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In situ electrochemical enrichment and isolation of a magnetite-reducing bacterium from a high pH serpentinizing spring.

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Title: In situ electrochemical enrichment and isolation of a magnetite-reducing bacterium from a high pH serpentinizing spring.
Authors: Rowe, Annette R.1 annettrr@usc.edu, Yoshimura, Miho2, LaRowe, Doug E.1, Bird, Lina J.1, Amend, Jan P.1,3, Hashimoto, Kazuhito4, Nealson, Kenneth H.1, Okamoto, Akihiro4 okamoto.akihiro@nims.go.jp
Source: Environmental Microbiology. Jun2017, Vol. 19 Issue 6, p2272-2285. 14p.
Subjects: Magnetite, Charge exchange, Microorganisms, Proteobacteria, Carbon fixation
Abstract: Serpentinization is a geologic process that produces highly reduced, hydrogen-rich fluids that support microbial communities under high pH conditions. We investigated the activity of microbes capable of extracellular electron transfer in a terrestrial serpentinizing system known as 'The Cedars'. Measuring current generation with an on-site two-electrode system, we observed daily oscillations in current with the current maxima and minima occurring during daylight hours. Distinct members of the microbial community were enriched. Current generation in lab-scale electrochemical reactors did not oscillate, but was correlated with carbohydrate amendment in Cedars-specific minimal media. Gammaproteobacteria and Firmicutes were consistently enriched from lab electrochemical systems on δ-MnO2 and amorphous Fe(OH)3 at pH 11. However, isolation of an electrogenic strain proved difficult as transfer cultures failed to grow after multiple rounds of media transfer. Lowering the bulk pH in the media allowed us to isolate a Firmicutes strain (Paenibacillus sp.). This strain was capable of electrode and mineral reduction (including magnetite) at pH 9. This report provides evidence of the in situ activity of microbes using extracellular substrates as sinks for electrons at The Cedars, but also highlights the potential importance of community dynamics for supporting microbial life through either carbon fixation, and/or moderating pH stress. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 123801656
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  Data: In situ electrochemical enrichment and isolation of a magnetite-reducing bacterium from a high pH serpentinizing spring.
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  Data: <searchLink fieldCode="AR" term="%22Rowe%2C+Annette+R%2E%22">Rowe, Annette R.</searchLink><relatesTo>1</relatesTo><i> annettrr@usc.edu</i><br /><searchLink fieldCode="AR" term="%22Yoshimura%2C+Miho%22">Yoshimura, Miho</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22LaRowe%2C+Doug+E%2E%22">LaRowe, Doug E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bird%2C+Lina+J%2E%22">Bird, Lina J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Amend%2C+Jan+P%2E%22">Amend, Jan P.</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Hashimoto%2C+Kazuhito%22">Hashimoto, Kazuhito</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Nealson%2C+Kenneth+H%2E%22">Nealson, Kenneth H.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Okamoto%2C+Akihiro%22">Okamoto, Akihiro</searchLink><relatesTo>4</relatesTo><i> okamoto.akihiro@nims.go.jp</i>
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  Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. Jun2017, Vol. 19 Issue 6, p2272-2285. 14p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Magnetite%22">Magnetite</searchLink><br /><searchLink fieldCode="DE" term="%22Charge+exchange%22">Charge exchange</searchLink><br /><searchLink fieldCode="DE" term="%22Microorganisms%22">Microorganisms</searchLink><br /><searchLink fieldCode="DE" term="%22Proteobacteria%22">Proteobacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+fixation%22">Carbon fixation</searchLink>
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  Label: Abstract
  Group: Ab
  Data: Serpentinization is a geologic process that produces highly reduced, hydrogen-rich fluids that support microbial communities under high pH conditions. We investigated the activity of microbes capable of extracellular electron transfer in a terrestrial serpentinizing system known as 'The Cedars'. Measuring current generation with an on-site two-electrode system, we observed daily oscillations in current with the current maxima and minima occurring during daylight hours. Distinct members of the microbial community were enriched. Current generation in lab-scale electrochemical reactors did not oscillate, but was correlated with carbohydrate amendment in Cedars-specific minimal media. Gammaproteobacteria and Firmicutes were consistently enriched from lab electrochemical systems on δ-MnO2 and amorphous Fe(OH)3 at pH 11. However, isolation of an electrogenic strain proved difficult as transfer cultures failed to grow after multiple rounds of media transfer. Lowering the bulk pH in the media allowed us to isolate a Firmicutes strain (Paenibacillus sp.). This strain was capable of electrode and mineral reduction (including magnetite) at pH 9. This report provides evidence of the in situ activity of microbes using extracellular substrates as sinks for electrons at The Cedars, but also highlights the potential importance of community dynamics for supporting microbial life through either carbon fixation, and/or moderating pH stress. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/1462-2920.13723
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        Text: English
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        PageCount: 14
        StartPage: 2272
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        Type: general
      – SubjectFull: Charge exchange
        Type: general
      – SubjectFull: Microorganisms
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      – SubjectFull: Proteobacteria
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      – SubjectFull: Carbon fixation
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      – TitleFull: In situ electrochemical enrichment and isolation of a magnetite-reducing bacterium from a high pH serpentinizing spring.
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            NameFull: Rowe, Annette R.
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              Text: Jun2017
              Type: published
              Y: 2017
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