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Calcification and utilization of inorganic carbon by the coccolithophorid Emiliania huxleyi Lohmann.

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Title: Calcification and utilization of inorganic carbon by the coccolithophorid Emiliania huxleyi Lohmann.
Authors: Nimer, N. A.1, Merrett, M. J.1
Source: New Phytologist. Jun92, Vol. 121 Issue 2, p173-177. 5p.
Subjects: Carbon, Light elements, Photosynthetic oxygen evolution, Cells, Metabolism, Biology
Abstract: The relationship between inorganic-carbon dependent photosynthetic oxygen evolution and calcification was investigated in high- and low-calcifying strains of Emiliania huxleyi showing a ten-fold difference in calcification rate. Unlike the low-calcifying strain calcifying cultures showed a four-fold increase in inorganic carbon (1 mM) dependent photosynthetic oxygen over the pH range 5-8.3 resulting in a 20 fold difference in photosynthetic rate between the two strains at pH 8.3. Calcifying cells have a high affinity for HCO3-, the concentration of dissolved inorganic carbon [DICJ required for half-maximal rate of photosynthetic O2 evolution (K0.5 [DIC]) being 200 μM at pH 8.3. In mid-exponential phase cultures the stoichiometry between 11CO2 fixation and calcification was 1:1 so it is likely that the high photosynthetic rate at pH 8.3 is sustained by 14CO2 released from H 14CO3 during calcification. Measurement of bicarbonate transport by the silicone-oil-layer centrifugal filtering technique demonstrated a rapid uptake and achievement of equilibrium (less than 3 s) between the intracellular and external inorganic carbon concentrations in low and high-calcifying cells. Subsequent metabolism of the 14C intracellular inorganic carbon pool did not occur in low-calcifying cells suggesting the block in calcification occurs either in transport into or within the coccolith vesicle. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 12415370
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  Data: Calcification and utilization of inorganic carbon by the coccolithophorid Emiliania huxleyi Lohmann.
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  Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Jun92, Vol. 121 Issue 2, p173-177. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Carbon%22">Carbon</searchLink><br /><searchLink fieldCode="DE" term="%22Light+elements%22">Light elements</searchLink><br /><searchLink fieldCode="DE" term="%22Photosynthetic+oxygen+evolution%22">Photosynthetic oxygen evolution</searchLink><br /><searchLink fieldCode="DE" term="%22Cells%22">Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Biology%22">Biology</searchLink>
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  Data: The relationship between inorganic-carbon dependent photosynthetic oxygen evolution and calcification was investigated in high- and low-calcifying strains of <em>Emiliania huxleyi</em> showing a ten-fold difference in calcification rate. Unlike the low-calcifying strain calcifying cultures showed a four-fold increase in inorganic carbon (1 mM) dependent photosynthetic oxygen over the pH range 5-8.3 resulting in a 20 fold difference in photosynthetic rate between the two strains at pH 8.3. Calcifying cells have a high affinity for HCO3-, the concentration of dissolved inorganic carbon [DICJ required for half-maximal rate of photosynthetic O2 evolution (K0.5 [DIC]) being 200 μM at pH 8.3. In mid-exponential phase cultures the stoichiometry between 11CO2 fixation and calcification was 1:1 so it is likely that the high photosynthetic rate at pH 8.3 is sustained by 14CO2 released from H 14CO3 during calcification. Measurement of bicarbonate transport by the silicone-oil-layer centrifugal filtering technique demonstrated a rapid uptake and achievement of equilibrium (less than 3 s) between the intracellular and external inorganic carbon concentrations in low and high-calcifying cells. Subsequent metabolism of the 14C intracellular inorganic carbon pool did not occur in low-calcifying cells suggesting the block in calcification occurs either in transport into or within the coccolith vesicle. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/j.1469-8137.1992.tb01102.x
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      – Code: eng
        Text: English
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        PageCount: 5
        StartPage: 173
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      – SubjectFull: Carbon
        Type: general
      – SubjectFull: Light elements
        Type: general
      – SubjectFull: Photosynthetic oxygen evolution
        Type: general
      – SubjectFull: Cells
        Type: general
      – SubjectFull: Metabolism
        Type: general
      – SubjectFull: Biology
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      – TitleFull: Calcification and utilization of inorganic carbon by the coccolithophorid Emiliania huxleyi Lohmann.
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              Text: Jun92
              Type: published
              Y: 1992
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