Identification by serological proteome analysis of paramyosin as prominent allergen in dust mite allergy.
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| Title: | Identification by serological proteome analysis of paramyosin as prominent allergen in dust mite allergy. |
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| Authors: | Conti, Antonio1, Burastero, Giulia J.1, Suli, Claudia2, Banerjee, Srinita3, Vrtala, Susanne3, Alessio, Massimo1 alessio.massimo@hsr.it, Burastero, Samuele E.2 burastero.samuele@hsr.it |
| Source: | Journal of Proteomics. Aug2017, Vol. 166, p19-26. 8p. |
| Subjects: | Immunoglobulin E, Serum, Allergens, House dust mites, Allergy diagnosis |
| Abstract: | Component-resolved diagnosis (CRD) of IgE-mediated hypersensitivities is challenged by the possibility that single patients are sensitized to components not commercially available to the clinical lab. Here, we studied a patient with positive extract-based diagnosis of house dust mite (HDM) allergy based on routine in vivo (prick test) and in vitro (serum specific IgE) tests, whose serum scored negative for IgE to the three recombinant allergens routinely used in CRD (group 1 allergens, group 2 allergens and tropomyosin). By means of serological proteome analysis via two-dimensional gel electrophoresis combined with immunoblotting and mass spectrometry, paramyosin (group 11 allergen: Der f 11 and Der p 11) was identified as the allergen component recognized by serum IgE from this patient in a raw allergen extract. Nine patients (64%) had IgE to Der p 11 in a group of 14 HDM allergic patients. Biological significance Our results add up to previous reports indicating that paramyosin is a clinically relevant HDM allergen and highlight that it can represent, in some patients, the first sensitizing component of this allergen source. This suggests that, at the moment, the use of allergen extract for the purpose of measuring IgE reactivity cannot be replaced by component resolved diagnosis and that group 11 allergens should be included among allergen components routinely tested in the clinical laboratory. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 124630189 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Identification by serological proteome analysis of paramyosin as prominent allergen in dust mite allergy. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Conti%2C+Antonio%22">Conti, Antonio</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Burastero%2C+Giulia+J%2E%22">Burastero, Giulia J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Suli%2C+Claudia%22">Suli, Claudia</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Banerjee%2C+Srinita%22">Banerjee, Srinita</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Vrtala%2C+Susanne%22">Vrtala, Susanne</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Alessio%2C+Massimo%22">Alessio, Massimo</searchLink><relatesTo>1</relatesTo><i> alessio.massimo@hsr.it</i><br /><searchLink fieldCode="AR" term="%22Burastero%2C+Samuele+E%2E%22">Burastero, Samuele E.</searchLink><relatesTo>2</relatesTo><i> burastero.samuele@hsr.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Proteomics%22">Journal of Proteomics</searchLink>. Aug2017, Vol. 166, p19-26. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Immunoglobulin+E%22">Immunoglobulin E</searchLink><br /><searchLink fieldCode="DE" term="%22Serum%22">Serum</searchLink><br /><searchLink fieldCode="DE" term="%22Allergens%22">Allergens</searchLink><br /><searchLink fieldCode="DE" term="%22House+dust+mites%22">House dust mites</searchLink><br /><searchLink fieldCode="DE" term="%22Allergy+diagnosis%22">Allergy diagnosis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Component-resolved diagnosis (CRD) of IgE-mediated hypersensitivities is challenged by the possibility that single patients are sensitized to components not commercially available to the clinical lab. Here, we studied a patient with positive extract-based diagnosis of house dust mite (HDM) allergy based on routine in vivo (prick test) and in vitro (serum specific IgE) tests, whose serum scored negative for IgE to the three recombinant allergens routinely used in CRD (group 1 allergens, group 2 allergens and tropomyosin). By means of serological proteome analysis via two-dimensional gel electrophoresis combined with immunoblotting and mass spectrometry, paramyosin (group 11 allergen: Der f 11 and Der p 11) was identified as the allergen component recognized by serum IgE from this patient in a raw allergen extract. Nine patients (64%) had IgE to Der p 11 in a group of 14 HDM allergic patients. Biological significance Our results add up to previous reports indicating that paramyosin is a clinically relevant HDM allergen and highlight that it can represent, in some patients, the first sensitizing component of this allergen source. This suggests that, at the moment, the use of allergen extract for the purpose of measuring IgE reactivity cannot be replaced by component resolved diagnosis and that group 11 allergens should be included among allergen components routinely tested in the clinical laboratory. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jprot.2017.06.024 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 19 Subjects: – SubjectFull: Immunoglobulin E Type: general – SubjectFull: Serum Type: general – SubjectFull: Allergens Type: general – SubjectFull: House dust mites Type: general – SubjectFull: Allergy diagnosis Type: general Titles: – TitleFull: Identification by serological proteome analysis of paramyosin as prominent allergen in dust mite allergy. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Conti, Antonio – PersonEntity: Name: NameFull: Burastero, Giulia J. – PersonEntity: Name: NameFull: Suli, Claudia – PersonEntity: Name: NameFull: Banerjee, Srinita – PersonEntity: Name: NameFull: Vrtala, Susanne – PersonEntity: Name: NameFull: Alessio, Massimo – PersonEntity: Name: NameFull: Burastero, Samuele E. IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 08 Text: Aug2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 18743919 Numbering: – Type: volume Value: 166 Titles: – TitleFull: Journal of Proteomics Type: main |
| ResultId | 1 |
