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Engineered polymeric amphiphiles self-assembling into nanostructures and acting as efficient gene and drug carriers.

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Title: Engineered polymeric amphiphiles self-assembling into nanostructures and acting as efficient gene and drug carriers.
Authors: Bansal, Ruby1, Kumar, Pradeep1 pkumar@igib.res.in
Source: Journal of Biomaterials Applications. Jul2017, Vol. 32 Issue 1, p40-53. 14p.
Subjects: Amphiphiles, Nanostructures, Drug carriers, Gene delivery techniques, Drug delivery systems, Physiology
Abstract: Nonviral gene delivery systems are finding widespread use due to their safety, rapid and economical production, and ease of modification. In this work, series of N-alkyl-substituted linear polyethylenimine (CP) polymers have been synthesized, characterized, and investigated about how degree of substitution (hydrophobic–hydrophilic balance) (i.e. N-alkylation) influenced the transfection efficiency. Mobility shift assay demonstrated efficient binding of plasmid DNA (pDNA). Transfection efficiency and cytotoxicity of CP polymers were assessed in vitro, which revealed that all the formulations exhibited higher transfection activity than linear polyethylenimine (lPEI) and commercial transfection reagents, Lipofectamine and Superfect, with negligible toxicity (MTT assay). In the projected series, one of the formulations, CP-3-pDNA complex, displayed the highest transfection efficiency (∼1.6–12 folds vs. lPEI and commercial transfection reagents) and effectively carried GFP-specific siRNA inside the cells as monitored by measuring the suppression in the gene expression of the target gene. Further, flow cytometry experiments confirmed that CP-3-pDNA complex transfected the highest number of cells. Besides, CP-3 was also evaluated in terms of its capability to entrap hydrophobic drug molecules. The results showed that it efficiently encapsulated an anti-cancer drug, etoposide, and released it in a controlled fashion over a period of time. Altogether, the data support that CP-3 is a promising vector for nucleic acid as well as hydrophobic drug delivery. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomaterials Applications is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 123662361
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Engineered polymeric amphiphiles self-assembling into nanostructures and acting as efficient gene and drug carriers.
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  Data: <searchLink fieldCode="AR" term="%22Bansal%2C+Ruby%22">Bansal, Ruby</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kumar%2C+Pradeep%22">Kumar, Pradeep</searchLink><relatesTo>1</relatesTo><i> pkumar@igib.res.in</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomaterials+Applications%22">Journal of Biomaterials Applications</searchLink>. Jul2017, Vol. 32 Issue 1, p40-53. 14p.
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  Data: <searchLink fieldCode="DE" term="%22Amphiphiles%22">Amphiphiles</searchLink><br /><searchLink fieldCode="DE" term="%22Nanostructures%22">Nanostructures</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+carriers%22">Drug carriers</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+delivery+techniques%22">Gene delivery techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+delivery+systems%22">Drug delivery systems</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink>
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  Label: Abstract
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  Data: Nonviral gene delivery systems are finding widespread use due to their safety, rapid and economical production, and ease of modification. In this work, series of N-alkyl-substituted linear polyethylenimine (CP) polymers have been synthesized, characterized, and investigated about how degree of substitution (hydrophobic–hydrophilic balance) (i.e. N-alkylation) influenced the transfection efficiency. Mobility shift assay demonstrated efficient binding of plasmid DNA (pDNA). Transfection efficiency and cytotoxicity of CP polymers were assessed in vitro, which revealed that all the formulations exhibited higher transfection activity than linear polyethylenimine (lPEI) and commercial transfection reagents, Lipofectamine and Superfect, with negligible toxicity (MTT assay). In the projected series, one of the formulations, CP-3-pDNA complex, displayed the highest transfection efficiency (∼1.6–12 folds vs. lPEI and commercial transfection reagents) and effectively carried GFP-specific siRNA inside the cells as monitored by measuring the suppression in the gene expression of the target gene. Further, flow cytometry experiments confirmed that CP-3-pDNA complex transfected the highest number of cells. Besides, CP-3 was also evaluated in terms of its capability to entrap hydrophobic drug molecules. The results showed that it efficiently encapsulated an anti-cancer drug, etoposide, and released it in a controlled fashion over a period of time. Altogether, the data support that CP-3 is a promising vector for nucleic acid as well as hydrophobic drug delivery. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biomaterials Applications is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1177/0885328217710125
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      – Code: eng
        Text: English
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        PageCount: 14
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      – SubjectFull: Amphiphiles
        Type: general
      – SubjectFull: Nanostructures
        Type: general
      – SubjectFull: Drug carriers
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      – SubjectFull: Gene delivery techniques
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      – SubjectFull: Drug delivery systems
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      – SubjectFull: Physiology
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      – TitleFull: Engineered polymeric amphiphiles self-assembling into nanostructures and acting as efficient gene and drug carriers.
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              M: 07
              Text: Jul2017
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              Y: 2017
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