Ultrastructure of ellipsoidal magnetotactic multicellular prokaryotes depicts their complex assemblage and cellular polarity in the context of magnetotaxis.
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| Title: | Ultrastructure of ellipsoidal magnetotactic multicellular prokaryotes depicts their complex assemblage and cellular polarity in the context of magnetotaxis. |
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| Authors: | Leão, Pedro1, Chen, Yi ‐ Ran2,3, Abreu, Fernanda1, Wang, Mingling2, Zhang, Wei ‐ Jia3,4, Zhou, Ke2, Xiao, Tian2,3, Wu, Long ‐ Fei3,5 wu@imm.cnrs.fr, Lins, Ulysses1 ulins@micro.ufrj.br |
| Source: | Environmental Microbiology. Jun2017, Vol. 19 Issue 6, p2151-2163. 13p. |
| Subjects: | Magnetotactic bacteria, Prokaryotes, Gram-negative bacteria, Magnetosomes, Focused ion beams, Scanning electron microscopy |
| Abstract: | Magnetotactic multicellular prokaryotes (MMPs) consist of unique microorganisms formed by genetically identical Gram-negative bacterial that live as a single individual capable of producing magnetic nanoparticles called magnetosomes. Two distinct morphotypes of MMPs are known: spherical MMPs (sMMPs) and ellipsoidal MMPs (eMMPs). sMMPs have been extensively characterized, but less information exists for eMMPs. Here, we report the ultrastructure and organization as well as gene clusters responsible for magnetosome and flagella biosynthesis in the magnetite magnetosome producer eMMP Candidatus Magnetananas rongchenensis. Transmission electron microscopy and focused ion beam scanning electron microscopy (FIB-SEM) 3D reconstruction reveal that cells with a conspicuous core-periphery polarity were organized around a central space. Magnetosomes were organized in multiple chains aligned along the periphery of each cell. In the partially sequenced genome, magnetite-related mamAB gene and mad gene clusters were identified. Two cell morphologies were detected: irregular elliptical conical 'frustum-like' (IECF) cells and H-shaped cells. IECF cells merge to form H-shaped cells indicating a more complex structure and possibly a distinct evolutionary position of eMMPs when compared with sMMPs considering multicellularity. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 123801648 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Ultrastructure of ellipsoidal magnetotactic multicellular prokaryotes depicts their complex assemblage and cellular polarity in the context of magnetotaxis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Leão%2C+Pedro%22">Leão, Pedro</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Yi+‐+Ran%22">Chen, Yi ‐ Ran</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Abreu%2C+Fernanda%22">Abreu, Fernanda</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Mingling%22">Wang, Mingling</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Wei+‐+Jia%22">Zhang, Wei ‐ Jia</searchLink><relatesTo>3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Ke%22">Zhou, Ke</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xiao%2C+Tian%22">Xiao, Tian</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Long+‐+Fei%22">Wu, Long ‐ Fei</searchLink><relatesTo>3,5</relatesTo><i> wu@imm.cnrs.fr</i><br /><searchLink fieldCode="AR" term="%22Lins%2C+Ulysses%22">Lins, Ulysses</searchLink><relatesTo>1</relatesTo><i> ulins@micro.ufrj.br</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. Jun2017, Vol. 19 Issue 6, p2151-2163. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Magnetotactic+bacteria%22">Magnetotactic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Prokaryotes%22">Prokaryotes</searchLink><br /><searchLink fieldCode="DE" term="%22Gram-negative+bacteria%22">Gram-negative bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetosomes%22">Magnetosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Focused+ion+beams%22">Focused ion beams</searchLink><br /><searchLink fieldCode="DE" term="%22Scanning+electron+microscopy%22">Scanning electron microscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Magnetotactic multicellular prokaryotes (MMPs) consist of unique microorganisms formed by genetically identical Gram-negative bacterial that live as a single individual capable of producing magnetic nanoparticles called magnetosomes. Two distinct morphotypes of MMPs are known: spherical MMPs (sMMPs) and ellipsoidal MMPs (eMMPs). sMMPs have been extensively characterized, but less information exists for eMMPs. Here, we report the ultrastructure and organization as well as gene clusters responsible for magnetosome and flagella biosynthesis in the magnetite magnetosome producer eMMP Candidatus Magnetananas rongchenensis. Transmission electron microscopy and focused ion beam scanning electron microscopy (FIB-SEM) 3D reconstruction reveal that cells with a conspicuous core-periphery polarity were organized around a central space. Magnetosomes were organized in multiple chains aligned along the periphery of each cell. In the partially sequenced genome, magnetite-related mamAB gene and mad gene clusters were identified. Two cell morphologies were detected: irregular elliptical conical 'frustum-like' (IECF) cells and H-shaped cells. IECF cells merge to form H-shaped cells indicating a more complex structure and possibly a distinct evolutionary position of eMMPs when compared with sMMPs considering multicellularity. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/1462-2920.13677 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 2151 Subjects: – SubjectFull: Magnetotactic bacteria Type: general – SubjectFull: Prokaryotes Type: general – SubjectFull: Gram-negative bacteria Type: general – SubjectFull: Magnetosomes Type: general – SubjectFull: Focused ion beams Type: general – SubjectFull: Scanning electron microscopy Type: general Titles: – TitleFull: Ultrastructure of ellipsoidal magnetotactic multicellular prokaryotes depicts their complex assemblage and cellular polarity in the context of magnetotaxis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Leão, Pedro – PersonEntity: Name: NameFull: Chen, Yi ‐ Ran – PersonEntity: Name: NameFull: Abreu, Fernanda – PersonEntity: Name: NameFull: Wang, Mingling – PersonEntity: Name: NameFull: Zhang, Wei ‐ Jia – PersonEntity: Name: NameFull: Zhou, Ke – PersonEntity: Name: NameFull: Xiao, Tian – PersonEntity: Name: NameFull: Wu, Long ‐ Fei – PersonEntity: Name: NameFull: Lins, Ulysses IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 14622912 Numbering: – Type: volume Value: 19 – Type: issue Value: 6 Titles: – TitleFull: Environmental Microbiology Type: main |
| ResultId | 1 |
