1 in the presence of ethoxyzolamide whereas in the presence of acetazolamide the K0.5 (CO2) increased to 130 μmol l-1. In the presence of ethoxyzolamide the internal dissolved inorganic carbon (DIC) concentration was increased but this increase was prevented in the presence of lithium ions when bicarbonate transport into the cell is inhibited. These results are in agreement with bicarbonate transport across the plasmalemma and intracellular carbonic anhydrase increasing the steady-state flux of CO2 from inside the plasmalemma to ribulose-1,5-bisphosphate carboxylase-oxygenase by facilitating the interconversion of HCO3, and CO2. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1111/j.1469-8137.1988.tb00217.x
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 5
        StartPage: 47
    Subjects:
      – SubjectFull: Cell membranes
        Type: general
      – SubjectFull: Acetazolamide
        Type: general
      – SubjectFull: Oxygen
        Type: general
      – SubjectFull: Photosynthesis
        Type: general
      – SubjectFull: Carbon
        Type: general
      – SubjectFull: Ions
        Type: general
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      – TitleFull: Bicarbonate utilization by the marine diatom Phaeodactylum tricornutum Bohlin.
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              Text: May88
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              Y: 1988
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