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Cryopreservation of Luciola praeusta Kiesenwetter (Coleoptera: Lampyridae) embryos by vitrification.

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Title: Cryopreservation of Luciola praeusta Kiesenwetter (Coleoptera: Lampyridae) embryos by vitrification.
Authors: Li, Kang1, Chen, Xuanli2 drchenxuanli@hotmail.com, Song, Xiaoling3, Wu, Xi1, Xian, Yi4
Source: Cryobiology. Oct2017, Vol. 78, p101-105. 5p.
Subjects: Fireflies, Insect embryos, Povidone, Cryoprotective agents, Ethylene glycol
Abstract: There is an urgent need to preserve the ever-decreasing number of different species of fireflies all over the world. We sought to develop a vitrification procedure to cryopreserve the firefly embryos. The late stages of Luciola praeusta Kiesenwetter embryos were collected. Several impermeable and permeable protectants with various concentrations in different mediums (TNM-FH insect medium, Grace's medium, Dulbecco's Modification of Eagle's Medium (DMEM) and Dulbecco's Phosphate-Buffered Saline (DPBS)) were used. Embryos culturing in TNM-FH medium yielded the highest survival rate of 75.3 ± 3.6%. One-step, two-step and three-step methods were used in equilibrium procedure respectively. The highest survival rate (73.7% ±3.2%) occurred in embryos treated by three-step method ((1.5 M ethylene glycol (EG) + 2 M EG plus 8% polyvinylpyrrolidone (PVP) + 3 M EG, 8% PVP and 15% trehalose). Additionally, embryos exposed to 0.5 M trehalose presented a significantly higher survival rate (71.8 ± 2.7%) than embryos preserved in 0.5 M sucrose. [ABSTRACT FROM AUTHOR]
Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
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  Label: Title
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  Data: Cryopreservation of Luciola praeusta Kiesenwetter (Coleoptera: Lampyridae) embryos by vitrification.
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Kang%22">Li, Kang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen%2C+Xuanli%22">Chen, Xuanli</searchLink><relatesTo>2</relatesTo><i> drchenxuanli@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Song%2C+Xiaoling%22">Song, Xiaoling</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Xi%22">Wu, Xi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xian%2C+Yi%22">Xian, Yi</searchLink><relatesTo>4</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Cryobiology%22">Cryobiology</searchLink>. Oct2017, Vol. 78, p101-105. 5p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Fireflies%22">Fireflies</searchLink><br /><searchLink fieldCode="DE" term="%22Insect+embryos%22">Insect embryos</searchLink><br /><searchLink fieldCode="DE" term="%22Povidone%22">Povidone</searchLink><br /><searchLink fieldCode="DE" term="%22Cryoprotective+agents%22">Cryoprotective agents</searchLink><br /><searchLink fieldCode="DE" term="%22Ethylene+glycol%22">Ethylene glycol</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: There is an urgent need to preserve the ever-decreasing number of different species of fireflies all over the world. We sought to develop a vitrification procedure to cryopreserve the firefly embryos. The late stages of Luciola praeusta Kiesenwetter embryos were collected. Several impermeable and permeable protectants with various concentrations in different mediums (TNM-FH insect medium, Grace's medium, Dulbecco's Modification of Eagle's Medium (DMEM) and Dulbecco's Phosphate-Buffered Saline (DPBS)) were used. Embryos culturing in TNM-FH medium yielded the highest survival rate of 75.3 ± 3.6%. One-step, two-step and three-step methods were used in equilibrium procedure respectively. The highest survival rate (73.7% ±3.2%) occurred in embryos treated by three-step method ((1.5 M ethylene glycol (EG) + 2 M EG plus 8% polyvinylpyrrolidone (PVP) + 3 M EG, 8% PVP and 15% trehalose). Additionally, embryos exposed to 0.5 M trehalose presented a significantly higher survival rate (71.8 ± 2.7%) than embryos preserved in 0.5 M sucrose. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.cryobiol.2017.06.005
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 5
        StartPage: 101
    Subjects:
      – SubjectFull: Fireflies
        Type: general
      – SubjectFull: Insect embryos
        Type: general
      – SubjectFull: Povidone
        Type: general
      – SubjectFull: Cryoprotective agents
        Type: general
      – SubjectFull: Ethylene glycol
        Type: general
    Titles:
      – TitleFull: Cryopreservation of Luciola praeusta Kiesenwetter (Coleoptera: Lampyridae) embryos by vitrification.
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            NameFull: Li, Kang
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            NameFull: Chen, Xuanli
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            NameFull: Song, Xiaoling
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            NameFull: Wu, Xi
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            NameFull: Xian, Yi
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            – D: 01
              M: 10
              Text: Oct2017
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              Y: 2017
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              Value: 78
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            – TitleFull: Cryobiology
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