Development of a Three-Dimensional Adipose Tissue Model for Studying Embryonic Exposures to Obesogenic Chemicals.
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| Title: | Development of a Three-Dimensional Adipose Tissue Model for Studying Embryonic Exposures to Obesogenic Chemicals. |
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| Authors: | Wang, Rebecca1, Abbott, Rosalyn1, Zieba, Adam1, Borowsky, Francis1, Kaplan, David1 David.Kaplan@tufts.edu |
| Source: | Annals of Biomedical Engineering. Jul2017, Vol. 45 Issue 7, p1807-1818. 12p. |
| Subjects: | Obesity, Fat cells, Bisphenol A, Stem cells, Fatty acids |
| Abstract: | Obesity is a rising issue especially in the United States that can lead to heart problems, type II diabetes, and respiratory problems. Since the 1970s, obesity rates in the United States have more than doubled in adults and children. Recent evidence suggests that exposure to certain chemicals, termed 'obesogens,' in utero may alter metabolic processes, predisposing individuals to weight gain. There is a need to develop a three-dimensional human tissue system that is able to model the effects of obesogens in vitro in order to better understand the impact of obesogens on early development. Human embryonic-derived stem cells in three-dimensional collagen embedded silk scaffolds were exposed to three different obesogens: Bisphenol A (BPA), Bisphenol S (BPS), and Tributyltin (TBT). The exposed tissues accumulated triglycerides and increased expression of adipogenic genes (Perilipin (PLIN1), peroxisome proliferator-activated receptor gamma (PPARy), fatty acid binding protein 4 (FABP4)) compared to equivalent control cultures with no obesogen exposure. These cultures were also compared to human adult stem cell cultures, which did not respond the same upon addition of obesogens. These results demonstrate the successful development of a representative tissue model of in utero obesogen exposures. This tissue system could be used to determine mechanisms of action of current obesogens and to screen other potential obesogens. [ABSTRACT FROM AUTHOR] |
| Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 123821243 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development of a Three-Dimensional Adipose Tissue Model for Studying Embryonic Exposures to Obesogenic Chemicals. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Rebecca%22">Wang, Rebecca</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Abbott%2C+Rosalyn%22">Abbott, Rosalyn</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zieba%2C+Adam%22">Zieba, Adam</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Borowsky%2C+Francis%22">Borowsky, Francis</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kaplan%2C+David%22">Kaplan, David</searchLink><relatesTo>1</relatesTo><i> David.Kaplan@tufts.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Annals+of+Biomedical+Engineering%22">Annals of Biomedical Engineering</searchLink>. Jul2017, Vol. 45 Issue 7, p1807-1818. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Obesity%22">Obesity</searchLink><br /><searchLink fieldCode="DE" term="%22Fat+cells%22">Fat cells</searchLink><br /><searchLink fieldCode="DE" term="%22Bisphenol+A%22">Bisphenol A</searchLink><br /><searchLink fieldCode="DE" term="%22Stem+cells%22">Stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Fatty+acids%22">Fatty acids</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Obesity is a rising issue especially in the United States that can lead to heart problems, type II diabetes, and respiratory problems. Since the 1970s, obesity rates in the United States have more than doubled in adults and children. Recent evidence suggests that exposure to certain chemicals, termed 'obesogens,' in utero may alter metabolic processes, predisposing individuals to weight gain. There is a need to develop a three-dimensional human tissue system that is able to model the effects of obesogens in vitro in order to better understand the impact of obesogens on early development. Human embryonic-derived stem cells in three-dimensional collagen embedded silk scaffolds were exposed to three different obesogens: Bisphenol A (BPA), Bisphenol S (BPS), and Tributyltin (TBT). The exposed tissues accumulated triglycerides and increased expression of adipogenic genes (Perilipin (PLIN1), peroxisome proliferator-activated receptor gamma (PPARy), fatty acid binding protein 4 (FABP4)) compared to equivalent control cultures with no obesogen exposure. These cultures were also compared to human adult stem cell cultures, which did not respond the same upon addition of obesogens. These results demonstrate the successful development of a representative tissue model of in utero obesogen exposures. This tissue system could be used to determine mechanisms of action of current obesogens and to screen other potential obesogens. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10439-016-1752-x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1807 Subjects: – SubjectFull: Obesity Type: general – SubjectFull: Fat cells Type: general – SubjectFull: Bisphenol A Type: general – SubjectFull: Stem cells Type: general – SubjectFull: Fatty acids Type: general Titles: – TitleFull: Development of a Three-Dimensional Adipose Tissue Model for Studying Embryonic Exposures to Obesogenic Chemicals. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Rebecca – PersonEntity: Name: NameFull: Abbott, Rosalyn – PersonEntity: Name: NameFull: Zieba, Adam – PersonEntity: Name: NameFull: Borowsky, Francis – PersonEntity: Name: NameFull: Kaplan, David IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 00906964 Numbering: – Type: volume Value: 45 – Type: issue Value: 7 Titles: – TitleFull: Annals of Biomedical Engineering Type: main |
| ResultId | 1 |
