GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation.
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| Title: | GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation. |
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| Authors: | Cai, Zhaoming1,2, Wang, Youning1, Zhu, Lin1, Tian, Yinping3, Chen, Liang3, Sun, Zhengxi3,4, Ullah, Ihteram1,3, Li, Xia1 xli@mail.hzau.edu.cn |
| Source: | New Phytologist. Jul2017, Vol. 215 Issue 2, p672-686. 15p. |
| Subjects: | Auxin, Legumes, Soybean, Genes, Genetic overexpression |
| Abstract: | Auxins play important roles in the nodulation of legumes. However, the mechanism by which auxin signaling regulates root nodulation is largely unknown. In particular, the role of auxin receptors and their regulation in determinate nodule development remains elusive., We checked the expression pattern of the auxin receptor GmTIR1/ GmAFB3 genes in soybean. We analyzed the functions of GmTIR1/AFB3 in the regulation of rhizobial infection and nodule number, and also tested the functions of miR393 during nodulation and its relationship with GmTIR1/AFB3., The results showed that GmTIR1 and GmAFB3 genes exhibit diverse expression patterns during nodulation and overexpression of GmTIR1 genes significantly increased inflection foci and eventual nodule number. GmTIR1/AFB3 genes were post-transcriptionally cleaved by miR393 family and knock-down of the miR393 family members significantly increased rhizobial infection and the nodule number. Overexpression of the mutated form of GmTIR1C at the miR393 cleavage site that is resistant to miR393 cleavage led to a further increase in the number of infection foci and nodules, suggesting that miR393s modulate nodulation by directly targeting GmTIR1C., This study demonstrated that GmTIR1- and GmAFB3-mediated auxin signaling, that is spatio-temporally regulated by miR393, plays a crucial role in determinate nodule development in soybean. [ABSTRACT FROM AUTHOR] |
| Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 123692730 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Cai%2C+Zhaoming%22">Cai, Zhaoming</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Youning%22">Wang, Youning</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhu%2C+Lin%22">Zhu, Lin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Tian%2C+Yinping%22">Tian, Yinping</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Liang%22">Chen, Liang</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Sun%2C+Zhengxi%22">Sun, Zhengxi</searchLink><relatesTo>3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Ullah%2C+Ihteram%22">Ullah, Ihteram</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Xia%22">Li, Xia</searchLink><relatesTo>1</relatesTo><i> xli@mail.hzau.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Jul2017, Vol. 215 Issue 2, p672-686. 15p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Auxin%22">Auxin</searchLink><br /><searchLink fieldCode="DE" term="%22Legumes%22">Legumes</searchLink><br /><searchLink fieldCode="DE" term="%22Soybean%22">Soybean</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+overexpression%22">Genetic overexpression</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Auxins play important roles in the nodulation of legumes. However, the mechanism by which auxin signaling regulates root nodulation is largely unknown. In particular, the role of auxin receptors and their regulation in determinate nodule development remains elusive., We checked the expression pattern of the auxin receptor GmTIR1/ GmAFB3 genes in soybean. We analyzed the functions of GmTIR1/AFB3 in the regulation of rhizobial infection and nodule number, and also tested the functions of miR393 during nodulation and its relationship with GmTIR1/AFB3., The results showed that GmTIR1 and GmAFB3 genes exhibit diverse expression patterns during nodulation and overexpression of GmTIR1 genes significantly increased inflection foci and eventual nodule number. GmTIR1/AFB3 genes were post-transcriptionally cleaved by miR393 family and knock-down of the miR393 family members significantly increased rhizobial infection and the nodule number. Overexpression of the mutated form of GmTIR1C at the miR393 cleavage site that is resistant to miR393 cleavage led to a further increase in the number of infection foci and nodules, suggesting that miR393s modulate nodulation by directly targeting GmTIR1C., This study demonstrated that GmTIR1- and GmAFB3-mediated auxin signaling, that is spatio-temporally regulated by miR393, plays a crucial role in determinate nodule development in soybean. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/nph.14632 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 672 Subjects: – SubjectFull: Auxin Type: general – SubjectFull: Legumes Type: general – SubjectFull: Soybean Type: general – SubjectFull: Genes Type: general – SubjectFull: Genetic overexpression Type: general Titles: – TitleFull: GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Cai, Zhaoming – PersonEntity: Name: NameFull: Wang, Youning – PersonEntity: Name: NameFull: Zhu, Lin – PersonEntity: Name: NameFull: Tian, Yinping – PersonEntity: Name: NameFull: Chen, Liang – PersonEntity: Name: NameFull: Sun, Zhengxi – PersonEntity: Name: NameFull: Ullah, Ihteram – PersonEntity: Name: NameFull: Li, Xia IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 07 Text: Jul2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 0028646X Numbering: – Type: volume Value: 215 – Type: issue Value: 2 Titles: – TitleFull: New Phytologist Type: main |
| ResultId | 1 |
